{"id":661,"date":"2015-08-03T21:51:57","date_gmt":"2015-08-03T21:51:57","guid":{"rendered":"https:\/\/courses.candelalearning.com\/bio2labsxmaster2\/?post_type=chapter&#038;p=661"},"modified":"2019-07-24T19:50:35","modified_gmt":"2019-07-24T19:50:35","slug":"community-ecology-lab-instructor-materials-preparation","status":"publish","type":"chapter","link":"https:\/\/courses.lumenlearning.com\/bio2labs\/chapter\/community-ecology-lab-instructor-materials-preparation\/","title":{"raw":"Community Ecology Lab (Instructor Materials Preparation)*","rendered":"Community Ecology Lab (Instructor Materials Preparation)*"},"content":{"raw":"<h2>Lab Materials<\/h2>\r\nThis is the prep for\u00a0<em>one section\u00a0<\/em>of 24 students.\r\n\r\n&nbsp;\r\n<h3>Ecological Succession of Bacteria in Milk<\/h3>\r\nStudents will do this part in table teams (groups of 4).\r\n<table>\r\n<thead>\r\n<tr>\r\n<th>Materials<\/th>\r\n<th>Quantity<\/th>\r\n<th>Notes<\/th>\r\n<\/tr>\r\n<\/thead>\r\n<tbody>\r\n<tr>\r\n<td>\u00a0clean microscope slides<\/td>\r\n<td>\u00a01 box<\/td>\r\n<td><\/td>\r\n<\/tr>\r\n<tr>\r\n<td>\u00a0coverslips<\/td>\r\n<td>\u00a01 box<\/td>\r\n<td><\/td>\r\n<\/tr>\r\n<tr>\r\n<td>Bunsen Burner<\/td>\r\n<td>1 per table<\/td>\r\n<td><\/td>\r\n<\/tr>\r\n<tr>\r\n<td>\u00a0tubing for Bunsen Burners<\/td>\r\n<td>\u00a01 per table<\/td>\r\n<td><\/td>\r\n<\/tr>\r\n<tr>\r\n<td>\u00a0wire loop<\/td>\r\n<td>\u00a01 per table<\/td>\r\n<td><\/td>\r\n<\/tr>\r\n<tr>\r\n<td>50 ml beaker<\/td>\r\n<td>3 per table<\/td>\r\n<td><\/td>\r\n<\/tr>\r\n<tr>\r\n<td>pH probe<\/td>\r\n<td>1 per table<\/td>\r\n<td>can use pH paper as an alternative<\/td>\r\n<\/tr>\r\n<tr>\r\n<td>compound light microscope<\/td>\r\n<td>1-2 per table<\/td>\r\n<td><\/td>\r\n<\/tr>\r\n<tr>\r\n<td>clothespins<\/td>\r\n<td>2 per table<\/td>\r\n<td>to hold slides for gram staining procedure<\/td>\r\n<\/tr>\r\n<tr>\r\n<td>gram staining kit<\/td>\r\n<td>1 per table<\/td>\r\n<td>includes Safranin, Crystal violet, Iodine and ethanol<\/td>\r\n<\/tr>\r\n<tr>\r\n<td>Bunsen striker<\/td>\r\n<td>1 per table<\/td>\r\n<td><\/td>\r\n<\/tr>\r\n<tr>\r\n<td>squirt bottle of DI water<\/td>\r\n<td>1 per table<\/td>\r\n<td><\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\nSide bench for students to share: empty milk containers with nutritional information: whole milk, 2%, 1%, skim, chocolate, whole buttermilk and reduce fat buttermilk\r\n\r\nSide bench for students to share: 1L beaker of 10% bleach solution (to disinfect slides are staining)\r\n\r\n1 week prior to lab purchase the following:\r\n<ul>\r\n\t<li>Whole milk: 1 gallon (red tape)<\/li>\r\n\t<li>2% milk: 1\/2 gallon (white tape)<\/li>\r\n\t<li>1% milk: 1\/2 gallon (green tape)<\/li>\r\n\t<li>Skim milk: 1\/2 gallon (blue tape)<\/li>\r\n\t<li>Chocolate milk; 1\/2 gallon (brown tape)<\/li>\r\n\t<li>Whole buttermilk: 1\/2 gallon (yellow tape)<\/li>\r\n\t<li>Reduced fat buttermilk: 1\/2 gallon (orange tape)<\/li>\r\n<\/ul>\r\nMilk set up:\r\n<ol>\r\n\t<li>Label seven 300 ml clear bottles with colors listed above.<\/li>\r\n\t<li>Pour 100 ml of each milk type intwo their respective bottles<\/li>\r\n\t<li>Place bottles on a cart in the prep area until the first lab<\/li>\r\n\t<li>Repeat steps 1-3 each day until the last day of the lab<\/li>\r\n\t<li>At the end of the two week there should be a total of 70 labeld bottles<\/li>\r\n<\/ol>\r\nMilk in incubator (should be set at 37 C)\r\n<ol>\r\n\t<li>Obtain one 300 ml clear glass bottl<\/li>\r\n\t<li>Pour 100 ml of whole milk into the bottl and place in the incubator (whole milk is the only type to go in the incubator)<\/li>\r\n\t<li>Repeat steps 1-2 each day until the last day of this lab<\/li>\r\n\t<li>At the end of the two week period there should be a total of 10 labeled bottles<\/li>\r\n<\/ol>","rendered":"<h2>Lab Materials<\/h2>\n<p>This is the prep for\u00a0<em>one section\u00a0<\/em>of 24 students.<\/p>\n<p>&nbsp;<\/p>\n<h3>Ecological Succession of Bacteria in Milk<\/h3>\n<p>Students will do this part in table teams (groups of 4).<\/p>\n<table>\n<thead>\n<tr>\n<th>Materials<\/th>\n<th>Quantity<\/th>\n<th>Notes<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>\u00a0clean microscope slides<\/td>\n<td>\u00a01 box<\/td>\n<td><\/td>\n<\/tr>\n<tr>\n<td>\u00a0coverslips<\/td>\n<td>\u00a01 box<\/td>\n<td><\/td>\n<\/tr>\n<tr>\n<td>Bunsen Burner<\/td>\n<td>1 per table<\/td>\n<td><\/td>\n<\/tr>\n<tr>\n<td>\u00a0tubing for Bunsen Burners<\/td>\n<td>\u00a01 per table<\/td>\n<td><\/td>\n<\/tr>\n<tr>\n<td>\u00a0wire loop<\/td>\n<td>\u00a01 per table<\/td>\n<td><\/td>\n<\/tr>\n<tr>\n<td>50 ml beaker<\/td>\n<td>3 per table<\/td>\n<td><\/td>\n<\/tr>\n<tr>\n<td>pH probe<\/td>\n<td>1 per table<\/td>\n<td>can use pH paper as an alternative<\/td>\n<\/tr>\n<tr>\n<td>compound light microscope<\/td>\n<td>1-2 per table<\/td>\n<td><\/td>\n<\/tr>\n<tr>\n<td>clothespins<\/td>\n<td>2 per table<\/td>\n<td>to hold slides for gram staining procedure<\/td>\n<\/tr>\n<tr>\n<td>gram staining kit<\/td>\n<td>1 per table<\/td>\n<td>includes Safranin, Crystal violet, Iodine and ethanol<\/td>\n<\/tr>\n<tr>\n<td>Bunsen striker<\/td>\n<td>1 per table<\/td>\n<td><\/td>\n<\/tr>\n<tr>\n<td>squirt bottle of DI water<\/td>\n<td>1 per table<\/td>\n<td><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>Side bench for students to share: empty milk containers with nutritional information: whole milk, 2%, 1%, skim, chocolate, whole buttermilk and reduce fat buttermilk<\/p>\n<p>Side bench for students to share: 1L beaker of 10% bleach solution (to disinfect slides are staining)<\/p>\n<p>1 week prior to lab purchase the following:<\/p>\n<ul>\n<li>Whole milk: 1 gallon (red tape)<\/li>\n<li>2% milk: 1\/2 gallon (white tape)<\/li>\n<li>1% milk: 1\/2 gallon (green tape)<\/li>\n<li>Skim milk: 1\/2 gallon (blue tape)<\/li>\n<li>Chocolate milk; 1\/2 gallon (brown tape)<\/li>\n<li>Whole buttermilk: 1\/2 gallon (yellow tape)<\/li>\n<li>Reduced fat buttermilk: 1\/2 gallon (orange tape)<\/li>\n<\/ul>\n<p>Milk set up:<\/p>\n<ol>\n<li>Label seven 300 ml clear bottles with colors listed above.<\/li>\n<li>Pour 100 ml of each milk type intwo their respective bottles<\/li>\n<li>Place bottles on a cart in the prep area until the first lab<\/li>\n<li>Repeat steps 1-3 each day until the last day of the lab<\/li>\n<li>At the end of the two week there should be a total of 70 labeld bottles<\/li>\n<\/ol>\n<p>Milk in incubator (should be set at 37 C)<\/p>\n<ol>\n<li>Obtain one 300 ml clear glass bottl<\/li>\n<li>Pour 100 ml of whole milk into the bottl and place in the incubator (whole milk is the only type to go in the incubator)<\/li>\n<li>Repeat steps 1-2 each day until the last day of this lab<\/li>\n<li>At the end of the two week period there should be a total of 10 labeled bottles<\/li>\n<\/ol>\n\n\t\t\t <section class=\"citations-section\" role=\"contentinfo\">\n\t\t\t <h3>Candela Citations<\/h3>\n\t\t\t\t\t <div>\n\t\t\t\t\t\t <div id=\"citation-list-661\">\n\t\t\t\t\t\t\t <div class=\"licensing\"><div class=\"license-attribution-dropdown-subheading\">CC licensed content, Original<\/div><ul class=\"citation-list\"><li>Instructor Materials Preparation. <strong>Authored by<\/strong>: Lynette Hauser. <strong>Provided by<\/strong>: Tidewater Community College. <strong>Located at<\/strong>: <a target=\"_blank\" href=\"http:\/\/www.tcc.edu\/\">http:\/\/www.tcc.edu\/<\/a>. <strong>License<\/strong>: <em><a target=\"_blank\" rel=\"license\" href=\"https:\/\/creativecommons.org\/licenses\/by\/4.0\/\">CC BY: Attribution<\/a><\/em><\/li><li>Community Ecology Lab. <strong>Authored by<\/strong>: Dr. William Edwards. <strong>Provided by<\/strong>: Niagara University. <strong>Located at<\/strong>: <a target=\"_blank\" href=\"https:\/\/www.niagara.edu\/\">https:\/\/www.niagara.edu\/<\/a>. <strong>License<\/strong>: <em><a target=\"_blank\" rel=\"license\" href=\"https:\/\/creativecommons.org\/licenses\/by\/4.0\/\">CC BY: Attribution<\/a><\/em><\/li><\/ul><\/div>\n\t\t\t\t\t\t <\/div>\n\t\t\t\t\t <\/div>\n\t\t\t <\/section>","protected":false},"author":78,"menu_order":2,"template":"","meta":{"_candela_citation":"[{\"type\":\"original\",\"description\":\"Instructor Materials Preparation\",\"author\":\"Lynette Hauser\",\"organization\":\"Tidewater Community College\",\"url\":\"http:\/\/www.tcc.edu\/\",\"project\":\"\",\"license\":\"cc-by\",\"license_terms\":\"\"},{\"type\":\"original\",\"description\":\"Community Ecology Lab\",\"author\":\"Dr. William Edwards\",\"organization\":\"Niagara University\",\"url\":\"https:\/\/www.niagara.edu\/\",\"project\":\"\",\"license\":\"cc-by\",\"license_terms\":\"\"}]","CANDELA_OUTCOMES_GUID":"","pb_show_title":"on","pb_short_title":"","pb_subtitle":"","pb_authors":[],"pb_section_license":""},"chapter-type":[],"contributor":[],"license":[],"class_list":["post-661","chapter","type-chapter","status-publish","hentry"],"part":213,"_links":{"self":[{"href":"https:\/\/courses.lumenlearning.com\/bio2labs\/wp-json\/pressbooks\/v2\/chapters\/661","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/courses.lumenlearning.com\/bio2labs\/wp-json\/pressbooks\/v2\/chapters"}],"about":[{"href":"https:\/\/courses.lumenlearning.com\/bio2labs\/wp-json\/wp\/v2\/types\/chapter"}],"author":[{"embeddable":true,"href":"https:\/\/courses.lumenlearning.com\/bio2labs\/wp-json\/wp\/v2\/users\/78"}],"version-history":[{"count":6,"href":"https:\/\/courses.lumenlearning.com\/bio2labs\/wp-json\/pressbooks\/v2\/chapters\/661\/revisions"}],"predecessor-version":[{"id":782,"href":"https:\/\/courses.lumenlearning.com\/bio2labs\/wp-json\/pressbooks\/v2\/chapters\/661\/revisions\/782"}],"part":[{"href":"https:\/\/courses.lumenlearning.com\/bio2labs\/wp-json\/pressbooks\/v2\/parts\/213"}],"metadata":[{"href":"https:\/\/courses.lumenlearning.com\/bio2labs\/wp-json\/pressbooks\/v2\/chapters\/661\/metadata\/"}],"wp:attachment":[{"href":"https:\/\/courses.lumenlearning.com\/bio2labs\/wp-json\/wp\/v2\/media?parent=661"}],"wp:term":[{"taxonomy":"chapter-type","embeddable":true,"href":"https:\/\/courses.lumenlearning.com\/bio2labs\/wp-json\/pressbooks\/v2\/chapter-type?post=661"},{"taxonomy":"contributor","embeddable":true,"href":"https:\/\/courses.lumenlearning.com\/bio2labs\/wp-json\/wp\/v2\/contributor?post=661"},{"taxonomy":"license","embeddable":true,"href":"https:\/\/courses.lumenlearning.com\/bio2labs\/wp-json\/wp\/v2\/license?post=661"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}