{"id":204,"date":"2015-07-13T20:41:25","date_gmt":"2015-07-13T20:41:25","guid":{"rendered":"https:\/\/courses.candelalearning.com\/biolabsxmaster\/?post_type=chapter&p=204"},"modified":"2019-07-24T19:51:28","modified_gmt":"2019-07-24T19:51:28","slug":"dna-structure-and-function-instructor-materials-preparation","status":"publish","type":"chapter","link":"https:\/\/courses.lumenlearning.com\/biolabs1\/chapter\/dna-structure-and-function-instructor-materials-preparation\/","title":{"raw":"DNA Structure and Function (Instructor Materials Preparation)","rendered":"DNA Structure and Function (Instructor Materials Preparation)"},"content":{"raw":"

Lab Materials<\/h2>\r\nThis is the prep for one section <\/em>of 24 students.\r\n
\r\n

Part 4: Wheat Germ Extraction<\/h3>\r\nStudents will do this part in pairs.\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n
Materials<\/th>\r\nQuantity<\/th>\r\nNotes<\/th>\r\n<\/tr>\r\n<\/thead>\r\n
Beaker (250 mL)<\/td>\r\n1 per table<\/td>\r\n<\/td>\r\n<\/tr>\r\n
Graduated cylinder (10 mL)<\/td>\r\n1 per table<\/td>\r\n<\/td>\r\n<\/tr>\r\n
Graduated cylinder (100 mL)<\/td>\r\n1 per table<\/td>\r\n<\/td>\r\n<\/tr>\r\n
Serological pipettes (5 mL)<\/td>\r\n1 per table<\/td>\r\n2 additional for sodium bicarbonate solution<\/td>\r\n<\/tr>\r\n
Pipettors (green)<\/td>\r\n1 per table<\/td>\r\n2 additional for sodium bicarbonate solution<\/td>\r\n<\/tr>\r\n
Thermometers<\/td>\r\n1 per table<\/td>\r\n<\/td>\r\n<\/tr>\r\n
Dawn dish detergent<\/td>\r\n1 per table<\/td>\r\nSmall personal container<\/td>\r\n<\/tr>\r\n
Adolf's Meat Tenderizer (original unseasoned)<\/td>\r\n2 containers<\/td>\r\nOn side counter<\/td>\r\n<\/tr>\r\n
Scoops for meat tenderizer<\/td>\r\n2<\/td>\r\nOn side counter<\/td>\r\n<\/tr>\r\n
Raw untoasted wheat germ<\/td>\r\n2 containers<\/td>\r\nOn side counter<\/td>\r\n<\/tr>\r\n
Digital balance<\/td>\r\n2<\/td>\r\nOn side counter<\/td>\r\n<\/tr>\r\n
Weigh papers<\/td>\r\n<\/td>\r\nOn side counter<\/td>\r\n<\/tr>\r\n
1 M sodium bicarbonate<\/td>\r\n~2 bottles<\/td>\r\nEach pair will need 5 mL<\/td>\r\n<\/tr>\r\n
Waste buckets for wheat germ<\/td>\r\n2<\/td>\r\n<\/td>\r\n<\/tr>\r\n
Signs\u00a0across sinks: \"No used\u00a0wheat germ disposal or you will be\u00a0destroyed!\"<\/td>\r\n<\/td>\r\n<\/td>\r\n<\/tr>\r\n
Glass DNA hooks<\/td>\r\n6<\/td>\r\nAt instructor's station<\/td>\r\n<\/tr>\r\n
Ice bath<\/td>\r\n<\/td>\r\nOn side counter; large enough for 10 beakers<\/td>\r\n<\/tr>\r\n
Hot bath<\/td>\r\n<\/td>\r\nOn side counter with thermometer; 55\u00ba C<\/td>\r\n<\/tr>\r\n
Alcohol (95% in freezer)<\/td>\r\n20 mL per table<\/td>\r\nOn side counter in ice bath<\/td>\r\n<\/tr>\r\n
Glass stirring rods<\/td>\r\n1 per table<\/td>\r\n<\/td>\r\n<\/tr>\r\n
Wax pencils<\/td>\r\n1 per table<\/td>\r\n<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n<\/div>","rendered":"

Lab Materials<\/h2>\n

This is the prep for one section <\/em>of 24 students.<\/p>\n

\n

Part 4: Wheat Germ Extraction<\/h3>\n

Students will do this part in pairs.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Materials<\/th>\nQuantity<\/th>\nNotes<\/th>\n<\/tr>\n<\/thead>\n
Beaker (250 mL)<\/td>\n1 per table<\/td>\n<\/td>\n<\/tr>\n
Graduated cylinder (10 mL)<\/td>\n1 per table<\/td>\n<\/td>\n<\/tr>\n
Graduated cylinder (100 mL)<\/td>\n1 per table<\/td>\n<\/td>\n<\/tr>\n
Serological pipettes (5 mL)<\/td>\n1 per table<\/td>\n2 additional for sodium bicarbonate solution<\/td>\n<\/tr>\n
Pipettors (green)<\/td>\n1 per table<\/td>\n2 additional for sodium bicarbonate solution<\/td>\n<\/tr>\n
Thermometers<\/td>\n1 per table<\/td>\n<\/td>\n<\/tr>\n
Dawn dish detergent<\/td>\n1 per table<\/td>\nSmall personal container<\/td>\n<\/tr>\n
Adolf’s Meat Tenderizer (original unseasoned)<\/td>\n2 containers<\/td>\nOn side counter<\/td>\n<\/tr>\n
Scoops for meat tenderizer<\/td>\n2<\/td>\nOn side counter<\/td>\n<\/tr>\n
Raw untoasted wheat germ<\/td>\n2 containers<\/td>\nOn side counter<\/td>\n<\/tr>\n
Digital balance<\/td>\n2<\/td>\nOn side counter<\/td>\n<\/tr>\n
Weigh papers<\/td>\n<\/td>\nOn side counter<\/td>\n<\/tr>\n
1 M sodium bicarbonate<\/td>\n~2 bottles<\/td>\nEach pair will need 5 mL<\/td>\n<\/tr>\n
Waste buckets for wheat germ<\/td>\n2<\/td>\n<\/td>\n<\/tr>\n
Signs\u00a0across sinks: “No used\u00a0wheat germ disposal or you will be\u00a0destroyed!”<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Glass DNA hooks<\/td>\n6<\/td>\nAt instructor’s station<\/td>\n<\/tr>\n
Ice bath<\/td>\n<\/td>\nOn side counter; large enough for 10 beakers<\/td>\n<\/tr>\n
Hot bath<\/td>\n<\/td>\nOn side counter with thermometer; 55\u00ba C<\/td>\n<\/tr>\n
Alcohol (95% in freezer)<\/td>\n20 mL per table<\/td>\nOn side counter in ice bath<\/td>\n<\/tr>\n
Glass stirring rods<\/td>\n1 per table<\/td>\n<\/td>\n<\/tr>\n
Wax pencils<\/td>\n1 per table<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n\n\t\t\t
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Candela Citations<\/h3>\n\t\t\t\t\t
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CC licensed content, Original<\/div>