The Structure and Sequence of DNA
DNA is a double helix of two anti-parallel, complementary strands having a phosphate-sugar backbone with nitrogenous bases stacked inside.
Describe the structure of DNA and summarize the importance of DNA sequencing
- The two DNA strands are anti-parallel in nature; that is, the 3′ end of one strand faces the 5′ end of the other strand.
- The nucleotides that comprise DNA contain a nitrogenous base, a deoxyribose sugar, and a phosphate group which covalently link with other nucleotides to form phosphodiester bonds.
- Nucleotide bases can be classified as purines (containing a double-ring structure) or pyrimidines (containing a single-ring structure).
- Adenine (purine) and thymine (pyrimidine) are complementary base pairs as are guanine (purine) and cytosine (pyrimidine).
- DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule.
- deoxyribose: a derivative of the pentose sugar ribose in which the 2′ hydroxyl (-OH) is reduced to a hydrogen (H); a constituent of the nucleotides that comprise deoxyribonucleic acid, or DNA
- hydrogen bond: A weak bond in which a hydrogen atom already covalently bonded to a oxygen or nitrogen atom in one molecule is attracted to an electronegative atom (usually nitrogen or oxygen) in the same or different molecule.
- nucleotide: the monomer comprising DNA or RNA molecules; consists of a nitrogenous heterocyclic base that can be a purine or pyrimidine, a five-carbon pentose sugar, and a phosphate group
The monomeric building blocks of DNA are deoxyribomononucleotides (usually referred to as just nucleotides), and DNA is formed from linear chains, or polymers, of these nucleotides. The components of the nucleotide used in DNA synthesis are a nitrogenous base, a deoxyribose, and a phosphate group. The nucleotide is named depending on which nitrogenous base is present. The nitrogenous base can be a purine such as adenine (A) and guanine (G), characterized by double-ring structures, or a pyrimidine such as cytosine (C) and thymine (T), characterized by single-ring structures. In polynucleotides (the linear polymers of nucleotides) the nucleotides are connected to each other by covalent bonds known as phosphodiester bonds or phosphodiester linkages.
James Watson and Francis Crick, with some help from Rosalind Franklin and Maurice Wilkins, are credited with figuring out the structure of DNA. Watson and Crick proposed that DNA is made up of two polynucleotide strands that are twisted around each other to form a right-handed helix.
The two polynucleotide strands are anti-parallel in nature. That is, they run in opposite directions.
The sugars and phosphates of the nucleotides form the backbone of the structure, whereas the pairs of nitrogenous bases are pointed towards the interior of the molecule.
The twisting of the two strands around each other results in the formation of uniformly-spaced major and minor grooves bordered by the sugar-phosphate backbones of the two strands.
The diameter of the DNA double helix is 2 nm and is uniform throughout. Only the pairing between a purine and pyrimidine can explain the uniform diameter. That is to say, at each point along the DNA molecule, the two sugar phosphate backbones are always separated by three rings, two from a purine and one from a pyrimidine.
The two strands are held together by base pairing between nitrogenous bases of one strand and nitrogenous bases from the other strand. Base pairing takes place between a purine and pyrimidine stabilized by hydrogen bonds: A pairs with T via two hydrogen bonds and G pairs with C via three hydrogen bonds.
The interior basepairs rotate with respect to one another, but are also stacked on top of each other when the molecule is viewed looking up or down its long axis.
Each base pair is separated from the previous base pair by a height of 0.34 nm and each 360o turn of the helix travels 3.4 nm along the long axis of the molecule. Therefore, ten base pairs are present per turn of the helix.
DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. Rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery. Knowledge of DNA sequences has become indispensable for basic biological research, and in numerous applied fields such as diagnostics, biotechnology, forensic biology, and biological systematics. The rapid speed of sequencing attained with modern technology has been instrumental in obtaining complete DNA sequences, or genomes, of numerous types and species of life, including the human genome and those of other animal, plant, and microbial species.