Peroxisomes neutralize harmful toxins and carry out lipid metabolism and oxidation reactions that break down fatty acids and amino acids.
Name the various functions that peroxisomes perform inside the cell
- Lipid metabolism and chemical detoxification are important functions of peroxisomes.
- Peroxisomes are responsible for oxidation reactions that break down fatty acids and amino acids.
- Peroxisomes oversee reactions that neutralize free radicals, which cause cellular damage and cell death.
- Peroxisomes chemically neutralize poisons through a process that produces large amounts of toxic H2O2, which is then converted into water and oxygen.
- The liver is the organ primarily responsible for detoxifying the blood before it travels throughout the body; as a result, liver cells contain large amounts of peroxisomes.
- enzyme: a globular protein that catalyses a biological chemical reaction
- free radical: Any molecule, ion or atom that has one or more unpaired electrons; they are generally highly reactive and often only occur as transient species.
A type of organelle found in both animal cells and plant cells, a peroxisome is a membrane-bound cellular organelle that contains mostly enzymes. Peroxisomes perform important functions, including lipid metabolism and chemical detoxification. They also carry out oxidation reactions that break down fatty acids and amino acids.
In contrast to the digestive enzymes found in lysosomes, the enzymes within peroxisomes serve to transfer hydrogen atoms from various molecules to oxygen, producing hydrogen peroxide (H2O2). In this way, peroxisomes neutralize poisons, such as alcohol, that enter the body. In order to appreciate the importance of peroxisomes, it is necessary to understand the concept of reactive oxygen species.
Reactive oxygen species (ROS), such as peroxides and free radicals, are the highly-reactive products of many normal cellular processes, including the mitochondrial reactions that produce ATP and oxygen metabolism. Examples of ROS include the hydroxyl radical OH, H2O2, and superoxide (O−2). Some ROS are important for certain cellular functions, such as cell signaling processes and immune responses against foreign substances. Many ROS, however, are harmful to the body. Free radicals are reactive because they contain free unpaired electrons; they can easily oxidize other molecules throughout the cell, causing cellular damage and even cell death. Free radicals are thought to play a role in many destructive processes in the body, from cancer to coronary artery disease.
Peroxisomes oversee reactions that neutralize free radicals. They produce large amounts of the toxic H2O2 in the process, but contain enzymes that convert H2O2 into water and oxygen. These by-products are then safely released into the cytoplasm. Like miniature sewage treatment plants, peroxisomes neutralize harmful toxins so that they do not cause damage in the cells. The liver is the organ primarily responsible for detoxifying the blood before it travels throughout the body; liver cells contain an exceptionally high number of peroxisomes.
Lysosomes are organelles that digest macromolecules, repair cell membranes, and respond to foreign substances entering the cell.
Describe how lysosomes function as the cell’s waste disposal system
- Lysosomes breakdown/digest macromolecules (carbohydrates, lipids, proteins, and nucleic acids), repair cell membranes, and respond against foreign substances such as bacteria, viruses and other antigens.
- Lysosomes contain enzymes that break down the macromolecules and foreign invaders.
- Lysosomes are composed of lipids and proteins, with a single membrane covering the internal enzymes to prevent the lysosome from digesting the cell itself.
- Lysosomes are found in all animal cells, but are rarely found within plant cells due to the tough cell wall surrounding a plant cell that keeps out foreign substances.
- enzyme: a globular protein that catalyses a biological chemical reaction
- lysosome: An organelle found in all types of animal cells which contains a large range of digestive enzymes capable of splitting most biological macromolecules.
A lysosome has three main functions: the breakdown/digestion of macromolecules (carbohydrates, lipids, proteins, and nucleic acids), cell membrane repairs, and responses against foreign substances such as bacteria, viruses and other antigens. When food is eaten or absorbed by the cell, the lysosome releases its enzymes to break down complex molecules including sugars and proteins into usable energy needed by the cell to survive. If no food is provided, the lysosome’s enzymes digest other organelles within the cell in order to obtain the necessary nutrients.
In addition to their role as the digestive component and organelle-recycling facility of animal cells, lysosomes are considered to be parts of the endomembrane system. Lysosomes also use their hydrolytic enzymes to destroy pathogens (disease-causing organisms) that might enter the cell. A good example of this occurs in a group of white blood cells called macrophages, which are part of your body’s immune system. In a process known as phagocytosis or endocytosis, a section of the plasma membrane of the macrophage invaginates (folds in) and engulfs a pathogen. The invaginated section, with the pathogen inside, then pinches itself off from the plasma membrane and becomes a vesicle. The vesicle fuses with a lysosome. The lysosome’s hydrolytic enzymes then destroy the pathogen.
A lysosome is composed of lipids, which make up the membrane, and proteins, which make up the enzymes within the membrane. Usually, lysosomes are between 0.1 to 1.2μm, but the size varies based on the cell type. The general structure of a lysosome consists of a collection of enzymes surrounded by a single-layer membrane. The membrane is a crucial aspect of its structure because without it the enzymes within the lysosome that are used to breakdown foreign substances would leak out and digest the entire cell, causing it to die.
Lysosomes are found in nearly every animal-like eukaryotic cell. They are so common in animal cells because, when animal cells take in or absorb food, they need the enzymes found in lysosomes in order to digest and use the food for energy. On the other hand, lysosomes are not commonly-found in plant cells. Lysosomes are not needed in plant cells because they have cell walls that are tough enough to keep the large/foreign substances that lysosomes would usually digest out of the cell.
Intermediate Filaments and Microtubules
Microtubules are part of the cell’s cytoskeleton, helping the cell resist compression, move vesicles, and separate chromosomes at mitosis.
Describe the roles of microtubules as part of the cell’s cytoskeleton
- Microtubules help the cell resist compression, provide a track along which vesicles can move throughout the cell, and are the components of cilia and flagella.
- Cilia and flagella are hair-like structures that assist with locomotion in some cells, as well as line various structures to trap particles.
- The structures of cilia and flagella are a “9+2 array,” meaning that a ring of nine microtubules is surrounded by two more microtubules.
- Microtubules attach to replicated chromosomes during cell division and pull them apart to opposite ends of the pole, allowing the cell to divide with a complete set of chromosomes in each daughter cell.
- microtubule: Small tubes made of protein and found in cells; part of the cytoskeleton
- flagellum: a flagellum is a lash-like appendage that protrudes from the cell body of certain prokaryotic and eukaryotic cells
- cytoskeleton: A cellular structure like a skeleton, contained within the cytoplasm.
As their name implies, microtubules are small hollow tubes. Microtubules, along with microfilaments and intermediate filaments, come under the class of organelles known as the cytoskeleton. The cytoskeleton is the framework of the cell which forms the structural supporting component. Microtubules are the largest element of the cytoskeleton. The walls of the microtubule are made of polymerized dimers of α-tubulin and β-tubulin, two globular proteins. With a diameter of about 25 nm, microtubules are the widest components of the cytoskeleton. They help the cell resist compression, provide a track along which vesicles move through the cell, and pull replicated chromosomes to opposite ends of a dividing cell. Like microfilaments, microtubules can dissolve and reform quickly.
Microtubules are also the structural elements of flagella, cilia, and centrioles (the latter are the two perpendicular bodies of the centrosome ). In animal cells, the centrosome is the microtubule-organizing center. In eukaryotic cells, flagella and cilia are quite different structurally from their counterparts in prokaryotes.
Intermediate filaments (IFs) are cytoskeletal components found in animal cells. They are composed of a family of related proteins sharing common structural and sequence features. Intermediate filaments have an average diameter of 10 nanometers, which is between that of 7 nm actin (microfilaments), and that of 25 nm microtubules, although they were initially designated ‘intermediate’ because their average diameter is between those of narrower microfilaments (actin) and wider myosin filaments found in muscle cells. Intermediate filaments contribute to cellular structural elements and are often crucial in holding together tissues like skin.
Flagella and Cilia
Flagella (singular = flagellum ) are long, hair-like structures that extend from the plasma membrane and are used to move an entire cell (for example, sperm, Euglena). When present, the cell has just one flagellum or a few flagella. When cilia (singular = cilium) are present, however, many of them extend along the entire surface of the plasma membrane. They are short, hair-like structures that are used to move entire cells (such as paramecia) or substances along the outer surface of the cell (for example, the cilia of cells lining the Fallopian tubes that move the ovum toward the uterus, or cilia lining the cells of the respiratory tract that trap particulate matter and move it toward your nostrils).
Despite their differences in length and number, flagella and cilia share a common structural arrangement of microtubules called a “9 + 2 array.” This is an appropriate name because a single flagellum or cilium is made of a ring of nine microtubule doublets surrounding a single microtubule doublet in the center.
Extracellular Matrix of Animal Cells
The extracellular matrix of animal cells holds cells together to form a tissue and allow tissues to communicate with each other.
Explain the role of the extracellular matrix in animal cells
- The extracellular matrix of animal cells is made up of proteins and carbohydrates.
- Cell communication within tissue and tissue formation are main functions of the extracellular matrix of animal cells.
- Tissue communication is kick-started when a molecule within the matrix binds a receptor; the end results are conformational changes that induce chemical signals that ultimately change activities within the cell.
- collagen: Any of more than 28 types of glycoprotein that forms elongated fibers, usually found in the extracellular matrix of connective tissue.
- proteoglycan: Any of many glycoproteins that have heteropolysaccharide side chains
- extracellular matrix: All the connective tissues and fibres that are not part of a cell, but rather provide support.
Extracellular Matrix of Animal Cells
Most animal cells release materials into the extracellular space. The primary components of these materials are proteins. Collagen is the most abundant of the proteins. Its fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. Collectively, these materials are called the extracellular matrix. Not only does the extracellular matrix hold the cells together to form a tissue, but it also allows the cells within the tissue to communicate with each other.
How does this cell communication occur? Cells have protein receptors on the extracellular surfaces of their plasma membranes. When a molecule within the matrix binds to the receptor, it changes the molecular structure of the receptor. The receptor, in turn, changes the conformation of the microfilaments positioned just inside the plasma membrane. These conformational changes induce chemical signals inside the cell that reach the nucleus and turn “on” or “off” the transcription of specific sections of DNA. This affects the production of associated proteins, thus changing the activities within the cell.
An example of the role of the extracellular matrix in cell communication can be seen in blood clotting. When the cells lining a blood vessel are damaged, they display a protein receptor called tissue factor. When a tissue factor binds with another factor in the extracellular matrix, it causes platelets to adhere to the wall of the damaged blood vessel and stimulates the adjacent smooth muscle cells in the blood vessel to contract (thus constricting the blood vessel). Subsequently, a series of steps are initiated which then prompt the platelets to produce clotting factors.