An electron microscope is a microscope that uses an electron beam to create an image of the target.
Explain why electron microscopes provide higher resolution than optical microscopes
- Electron microscopes are very useful as they are able to magnify objects to a much higher resolution than optical ones.
- Higher resolution can be achieved with electron microscopes because the de Broglie wavelengths for electrons are so much smaller than that of visible light.
- In electron microscopes, electromagnets can be used as magnetic lenses to manipulate electron beams.
- CCD: A charge-coupled device (CCD) is a device for the movement of electrical charge, usually from within the device to an area where the charge can be manipulated, for example conversion into a digital value. The CCD is a major technology required for digital imaging.
- de Broglie wavelength: The wavelength of a matter wave is inversely proportional to the momentum of a particle and is called a de Broglie wavelength.
We have seen that under certain circumstances particles behave like waves. This idea is used in the electron microscope which is a type that uses electrons to create an image of the target. It has much higher magnification or resolving power than a normal light microscope. It can achieve better than 50 pm resolution and magnifications of up to about 10,000,000 times, whereas ordinary, nonconfocal light microscopes are limited by diffraction to about 200 nm resolution and useful magnifications below 2000 times.
Let’s first review how a regular optical microscope works. A beam of light is shone through a thin target and the image is then magnified and focused using objective and ocular lenses. The amount of light which passes through the target depends on its densities, since the less dense regions allow more light to pass through than the denser regions. This means that the beam of light which is partially transmitted through the target carries information about the inner structure of the target.
The original form of electron microscopy, transmission electron microscopy, works in a similar manner using electrons. In the electron microscope, electrons which are emitted by a cathode are formed into a beam using magnetic lenses (usually electromagnets). This electron beam is then passed through a very thin target. Again, the regions in the target with higher densities stop the electrons more easily. So, the number of electrons which pass through the different regions of the target depends on their densities. This means that the partially transmitted beam of electrons carries information about the densities of the inner structure of the target.
The spatial variation in this information (the “image”) is then magnified by a series of magnetic lenses and it is recorded by hitting a fluorescent screen, photographic plate, or light-sensitive sensor such as a CCD (charge-coupled device) camera. The image detected by the CCD may be displayed in real time on a monitor or computer.
Electron microscopes are very useful as they are able to magnify objects to a much higher resolution. This is because their de Broglie wavelengths are so much smaller than that of visible light. You hopefully remember that light is diffracted by objects which are separated by a distance of about the same size as the wavelength of the light. This diffraction then prevents you from being able to focus the transmitted light into an image.
Therefore, the sizes at which diffraction occurs for a beam of electrons is much smaller than those for visible light. This is why you can magnify targets to a much higher order of magnification using electrons rather than visible light.
A laser consists of a gain medium, a mechanism to supply energy to it, and something to provide optical feedback.
Describe basic parts of laser
- The gain medium is where the optical amplification process occurs. Gas and semiconductors are commonly used gain media.
- The most common type of laser uses feedback from an optical cavity–a pair of highly reflective mirrors on either end of the gain medium. A single photon can bounce back and forth between the mirrors many times, passing through the gain medium and being amplified each time.
- Lasers are ubiquitous, finding utility in thousands of highly varied applications in every section of modern society.
- stimulated emission: The process by which an atomic electron (or an excited molecular state) interacting with an electromagnetic wave of a certain frequency may drop to a lower energy level, transferring its energy to that field.
When lasers were invented in 1960, they were called “a solution looking for a problem. ” Nowadays, lasers are ubiquitous, finding utility in thousands of highly varied applications in every section of modern society, including consumer electronics, information technology, science, medicine, industry, law enforcement, entertainment, and the military.
Having examined stimulated emission and optical amplification process in the “Lasers, Applications of Quantum Mechanics” section, this atom looks at how lasers are built.
A laser consists of a gain medium, a mechanism to supply energy to it, and something to provide optical feedback (usually an optical cavity). When a gain medium is placed in an optical cavity, a laser can then produce a coherent beam of photons.
The gain medium is where the optical amplification process occurs. It is excited by an external source of energy into an excited state (called “population inversion”), ready to be fired when a photon with the right frequency enters the medium. In most lasers, this medium consists of a population of atoms which have been excited by an outside light source or an electrical field which supplies energy for atoms to absorb in order to be transformed into excited states. There are many types of lasers depending on the gain media and mode of operation. Gas and semiconductors are commonly used gain media.
The most common type of laser uses feedback from an optical cavity–a pair of highly reflective mirrors on either end of the gain medium. A single photon can bounce back and forth between the mirrors many times, passing through the gain medium and being amplified each time. Typically one of the two mirrors, the output coupler, is partially transparent. Some of the light escapes through this mirror, producing a laser beam that is visible to the naked eye.