Major Enzymes

Learning Outcomes

Identify the major enzymes that play a role in DNA replication

The process of DNA replication is catalyzed by a type of enzyme called DNA polymerase (poly meaning many, mer meaning pieces, and –ase meaning enzyme; so an enzyme that attaches many pieces of DNA). Observe Figure 1: the double helix of the original DNA molecule separates (blue) and new strands are made to match the separated strands. The result will be two DNA molecules, each containing an old and a new strand. Therefore, DNA replication is called semiconservative. The term semiconservative refers to the fact that half of the original molecule (one of the two strands in the double helix) is “conserved” in the new molecule. The original strand is referred to as the template strand because it provides the information, or template, for the newly synthesized strand.

Stylized DNA replication fork with nucleotides matched, 5'->3' synthesis shown, no enzymes in diagram.

Figure 1. By Madprime(wikipedia) (DNA replication split horizontal) CC BY-SA 2.0

Diagram of a primer moving along the template strand of DNA.

Figure 2. Primer and Template

DNA polymerase needs an “anchor” to start adding nucleotides: a short sequence of DNA or RNA that is complementary to the template strand will work to provide a free 3′ end. This sequence is called a primer (Figure 2).

How does DNA polymerase know in what order to add nucleotides? Specific base pairing in DNA is the key to copying the DNA: if you know the sequence of one strand, you can use base pairing rules to build the other strand. Bases form pairs (base pairs) in a very specific way.

Diagram showing the hydrogen bonds between nucleotides. Adenine is bound to thymine, and cytosine is bound to guanine.

Figure 3. DNA chemical structure. Modification of DNA chemical structure by Madeleine Price Ball; CC-BY-SA-2.0

Practice Questions

True/False: DNA replication requires an enzyme.

What are the building blocks on DNA?

  1. Deoxyribonucleotides
  2. Fatty acids
  3. Ribonucleotides
  4. Amino acids

True/False: DNA replication requires energy.

We have the building blocks, a source of energy, and a catalyst. What’s missing? We need instruction about the order of nucleotides in the new polymer. Which molecule provides these instructions?

  1. Protein
  2. DNA
  3. Carbohydrate
  4. Lipid

There is one more thing required by the DNA polymerase. It cannot just start making a DNA copy of the template strand; it needs a short piece of DNA or RNA with a free hydroxyl group in the right place to attach the nucleotides to. (Remember that synthesis always occurs in one direction—new building blocks are added to the 3′ end.) This component starts the process by giving DNA polymerase something to bind to. What might you call this short piece of nucleic acid?

  1. A solvent
  2. A primer
  3. A converter
  4. A sealant

Now that you understand the basics of DNA replication, we can add a bit of complexity. The two strands of DNA have to be temporarily separated from each other; this job is done by a special enzyme, helicase, that helps unwind and separate the DNA helices (Figure 4). Another issue is that the DNA polymerase only works in one direction along the strand (5′ to 3′), but the double-stranded DNA has two strands oriented in opposite directions. This problem is solved by synthesizing the two strands slightly differently: one new strand grows continuously, the other in bits and pieces. Short fragments of RNA are used as primers for the DNA polymerase.

Diagram of both the leading and lagging strands the helicase splits the two strands and a DNA polymerase travels over both strands to create complementary strands.

Figure 4. By Mariana Ruiz (DNA replication) Public Domain

Practice Questions

Which of these separates the two complementary strands of DNA?

  1. DNA polymerase
  2. helicase
  3. RNA primer
  4. single-strand binding protein

Which of these attaches complementary bases to the template strand?

  1. DNA polymerase
  2. helicase
  3. RNA primer
  4. single-strand binding protein

Which of these is later replaced with DNA bases?

  1. DNA polymerase
  2. helicase
  3. RNA primer
  4. single-strand binding protein

In Summary: Major Enzymes

Replication in eukaryotes starts at multiple origins of replication. A primer is required to initiate synthesis, which is then extended by DNA polymerase as it adds nucleotides one by one to the growing chain. The leading strand is synthesized continuously, whereas the lagging strand is synthesized in short stretches called Okazaki fragments. The RNA primers are replaced with DNA nucleotides; the DNA remains one continuous strand by linking the DNA fragments with DNA ligase. Below is a summary table of the major enzymes addressed in this reading, listed in rough order of activity during replication.

Important Enzymes in DNA Replication
Enzyme Function
Topoisomerase Relaxes the super-coiled DNA
DNA helicase Unwinds the double helix at the replication fork
Primase Provides the starting point for DNA polymerase to begin synthesis of the new strand
DNA polymerase Synthesizes the new DNA strand; also proofreads and corrects some errors
DNA ligase Re-joins the two DNA strands into a double helix and joins Okazaki fragments of the lagging strand