{"id":4955,"date":"2019-05-20T12:49:01","date_gmt":"2019-05-20T12:49:01","guid":{"rendered":"https:\/\/courses.lumenlearning.com\/suny-dutchess-ap1\/?post_type=chapter&#038;p=4955"},"modified":"2019-08-30T13:14:24","modified_gmt":"2019-08-30T13:14:24","slug":"protein-synthesis","status":"publish","type":"chapter","link":"https:\/\/courses.lumenlearning.com\/suny-dutchess-ap1\/chapter\/protein-synthesis\/","title":{"raw":"Genes to proteins: Central Dogma","rendered":"Genes to proteins: Central Dogma"},"content":{"raw":"<div class=\"clearfix\">\r\n<div class=\"perseus-renderer perseus-renderer-responsive\">\r\n<div class=\"paragraph\">\r\n<h2>Genes specify functional products (such as proteins)<\/h2>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">A DNA molecule is divided up into functional units called\u00a0<strong>genes<\/strong>. Each gene provides instructions for a functional product, that is, a molecule needed to perform a job in the cell. In many cases, the functional product of a gene is a protein. For example, Mendel's flower color gene provides instructions for a protein that helps make colored molecules (pigments) in flower petals.<\/div>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">\r\n<div class=\"perseus-widget-container widget-nohighlight widget-block\">\r\n<div class=\"perseus-image-widget\">\r\n<div class=\"fixed-to-responsive zoomable svg-image\">\r\n<div><\/div>\r\n<img class=\"alignnone\" title=\"Image based on experimental data reported by Hellens et al.^1 1 start superscript, 1, end superscript and on similar figure in Reece et al.^2 2 start superscript, 2, end superscript.\" src=\"https:\/\/cdn.kastatic.org\/ka-perseus-images\/53b7ece60303244264411d03bfbe55d36312b64e.png\" alt=\"Diagram of how a gene can dictate a phenotype (observable feature) of an organism. The flower color gene that Mendel studied consists of a stretch of DNA found on a chromosome. The DNA has a particular sequence; part of it, shown in this diagram, is 5'-GTAAATCG-3' (upper strand), paired with the complementary sequence 3'-CATTTAGC-5' (lower strand). The DNA of the gene specifies production of a protein that helps make pigments. When the protein is present and functional, pigments are produced, and the flowers of a plant have a purple color.\" width=\"2100\" height=\"404\" \/>\r\n\r\n<\/div>\r\n<div class=\"perseus-renderer perseus-renderer-responsive\">\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">The flower color gene that Mendel studied consists of a stretch of DNA found on a chromosome. The DNA has a particular sequence; part of it, shown in this diagram, is GTAAATCG (upper strand), paired with the complementary sequence CATTTAGC (lower strand). The DNA of the gene specifies production of a protein that helps make pigments. When the protein is present and functional, pigments are produced, and the flowers of a plant have a purple color.<\/div>\r\n<div><\/div>\r\n<\/div>\r\n<\/div>\r\n<div class=\"perseus-image-caption\">\r\n<div class=\"perseus-renderer perseus-renderer-responsive\">\r\n<div class=\"paragraph\"><\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">The functional products of most known genes are proteins, or, more accurately, polypeptides.\u00a0<strong>Polypeptide<\/strong>\u00a0is just another word for a chain of amino acids. Although many proteins consist of a single polypeptide, some are made up of multiple polypeptides. Genes that specify polypeptides are called\u00a0<strong>protein-coding<\/strong>\u00a0genes.\u00a0<span style=\"font-size: 1em\">Not all genes specify polypeptides. Instead, some provide instructions to build functional RNA molecules, such as the\u00a0<\/span><a class=\"_8gcxk83\" style=\"font-size: 1em\" href=\"https:\/\/www.khanacademy.org\/science\/biology\/gene-expression-central-dogma\/translation-polypeptides\/a\/trna-and-ribosomes\">transfer RNAs<\/a><span style=\"font-size: 1em\">\u00a0and\u00a0<\/span><a class=\"_8gcxk83\" style=\"font-size: 1em\" href=\"https:\/\/www.khanacademy.org\/science\/biology\/gene-expression-central-dogma\/translation-polypeptides\/a\/trna-and-ribosomes\">ribosomal RNAs\u00a0<\/a><span style=\"font-size: 1em\">that play roles in translation.\u00a0<\/span><\/div>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">\r\n<div class=\"perseus-widget-container widget-nohighlight widget-inline\">\r\n<div class=\"_167wsvl\">\r\n<div><\/div>\r\n<div class=\"_36rlri\"><span style=\"color: #077fab;font-size: 1.15em;font-weight: 600\">How does the DNA sequence of a gene specify a particular protein?<\/span><\/div>\r\n<div><\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<div class=\"clearfix\">\r\n<div class=\"perseus-renderer perseus-renderer-responsive\">\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">Many genes provide instructions for building polypeptides. How, exactly, does DNA direct the construction of a polypeptide? This process involves two major steps: transcription and translation.<\/div>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<ul>\r\n \t<li>In\u00a0<strong>transcription<\/strong>, the DNA sequence of a gene is copied to make an RNA molecule. This step is called\u00a0<em>transcription<\/em>\u00a0because it involves rewriting, or transcribing, the DNA sequence in a similar RNA \"alphabet.\" In\u00a0<a class=\"_8gcxk83\" href=\"https:\/\/www.khanacademy.org\/science\/biology\/structure-of-a-cell\/prokaryotic-and-eukaryotic-cells\/v\/prokaryotic-and-eukaryotic-cells\">eukaryotes<\/a>, the RNA molecule must undergo processing to become a mature\u00a0<strong>messenger RNA<\/strong>\u00a0(<strong>mRNA<\/strong>).<\/li>\r\n<\/ul>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<ul>\r\n \t<li>In\u00a0<strong>translation<\/strong>, the sequence of the mRNA is decoded to specify the amino acid sequence of a polypeptide. The name\u00a0<em>translation<\/em>\u00a0reflects that the nucleotide sequence of the mRNA sequence must be translated into the completely different \"language\" of amino acids.<\/li>\r\n<\/ul>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">\r\n<div class=\"perseus-widget-container widget-nohighlight widget-block\">\r\n<div class=\"perseus-image-widget\">\r\n<div class=\"fixed-to-responsive zoomable svg-image\">\r\n<div><\/div>\r\n<img src=\"https:\/\/cdn.kastatic.org\/ka-perseus-images\/2b597889d05bc601803a3b4d9ec5ccd5e7b8d3af.png\" alt=\"Simplified schematic of central dogma, showing the sequences of the molecules involved. The two strands of DNA have the following sequences: 5'-ATGATCTCGTAA-3' 3'-TACTAGAGCATT-5' Transcription of one of the strands of DNA produces an mRNA that nearly matches the other strand of DNA in sequence. However, due to a biochemical difference between DNA and RNA, the Ts of DNA are replaced with Us in the mRNA. The mRNA sequence is: 5'-AUGAUCUCGUAA-5' Translation involves reading the mRNA nucleotides in groups of three; each group specifies an amino acid (or provides a stop signal indicating that translation is finished). 3'-AUG AUC UCG UAA-5' AUG $\\rightarrow$ Methionine AUC $\\rightarrow$ Isoleucine UCG $\\rightarrow$ Serine UAA $\\rightarrow$ &quot;Stop&quot; Polypeptide sequence: (N-terminus) Methionine-Isoleucine-Serine (C-terminus) \" \/>\r\n\r\n<\/div>\r\n<div class=\"perseus-renderer perseus-renderer-responsive\">\r\n<h1 class=\"paragraph\"><span style=\"color: #077fab;font-size: 1.15em;font-weight: 600\">Transcription<\/span><\/h1>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<div class=\"clearfix\">\r\n<div class=\"perseus-renderer perseus-renderer-responsive\">\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">In\u00a0<a class=\"_8gcxk83\" href=\"https:\/\/www.khanacademy.org\/science\/biology\/gene-expression-central-dogma\/transcription-of-dna-into-rna\/a\/overview-of-transcription\">transcription<\/a>, one strand of the DNA that makes up a gene, called the\u00a0<strong>non-coding strand<\/strong>, acts as a template for the synthesis of a matching (complementary) RNA strand by an enzyme called RNA polymerase. This RNA strand is the\u00a0<strong>primary transcript<\/strong>.<\/div>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">\r\n<div class=\"perseus-widget-container widget-nohighlight widget-block\">\r\n<div class=\"perseus-image-widget\">\r\n<div class=\"fixed-to-responsive zoomable svg-image\">\r\n<div><\/div>\r\n<img src=\"https:\/\/cdn.kastatic.org\/ka-perseus-images\/55e45fb61bd718907a9feee5987d04c0c2b055c3.png\" alt=\"The two strands of DNA have the following sequences: 5'-ATGATCTCGTAA-3' 3'-TACTAGAGCATT-5' The DNA opens up to form a bubble, and the lower strand serves as a template for the synthesis of a complementary RNA strand. This strand is called the template strand. Transcription of the template strand produces an mRNA that nearly matches the other strand (coding strand) of DNA in sequence. However, due to a biochemical difference between DNA and RNA, the Ts of DNA are replaced with Us in the mRNA. The mRNA sequence is: 5'-AUGAUCUCGUAA-5' \" \/>\r\n\r\n<\/div>\r\n<div class=\"perseus-renderer perseus-renderer-responsive\">\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">\r\n<div class=\"clearfix\">\r\n<div class=\"perseus-renderer perseus-renderer-responsive\">\r\n<div class=\"paragraph\">\r\n<h3>RNA polymerase<\/h3>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">The main enzyme involved in transcription is\u00a0<strong>RNA polymerase<\/strong>, which uses a single-stranded DNA template to synthesize a complementary strand of RNA. Specifically, RNA polymerase builds an RNA strand, adding each new nucleotide to the strand.<\/div>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">\r\n<div class=\"perseus-widget-container widget-nohighlight widget-block\">\r\n<div class=\"perseus-image-widget\">\r\n<div class=\"fixed-to-responsive zoomable svg-image\">\r\n<div><\/div>\r\n<img src=\"https:\/\/cdn.kastatic.org\/ka-perseus-images\/1e40590670cfdc967a5fe54d2e204df30e761232.png\" alt=\"RNA polymerase synthesizes an RNA strand complementary to a template DNA strand. It synthesizes the RNA strand in the 5' to 3' direction, while reading the template DNA strand in the 3' to 5' direction. The template DNA strand and RNA strand are antiparallel. RNA transcript: 5'-UGGUAGU...-3' (dots indicate where nucleotides are still being added at 3' end) DNA template: 3'-ACCATCAGTC-5'\" \/>\r\n\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<div class=\"clearfix\">\r\n<div class=\"perseus-renderer perseus-renderer-responsive\">\r\n<div class=\"paragraph\">\r\n<h3>Stages of transcription<\/h3>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">Transcription of a gene takes place in three stages: initiation, elongation, and termination. Here, we will briefly see how these steps happen.<\/div>\r\n<div><\/div>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<ol start=\"1\">\r\n \t<li>\r\n<div class=\"paragraph\"><strong>Initiation.<\/strong>\u00a0RNA polymerase binds to a sequence of DNA called the\u00a0<strong>promoter<\/strong>, found near the beginning of a gene. Each gene has its own promoter. Once bound, RNA polymerase separates the DNA strands, providing the single-stranded template needed for transcription.<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"perseus-widget-container widget-nohighlight widget-block\">\r\n<div class=\"perseus-image-widget\">\r\n<div class=\"fixed-to-responsive zoomable svg-image\">\r\n<div><\/div>\r\n<img src=\"https:\/\/cdn.kastatic.org\/ka-perseus-images\/22a15818999b09a1ba58a9abaadfe1045c93d2f4.png\" alt=\"The promoter region comes before (and slightly overlaps with) the transcribed region whose transcription it specifies. It contains recognition sites for RNA polymerase or its helper proteins to bind to. The DNA opens up in the promoter region so that RNA polymerase can begin transcription.\" \/>\r\n\r\n<\/div>\r\n<div class=\"perseus-renderer perseus-renderer-responsive\">\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">The promoter region comes before (and slightly overlaps with) the transcribed region whose transcription it specifies. It contains recognition sites for RNA polymerase or its helper proteins to bind to. The DNA opens up in the promoter region so that RNA polymerase can begin transcription.<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div><\/li>\r\n \t<li>\r\n<div class=\"paragraph\">\r\n\r\n<strong>Elongation.<\/strong>\u00a0One strand of DNA, the\u00a0<strong>template strand<\/strong>, acts as a template for RNA polymerase. As it \"reads\" this template one base at a time, the polymerase builds an RNA molecule out of complementary nucleotides, making a chain. The RNA transcript carries the same information as the non-template (<strong>coding<\/strong>) strand of DNA, but it contains the base uracil (U) instead of thymine (T).\r\n\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"perseus-widget-container widget-nohighlight widget-block\"><\/div>\r\n<\/div><\/li>\r\n \t<li>\r\n<div class=\"paragraph\"><strong>Termination.<\/strong>\u00a0Sequences called\u00a0<strong>terminators<\/strong>\u00a0signal that the RNA transcript is complete. Once they are transcribed, they cause the transcript to be released from the RNA polymerase. An example of a termination mechanism involving formation of a hairpin in the RNA is shown below.<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"perseus-widget-container widget-nohighlight widget-block\">\r\n<div class=\"perseus-image-widget\">\r\n<div class=\"fixed-to-responsive zoomable svg-image\">\r\n<div><\/div>\r\n<img src=\"https:\/\/cdn.kastatic.org\/ka-perseus-images\/4ffa46e26f38c8f8f1cbeccfec11781840f5a58d.png\" alt=\"The terminator DNA encodes a region of RNA that forms a hairpin structure followed by a string of U nucleotides. The hairpin structure in the transcript causes the RNA polymerase to stall. The U nucleotides that come after the hairpin form weak bonds with the A nucleotides of the DNA template, allowing the transcript to separate from the template and ending transcription.\" \/>\r\n\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div><\/li>\r\n<\/ol>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<h1>Translation<\/h1>\r\n<div class=\"clearfix\">\r\n<div class=\"perseus-renderer perseus-renderer-responsive\">\r\n<div class=\"paragraph\">\r\n<h3>The genetic code<\/h3>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">During translation, a cell \u201creads\u201d the information in a messenger RNA (mRNA) and uses it to build a protein. Actually, to be a little more technical, an mRNA doesn\u2019t always encode\u2014provide instructions for\u2014a whole protein. Instead, what we can confidently say is that it always encodes a\u00a0<strong>polypeptide<\/strong>, or chain of amino acids.<\/div>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">\r\n<div class=\"perseus-widget-container widget-nohighlight widget-inline\">\r\n<div class=\"_167wsvl\">\r\n<div class=\"_z5b02x\">\r\n<div class=\"perseus-renderer perseus-renderer-responsive\">\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\"><\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">\r\n<div class=\"perseus-widget-container widget-nohighlight widget-block\">\r\n<div class=\"perseus-image-widget\">\r\n<div class=\"fixed-to-responsive svg-image\"><img class=\"alignnone\" title=\"Image credit: &quot;The genetic code,&quot; by OpenStax College, Biology (CC BY 3.0)._\" src=\"https:\/\/cdn.kastatic.org\/ka-perseus-images\/f5de6355003ee322782b26404ef0733a1d1a61b0.png\" alt=\"Genetic code table. Each three-letter sequence of mRNA nucleotides corresponds to a specific amino acid, or to a stop codon. UGA, UAA, and UAG are stop codons. AUG is the codon for methionine, and is also the start codon.\" width=\"552\" height=\"473\" \/><\/div>\r\n<div class=\"perseus-renderer perseus-renderer-responsive\">\r\n<div class=\"paragraph\">\r\n<div><\/div>\r\n<div class=\"paragraph\">Each three-letter sequence of mRNA nucleotides corresponds to a specific amino acid, or to a stop codon. UGA, UAA, and UAG are stop codons. AUG is the codon for methionine, and is also the start codon.\u00a0<span style=\"font-size: 1em\">In an mRNA, the instructions for building a polypeptide are RNA nucleotides (As, Us, Cs, and Gs) read in groups of three. These groups of three are called\u00a0<\/span><strong style=\"font-size: 1em\">codons<\/strong><span style=\"font-size: 1em\">.\u00a0<\/span><span style=\"font-size: 1em\">\u00a0One codon, AUG, specifies the amino acid methionine and also acts as a\u00a0<\/span><strong style=\"font-size: 1em\">start codon<\/strong><span style=\"font-size: 1em\">\u00a0to signal the start of protein construction.\u00a0<\/span><span style=\"font-size: 1em\">There are three more codons that do\u00a0<\/span><em style=\"font-size: 1em\">not<\/em><span style=\"font-size: 1em\">\u00a0specify amino acids. These\u00a0<\/span><strong style=\"font-size: 1em\">stop codons<\/strong><span style=\"font-size: 1em\">, UAA, UAG, and UGA, tell the cell when a polypeptide is complete. All together, this collection of codon-amino acid relationships is called the\u00a0<\/span><strong style=\"font-size: 1em\">genetic code<\/strong><span style=\"font-size: 1em\">, because it lets cells \u201cdecode\u201d an mRNA into a chain of amino acids.<\/span><\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">\r\n<div class=\"perseus-widget-container widget-nohighlight widget-block\">\r\n<div class=\"perseus-image-widget\">\r\n<div class=\"fixed-to-responsive svg-image\">\r\n<div><\/div>\r\n<img class=\"alignnone\" title=\"Image modified from &quot;RNA-codons-aminoacids,&quot; by Thomas Splettstoesser (CC BY-SA 4.0). The modified image is licensed under a CC BY-SA 4.0 license.\" src=\"https:\/\/cdn.kastatic.org\/ka-perseus-images\/0527b4c194c122403fe2cdd3f0cabb20c777e532.png\" alt=\"Each mRNA contains a series of codons (nucleotide triplets) that each specifies an amino acid. The correspondence between mRNA codons and amino acids is called the genetic code. 5' AUG - Methionine ACG - Threonine GAG - Glutamate CUU - Leucine CGG - Arginine AGC - Serine UAG - Stop 3'\" width=\"697\" height=\"128\" \/>\r\n\r\n<\/div>\r\n<div class=\"perseus-renderer perseus-renderer-responsive\">\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">Each mRNA contains a series of codons (nucleotide triplets) that each specifies an amino acid. The correspondence between mRNA codons and amino acids is called the genetic code.<\/div>\r\n<div><\/div>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<div><\/div>\r\n<div class=\"paragraph\">AUG - Methionine ACG - Threonine GAG - Glutamate CUU - Leucine CGG - Arginine AGC - Serine UAG - Stop<\/div>\r\n<\/div>\r\n<\/div>\r\n<div class=\"perseus-image-caption\">\r\n<div class=\"perseus-renderer perseus-renderer-responsive\">\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\"><\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<div class=\"clearfix\">\r\n<div class=\"perseus-renderer perseus-renderer-responsive\">\r\n<div class=\"paragraph\"><\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\"><span style=\"color: #6c64ad;font-size: 1em;font-weight: 600\">Transfer RNAs (tRNAs)<\/span><\/div>\r\n<div><\/div>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\"><strong>Transfer RNAs<\/strong>, or\u00a0<strong>tRNAs<\/strong>, are molecular \"bridges\" that connect mRNA codons to the amino acids they encode. One end of each tRNA has a sequence of three nucleotides called an\u00a0<strong>anticodon<\/strong>, which can bind to specific mRNA codons. The other end of the tRNA carries the amino acid specified by the codons.\u00a0<span style=\"font-size: 1em\">There are many different types of tRNAs. Each type reads one or a few codons and brings the right amino acid matching those codons.<\/span><\/div>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">\r\n<div class=\"perseus-widget-container widget-nohighlight widget-block\">\r\n<div class=\"perseus-image-widget\">\r\n<div class=\"fixed-to-responsive zoomable svg-image\">\r\n<div><\/div>\r\n<img class=\"alignnone\" title=\"mage modified from &quot;Translation: Figure 3,&quot; by OpenStax College, Biology (CC BY 4.0).\" src=\"https:\/\/cdn.kastatic.org\/ka-perseus-images\/5882ce9e9f8ba89e8a6190f66935b6f9814859c4.png\" alt=\"Ribosomes are composed of a small and large subunit and have three sites where tRNAs can bind to an mRNA (the A, P, and E sites). Each tRNA vcarries a specific amino acid and binds to an mRNA codon that is complementary to its anticodon.\" width=\"3342\" height=\"1433\" \/>\r\n\r\n<\/div>\r\n<div class=\"perseus-renderer perseus-renderer-responsive\">\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">Ribosomes are composed of a small and large subunit and have three sites where tRNAs can bind to an mRNA (the A, P, and E sites). Each tRNA vcarries a specific amino acid and binds to an mRNA codon that is complementary to its anticodon.<\/div>\r\n<\/div>\r\n<\/div>\r\n<div class=\"perseus-image-caption\">\r\n<div class=\"perseus-renderer perseus-renderer-responsive\">\r\n<div class=\"paragraph\">\r\n<div><\/div>\r\n<div class=\"paragraph\"><span style=\"color: #6c64ad;font-size: 1em;font-weight: 600\">Ribosomes<\/span><\/div>\r\n<div><\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\"><strong>Ribosomes<\/strong>\u00a0are the structures where polypeptides (proteins) are built. They are made up of protein and RNA (<strong>ribosomal RNA<\/strong>, or\u00a0<strong>rRNA<\/strong>). Each ribosome has two subunits, a large one and a small one, which come together around an mRNA\u2014kind of like the two halves of a hamburger bun coming together around the patty.\u00a0<span style=\"font-size: 1em\">The ribosome provides a set of handy slots where tRNAs can find their matching codons on the mRNA template and deliver their amino acids. These slots are called the A, P, and E sites. Not only that, but the ribosome also acts as an enzyme, catalyzing the chemical reaction that links amino acids together to make a chain.<\/span><\/div>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<div><\/div>\r\n<div class=\"paragraph\"><span style=\"color: #077fab;font-size: 1.15em;font-weight: 600\">Steps of translation<\/span><\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<div class=\"clearfix\">\r\n<div class=\"perseus-renderer perseus-renderer-responsive\">\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">Your cells are making new proteins every second of the day. And each of those proteins must contain the right set of amino acids, linked together in just the right order. That may sound like a challenging task, but luckily, your cells (along with those of other animals, plants, and bacteria) are up to the job.<\/div>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">To see how cells make proteins, let's divide translation into three stages: initiation (starting off), elongation (adding on to the protein chain), and termination (finishing up).<\/div>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<h3>Getting started: Initiation<\/h3>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">In\u00a0<strong>initiation<\/strong>, the ribosome assembles around the mRNA to be read and the first tRNA (carrying the amino acid methionine, which matches the start codon, AUG). This setup, called the initiation complex, is needed in order for translation to get started.<\/div>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<h3>Extending the chain: Elongation<\/h3>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\"><strong>Elongation<\/strong>\u00a0is the stage where the amino acid chain gets\u00a0<strong>long<\/strong>er. In elongation, the mRNA is read one codon at a time, and the amino acid matching each codon is added to a growing protein chain.<\/div>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">Each time a new codon is exposed:<\/div>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<ul>\r\n \t<li>\r\n<div class=\"paragraph\">A matching tRNA binds to the codon<\/div><\/li>\r\n \t<li>\r\n<div class=\"paragraph\">The existing amino acid chain (polypeptide) is linked onto the amino acid of the tRNA via a chemical reaction<\/div><\/li>\r\n \t<li>\r\n<div class=\"paragraph\">The mRNA is shifted one codon over in the ribosome, exposing a new codon for reading<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"perseus-widget-container widget-nohighlight widget-block\">\r\n<div class=\"perseus-image-widget\">\r\n<div class=\"fixed-to-responsive zoomable svg-image\">\r\n<div><\/div>\r\n<img class=\"alignnone\" title=\"Image based on similar diagram in Reece et al.^2 2\" src=\"https:\/\/cdn.kastatic.org\/ka-perseus-images\/b526aaa21fea30cc9e3b1cb7da6d5ea6ee6520b3.png\" alt=\"Elongation has three stages: 1) The anticodon of an incoming tRNA pairs with the mRNA codon exposed in the A site. 2) A peptide bond is formed between the new amino acid (in the A site) and the previously-added amino acid (in the P site), transferring the polypeptide from the P site to the A site. 3) The ribosome moves one codon down on the mRNA. The tRNA in the A site (carrying the polypeptide) shifts to the P site. The tRNA in the P site shifts to the E site and exits the ribosome.\" width=\"2900\" height=\"2550\" \/>\r\n\r\n<\/div>\r\n<div class=\"perseus-renderer perseus-renderer-responsive\">\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">Elongation has three stages:<\/div>\r\n<div><\/div>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">1) The anticodon of an incoming tRNA pairs with the mRNA codon exposed in the A site.<\/div>\r\n<div><\/div>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">2) A peptide bond is formed between the new amino acid (in the A site) and the previously-added amino acid (in the P site), transferring the polypeptide from the P site to the A site.<\/div>\r\n<div><\/div>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">3) The ribosome moves one codon down on the mRNA. The tRNA in the A site (carrying the polypeptide) shifts to the P site. The tRNA in the P site shifts to the E site and exits the ribosome.<\/div>\r\n<\/div>\r\n<\/div>\r\n<div class=\"perseus-image-caption\">\r\n<div class=\"perseus-renderer perseus-renderer-responsive\">\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\"><\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div><\/li>\r\n<\/ul>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">During elongation, tRNAs move through the A, P, and E sites of the ribosome, as shown above. This process repeats many times as new codons are read and new amino acids are added to the chain.<\/div>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\">For more details on the steps of elongation, see the\u00a0<a class=\"_8gcxk83\" href=\"https:\/\/www.khanacademy.org\/science\/biology\/gene-expression-central-dogma\/translation-polypeptides\/a\/the-stages-of-translation\">stages of translation<\/a>article.<\/div>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<h3>Finishing up: Termination<\/h3>\r\n<\/div>\r\n<div class=\"paragraph\">\r\n<div class=\"paragraph\"><strong>Termination<\/strong>\u00a0is the stage in which the finished polypeptide chain is released. It begins when a stop codon (UAG, UAA, or UGA) enters the ribosome, triggering a series of events that separate the chain from its tRNA and allow it to drift out of the ribosome.\u00a0<span style=\"font-size: 1em\">After termination, the polypeptide may still need to fold into the right 3D shape, undergo processing (such as the removal of amino acids), get shipped to the\u00a0<\/span><a class=\"_8gcxk83\" style=\"font-size: 1em\" href=\"https:\/\/www.khanacademy.org\/science\/biology\/gene-expression-central-dogma\/translation-polypeptides\/a\/protein-targeting-and-traffic\">right place in the cell<\/a><span style=\"font-size: 1em\">, or combine with other polypeptides before it can do its job as a functional protein.<\/span><\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<div class=\"paragraph\"><\/div>\r\n<\/div>\r\n<\/div>","rendered":"<div class=\"clearfix\">\n<div class=\"perseus-renderer perseus-renderer-responsive\">\n<div class=\"paragraph\">\n<h2>Genes specify functional products (such as proteins)<\/h2>\n<\/div>\n<div class=\"paragraph\">\n<div class=\"paragraph\">A DNA molecule is divided up into functional units called\u00a0<strong>genes<\/strong>. Each gene provides instructions for a functional product, that is, a molecule needed to perform a job in the cell. In many cases, the functional product of a gene is a protein. For example, Mendel&#8217;s flower color gene provides instructions for a protein that helps make colored molecules (pigments) in flower petals.<\/div>\n<\/div>\n<div class=\"paragraph\">\n<div class=\"paragraph\">\n<div class=\"perseus-widget-container widget-nohighlight widget-block\">\n<div class=\"perseus-image-widget\">\n<div class=\"fixed-to-responsive zoomable svg-image\">\n<div><\/div>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone\" title=\"Image based on experimental data reported by Hellens et al.^1 1 start superscript, 1, end superscript and on similar figure in Reece et al.^2 2 start superscript, 2, end superscript.\" src=\"https:\/\/cdn.kastatic.org\/ka-perseus-images\/53b7ece60303244264411d03bfbe55d36312b64e.png\" alt=\"Diagram of how a gene can dictate a phenotype (observable feature) of an organism. The flower color gene that Mendel studied consists of a stretch of DNA found on a chromosome. The DNA has a particular sequence; part of it, shown in this diagram, is 5'-GTAAATCG-3' (upper strand), paired with the complementary sequence 3'-CATTTAGC-5' (lower strand). The DNA of the gene specifies production of a protein that helps make pigments. When the protein is present and functional, pigments are produced, and the flowers of a plant have a purple color.\" width=\"2100\" height=\"404\" \/><\/p>\n<\/div>\n<div class=\"perseus-renderer perseus-renderer-responsive\">\n<div class=\"paragraph\">\n<div class=\"paragraph\">The flower color gene that Mendel studied consists of a stretch of DNA found on a chromosome. The DNA has a particular sequence; part of it, shown in this diagram, is GTAAATCG (upper strand), paired with the complementary sequence CATTTAGC (lower strand). The DNA of the gene specifies production of a protein that helps make pigments. When the protein is present and functional, pigments are produced, and the flowers of a plant have a purple color.<\/div>\n<div><\/div>\n<\/div>\n<\/div>\n<div class=\"perseus-image-caption\">\n<div class=\"perseus-renderer perseus-renderer-responsive\">\n<div class=\"paragraph\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"paragraph\">\n<div class=\"paragraph\">The functional products of most known genes are proteins, or, more accurately, polypeptides.\u00a0<strong>Polypeptide<\/strong>\u00a0is just another word for a chain of amino acids. Although many proteins consist of a single polypeptide, some are made up of multiple polypeptides. Genes that specify polypeptides are called\u00a0<strong>protein-coding<\/strong>\u00a0genes.\u00a0<span style=\"font-size: 1em\">Not all genes specify polypeptides. Instead, some provide instructions to build functional RNA molecules, such as the\u00a0<\/span><a class=\"_8gcxk83\" style=\"font-size: 1em\" href=\"https:\/\/www.khanacademy.org\/science\/biology\/gene-expression-central-dogma\/translation-polypeptides\/a\/trna-and-ribosomes\">transfer RNAs<\/a><span style=\"font-size: 1em\">\u00a0and\u00a0<\/span><a class=\"_8gcxk83\" style=\"font-size: 1em\" href=\"https:\/\/www.khanacademy.org\/science\/biology\/gene-expression-central-dogma\/translation-polypeptides\/a\/trna-and-ribosomes\">ribosomal RNAs\u00a0<\/a><span style=\"font-size: 1em\">that play roles in translation.\u00a0<\/span><\/div>\n<\/div>\n<div class=\"paragraph\">\n<div class=\"paragraph\">\n<div class=\"perseus-widget-container widget-nohighlight widget-inline\">\n<div class=\"_167wsvl\">\n<div><\/div>\n<div class=\"_36rlri\"><span style=\"color: #077fab;font-size: 1.15em;font-weight: 600\">How does the DNA sequence of a gene specify a particular protein?<\/span><\/div>\n<div><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"clearfix\">\n<div class=\"perseus-renderer perseus-renderer-responsive\">\n<div class=\"paragraph\">\n<div class=\"paragraph\">Many genes provide instructions for building polypeptides. How, exactly, does DNA direct the construction of a polypeptide? This process involves two major steps: transcription and translation.<\/div>\n<\/div>\n<div class=\"paragraph\">\n<ul>\n<li>In\u00a0<strong>transcription<\/strong>, the DNA sequence of a gene is copied to make an RNA molecule. This step is called\u00a0<em>transcription<\/em>\u00a0because it involves rewriting, or transcribing, the DNA sequence in a similar RNA &#8220;alphabet.&#8221; In\u00a0<a class=\"_8gcxk83\" href=\"https:\/\/www.khanacademy.org\/science\/biology\/structure-of-a-cell\/prokaryotic-and-eukaryotic-cells\/v\/prokaryotic-and-eukaryotic-cells\">eukaryotes<\/a>, the RNA molecule must undergo processing to become a mature\u00a0<strong>messenger RNA<\/strong>\u00a0(<strong>mRNA<\/strong>).<\/li>\n<\/ul>\n<\/div>\n<div class=\"paragraph\">\n<ul>\n<li>In\u00a0<strong>translation<\/strong>, the sequence of the mRNA is decoded to specify the amino acid sequence of a polypeptide. The name\u00a0<em>translation<\/em>\u00a0reflects that the nucleotide sequence of the mRNA sequence must be translated into the completely different &#8220;language&#8221; of amino acids.<\/li>\n<\/ul>\n<\/div>\n<div class=\"paragraph\">\n<div class=\"paragraph\">\n<div class=\"perseus-widget-container widget-nohighlight widget-block\">\n<div class=\"perseus-image-widget\">\n<div class=\"fixed-to-responsive zoomable svg-image\">\n<div><\/div>\n<p><img decoding=\"async\" src=\"https:\/\/cdn.kastatic.org\/ka-perseus-images\/2b597889d05bc601803a3b4d9ec5ccd5e7b8d3af.png\" alt=\"Simplified schematic of central dogma, showing the sequences of the molecules involved. The two strands of DNA have the following sequences: 5'-ATGATCTCGTAA-3' 3'-TACTAGAGCATT-5' Transcription of one of the strands of DNA produces an mRNA that nearly matches the other strand of DNA in sequence. However, due to a biochemical difference between DNA and RNA, the Ts of DNA are replaced with Us in the mRNA. The mRNA sequence is: 5'-AUGAUCUCGUAA-5' Translation involves reading the mRNA nucleotides in groups of three; each group specifies an amino acid (or provides a stop signal indicating that translation is finished). 3'-AUG AUC UCG UAA-5' AUG $\\rightarrow$ Methionine AUC $\\rightarrow$ Isoleucine UCG $\\rightarrow$ Serine UAA $\\rightarrow$ &quot;Stop&quot; Polypeptide sequence: (N-terminus) Methionine-Isoleucine-Serine (C-terminus)\" \/><\/p>\n<\/div>\n<div class=\"perseus-renderer perseus-renderer-responsive\">\n<h1 class=\"paragraph\"><span style=\"color: #077fab;font-size: 1.15em;font-weight: 600\">Transcription<\/span><\/h1>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"clearfix\">\n<div class=\"perseus-renderer perseus-renderer-responsive\">\n<div class=\"paragraph\">\n<div class=\"paragraph\">In\u00a0<a class=\"_8gcxk83\" href=\"https:\/\/www.khanacademy.org\/science\/biology\/gene-expression-central-dogma\/transcription-of-dna-into-rna\/a\/overview-of-transcription\">transcription<\/a>, one strand of the DNA that makes up a gene, called the\u00a0<strong>non-coding strand<\/strong>, acts as a template for the synthesis of a matching (complementary) RNA strand by an enzyme called RNA polymerase. This RNA strand is the\u00a0<strong>primary transcript<\/strong>.<\/div>\n<\/div>\n<div class=\"paragraph\">\n<div class=\"paragraph\">\n<div class=\"perseus-widget-container widget-nohighlight widget-block\">\n<div class=\"perseus-image-widget\">\n<div class=\"fixed-to-responsive zoomable svg-image\">\n<div><\/div>\n<p><img decoding=\"async\" src=\"https:\/\/cdn.kastatic.org\/ka-perseus-images\/55e45fb61bd718907a9feee5987d04c0c2b055c3.png\" alt=\"The two strands of DNA have the following sequences: 5'-ATGATCTCGTAA-3' 3'-TACTAGAGCATT-5' The DNA opens up to form a bubble, and the lower strand serves as a template for the synthesis of a complementary RNA strand. This strand is called the template strand. Transcription of the template strand produces an mRNA that nearly matches the other strand (coding strand) of DNA in sequence. However, due to a biochemical difference between DNA and RNA, the Ts of DNA are replaced with Us in the mRNA. The mRNA sequence is: 5'-AUGAUCUCGUAA-5'\" \/><\/p>\n<\/div>\n<div class=\"perseus-renderer perseus-renderer-responsive\">\n<div class=\"paragraph\">\n<div class=\"paragraph\">\n<div class=\"clearfix\">\n<div class=\"perseus-renderer perseus-renderer-responsive\">\n<div class=\"paragraph\">\n<h3>RNA polymerase<\/h3>\n<\/div>\n<div class=\"paragraph\">\n<div class=\"paragraph\">The main enzyme involved in transcription is\u00a0<strong>RNA polymerase<\/strong>, which uses a single-stranded DNA template to synthesize a complementary strand of RNA. Specifically, RNA polymerase builds an RNA strand, adding each new nucleotide to the strand.<\/div>\n<\/div>\n<div class=\"paragraph\">\n<div class=\"paragraph\">\n<div class=\"perseus-widget-container widget-nohighlight widget-block\">\n<div class=\"perseus-image-widget\">\n<div class=\"fixed-to-responsive zoomable svg-image\">\n<div><\/div>\n<p><img decoding=\"async\" src=\"https:\/\/cdn.kastatic.org\/ka-perseus-images\/1e40590670cfdc967a5fe54d2e204df30e761232.png\" alt=\"RNA polymerase synthesizes an RNA strand complementary to a template DNA strand. It synthesizes the RNA strand in the 5' to 3' direction, while reading the template DNA strand in the 3' to 5' direction. The template DNA strand and RNA strand are antiparallel. RNA transcript: 5'-UGGUAGU...-3' (dots indicate where nucleotides are still being added at 3' end) DNA template: 3'-ACCATCAGTC-5'\" \/><\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"clearfix\">\n<div class=\"perseus-renderer perseus-renderer-responsive\">\n<div class=\"paragraph\">\n<h3>Stages of transcription<\/h3>\n<\/div>\n<div class=\"paragraph\">\n<div class=\"paragraph\">Transcription of a gene takes place in three stages: initiation, elongation, and termination. Here, we will briefly see how these steps happen.<\/div>\n<div><\/div>\n<\/div>\n<div class=\"paragraph\">\n<ol start=\"1\">\n<li>\n<div class=\"paragraph\"><strong>Initiation.<\/strong>\u00a0RNA polymerase binds to a sequence of DNA called the\u00a0<strong>promoter<\/strong>, found near the beginning of a gene. Each gene has its own promoter. Once bound, RNA polymerase separates the DNA strands, providing the single-stranded template needed for transcription.<\/div>\n<div class=\"paragraph\">\n<div class=\"perseus-widget-container widget-nohighlight widget-block\">\n<div class=\"perseus-image-widget\">\n<div class=\"fixed-to-responsive zoomable svg-image\">\n<div><\/div>\n<p><img decoding=\"async\" src=\"https:\/\/cdn.kastatic.org\/ka-perseus-images\/22a15818999b09a1ba58a9abaadfe1045c93d2f4.png\" alt=\"The promoter region comes before (and slightly overlaps with) the transcribed region whose transcription it specifies. It contains recognition sites for RNA polymerase or its helper proteins to bind to. The DNA opens up in the promoter region so that RNA polymerase can begin transcription.\" \/><\/p>\n<\/div>\n<div class=\"perseus-renderer perseus-renderer-responsive\">\n<div class=\"paragraph\">\n<div class=\"paragraph\">The promoter region comes before (and slightly overlaps with) the transcribed region whose transcription it specifies. It contains recognition sites for RNA polymerase or its helper proteins to bind to. The DNA opens up in the promoter region so that RNA polymerase can begin transcription.<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/li>\n<li>\n<div class=\"paragraph\">\n<p><strong>Elongation.<\/strong>\u00a0One strand of DNA, the\u00a0<strong>template strand<\/strong>, acts as a template for RNA polymerase. As it &#8220;reads&#8221; this template one base at a time, the polymerase builds an RNA molecule out of complementary nucleotides, making a chain. The RNA transcript carries the same information as the non-template (<strong>coding<\/strong>) strand of DNA, but it contains the base uracil (U) instead of thymine (T).<\/p>\n<\/div>\n<div class=\"paragraph\">\n<div class=\"perseus-widget-container widget-nohighlight widget-block\"><\/div>\n<\/div>\n<\/li>\n<li>\n<div class=\"paragraph\"><strong>Termination.<\/strong>\u00a0Sequences called\u00a0<strong>terminators<\/strong>\u00a0signal that the RNA transcript is complete. Once they are transcribed, they cause the transcript to be released from the RNA polymerase. An example of a termination mechanism involving formation of a hairpin in the RNA is shown below.<\/div>\n<div class=\"paragraph\">\n<div class=\"perseus-widget-container widget-nohighlight widget-block\">\n<div class=\"perseus-image-widget\">\n<div class=\"fixed-to-responsive zoomable svg-image\">\n<div><\/div>\n<p><img decoding=\"async\" src=\"https:\/\/cdn.kastatic.org\/ka-perseus-images\/4ffa46e26f38c8f8f1cbeccfec11781840f5a58d.png\" alt=\"The terminator DNA encodes a region of RNA that forms a hairpin structure followed by a string of U nucleotides. The hairpin structure in the transcript causes the RNA polymerase to stall. The U nucleotides that come after the hairpin form weak bonds with the A nucleotides of the DNA template, allowing the transcript to separate from the template and ending transcription.\" \/><\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/li>\n<\/ol>\n<\/div>\n<\/div>\n<\/div>\n<h1>Translation<\/h1>\n<div class=\"clearfix\">\n<div class=\"perseus-renderer perseus-renderer-responsive\">\n<div class=\"paragraph\">\n<h3>The genetic code<\/h3>\n<\/div>\n<div class=\"paragraph\">\n<div class=\"paragraph\">During translation, a cell \u201creads\u201d the information in a messenger RNA (mRNA) and uses it to build a protein. Actually, to be a little more technical, an mRNA doesn\u2019t always encode\u2014provide instructions for\u2014a whole protein. Instead, what we can confidently say is that it always encodes a\u00a0<strong>polypeptide<\/strong>, or chain of amino acids.<\/div>\n<\/div>\n<div class=\"paragraph\">\n<div class=\"paragraph\">\n<div class=\"perseus-widget-container widget-nohighlight widget-inline\">\n<div class=\"_167wsvl\">\n<div class=\"_z5b02x\">\n<div class=\"perseus-renderer perseus-renderer-responsive\">\n<div class=\"paragraph\">\n<div class=\"paragraph\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"paragraph\">\n<div class=\"paragraph\">\n<div class=\"perseus-widget-container widget-nohighlight widget-block\">\n<div class=\"perseus-image-widget\">\n<div class=\"fixed-to-responsive svg-image\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone\" title=\"Image credit: &quot;The genetic code,&quot; by OpenStax College, Biology (CC BY 3.0)._\" src=\"https:\/\/cdn.kastatic.org\/ka-perseus-images\/f5de6355003ee322782b26404ef0733a1d1a61b0.png\" alt=\"Genetic code table. Each three-letter sequence of mRNA nucleotides corresponds to a specific amino acid, or to a stop codon. UGA, UAA, and UAG are stop codons. AUG is the codon for methionine, and is also the start codon.\" width=\"552\" height=\"473\" \/><\/div>\n<div class=\"perseus-renderer perseus-renderer-responsive\">\n<div class=\"paragraph\">\n<div><\/div>\n<div class=\"paragraph\">Each three-letter sequence of mRNA nucleotides corresponds to a specific amino acid, or to a stop codon. UGA, UAA, and UAG are stop codons. AUG is the codon for methionine, and is also the start codon.\u00a0<span style=\"font-size: 1em\">In an mRNA, the instructions for building a polypeptide are RNA nucleotides (As, Us, Cs, and Gs) read in groups of three. These groups of three are called\u00a0<\/span><strong style=\"font-size: 1em\">codons<\/strong><span style=\"font-size: 1em\">.\u00a0<\/span><span style=\"font-size: 1em\">\u00a0One codon, AUG, specifies the amino acid methionine and also acts as a\u00a0<\/span><strong style=\"font-size: 1em\">start codon<\/strong><span style=\"font-size: 1em\">\u00a0to signal the start of protein construction.\u00a0<\/span><span style=\"font-size: 1em\">There are three more codons that do\u00a0<\/span><em style=\"font-size: 1em\">not<\/em><span style=\"font-size: 1em\">\u00a0specify amino acids. These\u00a0<\/span><strong style=\"font-size: 1em\">stop codons<\/strong><span style=\"font-size: 1em\">, UAA, UAG, and UGA, tell the cell when a polypeptide is complete. All together, this collection of codon-amino acid relationships is called the\u00a0<\/span><strong style=\"font-size: 1em\">genetic code<\/strong><span style=\"font-size: 1em\">, because it lets cells \u201cdecode\u201d an mRNA into a chain of amino acids.<\/span><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"paragraph\">\n<div class=\"paragraph\">\n<div class=\"perseus-widget-container widget-nohighlight widget-block\">\n<div class=\"perseus-image-widget\">\n<div class=\"fixed-to-responsive svg-image\">\n<div><\/div>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone\" title=\"Image modified from &quot;RNA-codons-aminoacids,&quot; by Thomas Splettstoesser (CC BY-SA 4.0). The modified image is licensed under a CC BY-SA 4.0 license.\" src=\"https:\/\/cdn.kastatic.org\/ka-perseus-images\/0527b4c194c122403fe2cdd3f0cabb20c777e532.png\" alt=\"Each mRNA contains a series of codons (nucleotide triplets) that each specifies an amino acid. The correspondence between mRNA codons and amino acids is called the genetic code. 5' AUG - Methionine ACG - Threonine GAG - Glutamate CUU - Leucine CGG - Arginine AGC - Serine UAG - Stop 3'\" width=\"697\" height=\"128\" \/><\/p>\n<\/div>\n<div class=\"perseus-renderer perseus-renderer-responsive\">\n<div class=\"paragraph\">\n<div class=\"paragraph\">Each mRNA contains a series of codons (nucleotide triplets) that each specifies an amino acid. The correspondence between mRNA codons and amino acids is called the genetic code.<\/div>\n<div><\/div>\n<\/div>\n<div class=\"paragraph\">\n<div><\/div>\n<div class=\"paragraph\">AUG &#8211; Methionine ACG &#8211; Threonine GAG &#8211; Glutamate CUU &#8211; Leucine CGG &#8211; Arginine AGC &#8211; Serine UAG &#8211; Stop<\/div>\n<\/div>\n<\/div>\n<div class=\"perseus-image-caption\">\n<div class=\"perseus-renderer perseus-renderer-responsive\">\n<div class=\"paragraph\">\n<div class=\"paragraph\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"clearfix\">\n<div class=\"perseus-renderer perseus-renderer-responsive\">\n<div class=\"paragraph\"><\/div>\n<div class=\"paragraph\">\n<div class=\"paragraph\"><span style=\"color: #6c64ad;font-size: 1em;font-weight: 600\">Transfer RNAs (tRNAs)<\/span><\/div>\n<div><\/div>\n<\/div>\n<div class=\"paragraph\">\n<div class=\"paragraph\"><strong>Transfer RNAs<\/strong>, or\u00a0<strong>tRNAs<\/strong>, are molecular &#8220;bridges&#8221; that connect mRNA codons to the amino acids they encode. One end of each tRNA has a sequence of three nucleotides called an\u00a0<strong>anticodon<\/strong>, which can bind to specific mRNA codons. The other end of the tRNA carries the amino acid specified by the codons.\u00a0<span style=\"font-size: 1em\">There are many different types of tRNAs. Each type reads one or a few codons and brings the right amino acid matching those codons.<\/span><\/div>\n<\/div>\n<div class=\"paragraph\">\n<div class=\"paragraph\">\n<div class=\"perseus-widget-container widget-nohighlight widget-block\">\n<div class=\"perseus-image-widget\">\n<div class=\"fixed-to-responsive zoomable svg-image\">\n<div><\/div>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone\" title=\"mage modified from &quot;Translation: Figure 3,&quot; by OpenStax College, Biology (CC BY 4.0).\" src=\"https:\/\/cdn.kastatic.org\/ka-perseus-images\/5882ce9e9f8ba89e8a6190f66935b6f9814859c4.png\" alt=\"Ribosomes are composed of a small and large subunit and have three sites where tRNAs can bind to an mRNA (the A, P, and E sites). Each tRNA vcarries a specific amino acid and binds to an mRNA codon that is complementary to its anticodon.\" width=\"3342\" height=\"1433\" \/><\/p>\n<\/div>\n<div class=\"perseus-renderer perseus-renderer-responsive\">\n<div class=\"paragraph\">\n<div class=\"paragraph\">Ribosomes are composed of a small and large subunit and have three sites where tRNAs can bind to an mRNA (the A, P, and E sites). Each tRNA vcarries a specific amino acid and binds to an mRNA codon that is complementary to its anticodon.<\/div>\n<\/div>\n<\/div>\n<div class=\"perseus-image-caption\">\n<div class=\"perseus-renderer perseus-renderer-responsive\">\n<div class=\"paragraph\">\n<div><\/div>\n<div class=\"paragraph\"><span style=\"color: #6c64ad;font-size: 1em;font-weight: 600\">Ribosomes<\/span><\/div>\n<div><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"paragraph\">\n<div class=\"paragraph\"><strong>Ribosomes<\/strong>\u00a0are the structures where polypeptides (proteins) are built. They are made up of protein and RNA (<strong>ribosomal RNA<\/strong>, or\u00a0<strong>rRNA<\/strong>). Each ribosome has two subunits, a large one and a small one, which come together around an mRNA\u2014kind of like the two halves of a hamburger bun coming together around the patty.\u00a0<span style=\"font-size: 1em\">The ribosome provides a set of handy slots where tRNAs can find their matching codons on the mRNA template and deliver their amino acids. These slots are called the A, P, and E sites. Not only that, but the ribosome also acts as an enzyme, catalyzing the chemical reaction that links amino acids together to make a chain.<\/span><\/div>\n<\/div>\n<div class=\"paragraph\">\n<div><\/div>\n<div class=\"paragraph\"><span style=\"color: #077fab;font-size: 1.15em;font-weight: 600\">Steps of translation<\/span><\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"clearfix\">\n<div class=\"perseus-renderer perseus-renderer-responsive\">\n<div class=\"paragraph\">\n<div class=\"paragraph\">Your cells are making new proteins every second of the day. And each of those proteins must contain the right set of amino acids, linked together in just the right order. That may sound like a challenging task, but luckily, your cells (along with those of other animals, plants, and bacteria) are up to the job.<\/div>\n<\/div>\n<div class=\"paragraph\">\n<div class=\"paragraph\">To see how cells make proteins, let&#8217;s divide translation into three stages: initiation (starting off), elongation (adding on to the protein chain), and termination (finishing up).<\/div>\n<\/div>\n<div class=\"paragraph\">\n<h3>Getting started: Initiation<\/h3>\n<\/div>\n<div class=\"paragraph\">\n<div class=\"paragraph\">In\u00a0<strong>initiation<\/strong>, the ribosome assembles around the mRNA to be read and the first tRNA (carrying the amino acid methionine, which matches the start codon, AUG). This setup, called the initiation complex, is needed in order for translation to get started.<\/div>\n<\/div>\n<div class=\"paragraph\">\n<h3>Extending the chain: Elongation<\/h3>\n<\/div>\n<div class=\"paragraph\">\n<div class=\"paragraph\"><strong>Elongation<\/strong>\u00a0is the stage where the amino acid chain gets\u00a0<strong>long<\/strong>er. In elongation, the mRNA is read one codon at a time, and the amino acid matching each codon is added to a growing protein chain.<\/div>\n<\/div>\n<div class=\"paragraph\">\n<div class=\"paragraph\">Each time a new codon is exposed:<\/div>\n<\/div>\n<div class=\"paragraph\">\n<ul>\n<li>\n<div class=\"paragraph\">A matching tRNA binds to the codon<\/div>\n<\/li>\n<li>\n<div class=\"paragraph\">The existing amino acid chain (polypeptide) is linked onto the amino acid of the tRNA via a chemical reaction<\/div>\n<\/li>\n<li>\n<div class=\"paragraph\">The mRNA is shifted one codon over in the ribosome, exposing a new codon for reading<\/div>\n<div class=\"paragraph\">\n<div class=\"perseus-widget-container widget-nohighlight widget-block\">\n<div class=\"perseus-image-widget\">\n<div class=\"fixed-to-responsive zoomable svg-image\">\n<div><\/div>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone\" title=\"Image based on similar diagram in Reece et al.^2 2\" src=\"https:\/\/cdn.kastatic.org\/ka-perseus-images\/b526aaa21fea30cc9e3b1cb7da6d5ea6ee6520b3.png\" alt=\"Elongation has three stages: 1) The anticodon of an incoming tRNA pairs with the mRNA codon exposed in the A site. 2) A peptide bond is formed between the new amino acid (in the A site) and the previously-added amino acid (in the P site), transferring the polypeptide from the P site to the A site. 3) The ribosome moves one codon down on the mRNA. The tRNA in the A site (carrying the polypeptide) shifts to the P site. The tRNA in the P site shifts to the E site and exits the ribosome.\" width=\"2900\" height=\"2550\" \/><\/p>\n<\/div>\n<div class=\"perseus-renderer perseus-renderer-responsive\">\n<div class=\"paragraph\">\n<div class=\"paragraph\">Elongation has three stages:<\/div>\n<div><\/div>\n<\/div>\n<div class=\"paragraph\">\n<div class=\"paragraph\">1) The anticodon of an incoming tRNA pairs with the mRNA codon exposed in the A site.<\/div>\n<div><\/div>\n<\/div>\n<div class=\"paragraph\">\n<div class=\"paragraph\">2) A peptide bond is formed between the new amino acid (in the A site) and the previously-added amino acid (in the P site), transferring the polypeptide from the P site to the A site.<\/div>\n<div><\/div>\n<\/div>\n<div class=\"paragraph\">\n<div class=\"paragraph\">3) The ribosome moves one codon down on the mRNA. The tRNA in the A site (carrying the polypeptide) shifts to the P site. The tRNA in the P site shifts to the E site and exits the ribosome.<\/div>\n<\/div>\n<\/div>\n<div class=\"perseus-image-caption\">\n<div class=\"perseus-renderer perseus-renderer-responsive\">\n<div class=\"paragraph\">\n<div class=\"paragraph\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/li>\n<\/ul>\n<\/div>\n<div class=\"paragraph\">\n<div class=\"paragraph\">During elongation, tRNAs move through the A, P, and E sites of the ribosome, as shown above. This process repeats many times as new codons are read and new amino acids are added to the chain.<\/div>\n<\/div>\n<div class=\"paragraph\">\n<div class=\"paragraph\">For more details on the steps of elongation, see the\u00a0<a class=\"_8gcxk83\" href=\"https:\/\/www.khanacademy.org\/science\/biology\/gene-expression-central-dogma\/translation-polypeptides\/a\/the-stages-of-translation\">stages of translation<\/a>article.<\/div>\n<\/div>\n<div class=\"paragraph\">\n<h3>Finishing up: Termination<\/h3>\n<\/div>\n<div class=\"paragraph\">\n<div class=\"paragraph\"><strong>Termination<\/strong>\u00a0is the stage in which the finished polypeptide chain is released. It begins when a stop codon (UAG, UAA, or UGA) enters the ribosome, triggering a series of events that separate the chain from its tRNA and allow it to drift out of the ribosome.\u00a0<span style=\"font-size: 1em\">After termination, the polypeptide may still need to fold into the right 3D shape, undergo processing (such as the removal of amino acids), get shipped to the\u00a0<\/span><a class=\"_8gcxk83\" style=\"font-size: 1em\" href=\"https:\/\/www.khanacademy.org\/science\/biology\/gene-expression-central-dogma\/translation-polypeptides\/a\/protein-targeting-and-traffic\">right place in the cell<\/a><span style=\"font-size: 1em\">, or combine with other polypeptides before it can do its job as a functional protein.<\/span><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"paragraph\"><\/div>\n<\/div>\n<\/div>\n\n\t\t\t <section class=\"citations-section\" role=\"contentinfo\">\n\t\t\t <h3>Candela Citations<\/h3>\n\t\t\t\t\t <div>\n\t\t\t\t\t\t <div id=\"citation-list-4955\">\n\t\t\t\t\t\t\t <div class=\"licensing\"><div class=\"license-attribution-dropdown-subheading\">CC licensed content, Shared previously<\/div><ul class=\"citation-list\"><li><strong>Provided by<\/strong>: Khan Academy. <strong>Located at<\/strong>: <a target=\"_blank\" href=\"https:\/\/www.khanacademy.org\/science\/biology\/gene-expression-central-dogma\/translation-polypeptides\/a\/translation-overview\">https:\/\/www.khanacademy.org\/science\/biology\/gene-expression-central-dogma\/translation-polypeptides\/a\/translation-overview<\/a>. <strong>License<\/strong>: <em><a target=\"_blank\" rel=\"license\" href=\"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/\">CC BY-NC-SA: Attribution-NonCommercial-ShareAlike<\/a><\/em><\/li><\/ul><\/div>\n\t\t\t\t\t\t <\/div>\n\t\t\t\t\t <\/div>\n\t\t\t <\/section>","protected":false},"author":57729,"menu_order":5,"template":"","meta":{"_candela_citation":"[{\"type\":\"cc\",\"description\":\"\",\"author\":\"\",\"organization\":\"Khan Academy\",\"url\":\"https:\/\/www.khanacademy.org\/science\/biology\/gene-expression-central-dogma\/translation-polypeptides\/a\/translation-overview\",\"project\":\"\",\"license\":\"cc-by-nc-sa\",\"license_terms\":\"\"}]","CANDELA_OUTCOMES_GUID":"","pb_show_title":"on","pb_short_title":"","pb_subtitle":"","pb_authors":[],"pb_section_license":""},"chapter-type":[],"contributor":[],"license":[],"class_list":["post-4955","chapter","type-chapter","status-publish","hentry"],"part":4375,"_links":{"self":[{"href":"https:\/\/courses.lumenlearning.com\/suny-dutchess-ap1\/wp-json\/pressbooks\/v2\/chapters\/4955","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/courses.lumenlearning.com\/suny-dutchess-ap1\/wp-json\/pressbooks\/v2\/chapters"}],"about":[{"href":"https:\/\/courses.lumenlearning.com\/suny-dutchess-ap1\/wp-json\/wp\/v2\/types\/chapter"}],"author":[{"embeddable":true,"href":"https:\/\/courses.lumenlearning.com\/suny-dutchess-ap1\/wp-json\/wp\/v2\/users\/57729"}],"version-history":[{"count":9,"href":"https:\/\/courses.lumenlearning.com\/suny-dutchess-ap1\/wp-json\/pressbooks\/v2\/chapters\/4955\/revisions"}],"predecessor-version":[{"id":5984,"href":"https:\/\/courses.lumenlearning.com\/suny-dutchess-ap1\/wp-json\/pressbooks\/v2\/chapters\/4955\/revisions\/5984"}],"part":[{"href":"https:\/\/courses.lumenlearning.com\/suny-dutchess-ap1\/wp-json\/pressbooks\/v2\/parts\/4375"}],"metadata":[{"href":"https:\/\/courses.lumenlearning.com\/suny-dutchess-ap1\/wp-json\/pressbooks\/v2\/chapters\/4955\/metadata\/"}],"wp:attachment":[{"href":"https:\/\/courses.lumenlearning.com\/suny-dutchess-ap1\/wp-json\/wp\/v2\/media?parent=4955"}],"wp:term":[{"taxonomy":"chapter-type","embeddable":true,"href":"https:\/\/courses.lumenlearning.com\/suny-dutchess-ap1\/wp-json\/pressbooks\/v2\/chapter-type?post=4955"},{"taxonomy":"contributor","embeddable":true,"href":"https:\/\/courses.lumenlearning.com\/suny-dutchess-ap1\/wp-json\/wp\/v2\/contributor?post=4955"},{"taxonomy":"license","embeddable":true,"href":"https:\/\/courses.lumenlearning.com\/suny-dutchess-ap1\/wp-json\/wp\/v2\/license?post=4955"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}