{"id":87,"date":"2021-01-25T00:56:06","date_gmt":"2021-01-25T00:56:06","guid":{"rendered":"https:\/\/courses.lumenlearning.com\/suny-hccc-generalscience\/?post_type=chapter&#038;p=87"},"modified":"2021-01-25T00:56:06","modified_gmt":"2021-01-25T00:56:06","slug":"5-comparison-in-scientific-research","status":"publish","type":"chapter","link":"https:\/\/courses.lumenlearning.com\/suny-hccc-generalscience\/chapter\/5-comparison-in-scientific-research\/","title":{"raw":"5. Comparison in Scientific Research","rendered":"5. Comparison in Scientific Research"},"content":{"raw":"<div class=\"article-introduction\">\r\n\r\nnyone who has stared at a chimpanzee in a zoo (Figure 1) has probably wondered about the animal's similarity to humans. Chimps make facial expressions that resemble humans, use their hands in much the same way we do, are adept at using different objects as tools, and even laugh when they are tickled. It may not be surprising to learn then that when the first captured chimpanzees were brought to Europe in the 17<sup>th<\/sup> century, people were confused, labeling the animals \"pygmies\" and speculating that they were stunted versions of \"full-grown\" humans. A London physician named <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/Tyson%2C+Edward\/pop\">Edward Tyson<\/a> obtained a \"pygmie\" that had died of an infection shortly after arriving in London, and began a systematic study of the animal that cataloged the differences between chimpanzees and humans, thus helping to establish <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a> as a scientific <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/method\/pop\">method<\/a>.\r\n<figure><img src=\"https:\/\/www.visionlearning.com\/img\/library\/modules\/mid152\/Image\/VLObject-1987-030729120734.jpg\" alt=\"chimpanzee\" \/><figcaption>Figure 1: A chimpanzee <span class=\"credit\">image \u00a9 Corel Corporation<\/span><\/figcaption><\/figure>\r\n<\/div>\r\n<section id=\"toc_1\" class=\"article-section\">\r\n<h2>A brief history of comparative methods<\/h2>\r\nIn 1698, Tyson, a member of the Royal Society of London, began a detailed dissection of the \"pygmie\" he had obtained and published his findings in the 1699 work: <em>Orang-Outang, sive Homo Sylvestris: or, the Anatomy of a Pygmie Compared with that of a Monkey, an Ape, and a Man<\/em>. The title of the work further reflects the misconception that existed at the time \u2013 Tyson did not use the term Orang-Outang in its modern sense to refer to the orangutan; he used it in its literal translation from the Malay language as \"man of the woods,\" as that is how the chimps were viewed.\r\n\r\nTyson took great care in his dissection. He precisely measured and compared a number of anatomical <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/variable\/pop\">variables<\/a> such as brain size of the \"pygmie,\" ape, and human. He recorded his measurements of the \"pygmie,\" even down to the direction in which the animal's hair grew: \"The tendency of the Hair of all of the Body was downwards; but only from the Wrists to the Elbows 'twas upwards\" (Russell, 1967). Aided by William Cowper, Tyson made drawings of various anatomical structures, taking great care to accurately depict the dimensions of these structures so that they could be compared to those in humans (Figure 2). His systematic <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> study of the dimensions of anatomical structures in the chimp, ape, and human led him to state:\r\n<blockquote>in the Organization of abundance of its Parts, it more approached to the Structure of the same in Men: But where it differs from a Man, there it resembles plainly the Common Ape, more than any other Animal. (Russell, 1967)<\/blockquote>\r\nTyson's <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> studies proved exceptionally accurate and his <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a> was used by others, including <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/Huxley%2C+Thomas+Henry\/pop\">Thomas Henry Huxley<\/a> in <em>Evidence as to Man's Place in Nature<\/em> (1863) and <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/Darwin%2C+Charles\/pop\">Charles Darwin<\/a> in <em>The Descent of Man<\/em> (1871).\r\n<figure><img src=\"https:\/\/www.visionlearning.com\/img\/library\/modules\/mid152\/Image\/VLObject-3893-080924010924.jpg\" alt=\"pygmie and skeleton\" \/><figcaption>Figure 2: Edward Tyson's drawing of the external appearance of a \"pygmie\" (left) and the animal's skeleton (right) from <em>The Anatomy of a Pygmie Compared with that of a Monkey, an Ape, and a Man<\/em> from the second edition, London, printed for T. Osborne, 1751.<\/figcaption><\/figure>\r\nTyson's methodical and scientific approach to anatomical dissection contributed to the development of evolutionary <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/theory\/pop\">theory<\/a> and helped establish the field of <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> anatomy. Further, Tyson's work helps to highlight the importance of comparison as a scientific <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a> <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/method\/pop\">method<\/a>.\r\n\r\n<\/section><section id=\"toc_2\" class=\"article-section\">\r\n<h2>Comparison as a scientific research method<\/h2>\r\nComparative <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a> represents one approach in the spectrum of scientific research <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/method\/pop\">methods<\/a> and in some ways is a hybrid of other methods, drawing on aspects of both experimental science (see our <a href=\"https:\/\/www.visionlearning.com\/library\/module_viewer.php?mid=150\">Experimentation in Science<\/a> module) and descriptive research (see our <a href=\"https:\/\/www.visionlearning.com\/library\/module_viewer.php?mid=151\">Description in Science<\/a> module). Similar to experimentation, comparison seeks to decipher the relationship between two or more <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/variable\/pop\">variables<\/a> by documenting observed differences and similarities between two or more <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/subject\/pop\">subjects<\/a> or groups. In contrast to experimentation, the <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> researcher does not subject one of those groups to a <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/treatment\/pop\">treatment<\/a>, but rather observes a group that either by choice or circumstance has been subject to a treatment. Thus comparison involves <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/observation\/pop\">observation<\/a> in a more \"natural\" setting, not subject to experimental confines, and in this way evokes similarities with description.\r\n\r\nImportantly, the simple comparison of two <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/variable\/pop\">variables<\/a> or objects is not <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a>. Tyson's work would not have been considered scientific research if he had simply noted that \"pygmies\" looked like humans without measuring bone lengths and hair growth patterns. Instead, comparative research involves the systematic cataloging of the nature and\/or behavior of two or more variables, and the quantification of the relationship between them.\r\n<figure><a title=\"skeleton chimpanzee\" href=\"https:\/\/www.visionlearning.com\/img\/library\/large_images\/image_3894.jpg\"> <img src=\"https:\/\/www.visionlearning.com\/img\/library\/modules\/mid152\/Image\/VLObject-3894-080924010927.jpg\" alt=\"skeleton chimpanzee\" \/> <\/a><figcaption>Figure 3: Skeleton of the juvenile chimpanzee dissected by Edward Tyson, currently displayed at the Natural History Museum, London. <span class=\"credit\">image \u00a9 Peter Kaminski<\/span><\/figcaption><\/figure>\r\nWhile the choice of which <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a> <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/method\/pop\">method<\/a> to use is a personal decision based in part on the training of the researchers conducting the study, there are a number of scenarios in which <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> research would likely be the primary choice.\r\n<blockquote>\r\n<ul class=\"bulleted\">\r\n \t<li>The first scenario is one in which the scientist is not trying to measure a response to change, but rather he or she may be trying to understand the similarities and differences between two subjects. For example, Tyson was not observing a change in his \"pygmie\" in response to an experimental treatment. Instead, his research was a comparison of the unknown \"pygmie\" to humans and apes in order to determine the relationship between them.<\/li>\r\n \t<li>A second scenario in which comparative studies are common is when the physical scale or timeline of a question may prevent experimentation. For example, in the field of paleoclimatology, researchers have compared cores taken from sediments deposited millions of years ago in the world's oceans to see if the sedimentary composition is similar across all oceans or differs according to geographic location. Because the sediments in these cores were deposited millions of years ago, it would be impossible to obtain these results through the experimental method. Research designed to look at past events such as sediment cores deposited millions of years ago is referred to as retrospective research.<\/li>\r\n \t<li>A third common comparative scenario is when the ethical implications of an experimental treatment preclude an experimental design. Researchers who study the toxicity of environmental pollutants or the spread of disease in humans are precluded from purposefully exposing a group of individuals to the toxin or disease for ethical reasons. In these situations, researchers would set up a comparative study by identifying individuals who have been accidentally exposed to the pollutant or disease and comparing their symptoms to those of a control group of people who were not exposed. Research designed to look at events from the present into the future, such as a study looking at the development of symptoms in individuals exposed to a pollutant, is referred to as prospective research.<\/li>\r\n<\/ul>\r\n<\/blockquote>\r\nComparative science was significantly strengthened in the late 19th and early 20th century with the introduction of modern statistical <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/method\/pop\">methods<\/a>. These were used to <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/quantify\/pop\">quantify<\/a> the association between <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/variable\/pop\">variables<\/a> (see our <a href=\"https:\/\/www.visionlearning.com\/library\/module_viewer.php?mid=155\">Statistics in Science<\/a> module). Today, statistical methods are critical for quantifying the nature of relationships examined in many <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> studies. The <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/outcome\/pop\">outcome<\/a> of comparative <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a> is often presented in one of the following ways: as a <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/probability\/pop\">probability<\/a>, as a statement of <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/statistical+significance\/pop\">statistical significance<\/a>, or as a declaration of risk. For example, in 2007 Kristensen and Bjerkedal showed that there is a statistically significant relationship (at the 95% confidence level) between birth order and IQ by comparing test scores of first-born children to those of their younger siblings (Kristensen &amp; Bjerkedal, 2007). And numerous studies have contributed to the determination that the risk of developing lung cancer is 30 times greater in smokers than in nonsmokers (NCI, 1997).\r\n<div class=\"comprehension-checkpoint\">\r\n<p class=\"leader\">Comprehension Checkpoint<\/p>\r\n<p class=\"question\">Scientists may opt for comparative research where it would be unethical to conduct an experiment.<\/p>\r\n\r\n<form class=\"question\" name=\"cc5790\">\r\n<ul class=\"quiz-options\">\r\n \t<li class=\"option-a\"><label class=\"choice\" for=\"q1-5790-0-option-a\">true<\/label><\/li>\r\n \t<li class=\"option-b\"><label class=\"choice\" for=\"q1-5790-1-option-b\">false<\/label><\/li>\r\n<\/ul>\r\n<\/form><\/div>\r\n<\/section><section id=\"toc_3\" class=\"article-section\">\r\n<h2>Comparison in practice: The case of cigarettes<\/h2>\r\nIn 1919, Dr. George Dock, chairman of the Department of Medicine at Barnes Hospital in St. Louis, asked all of the third- and fourth-year medical students at the teaching hospital to observe an autopsy of a man with a disease so rare, he claimed, that most of the students would likely never see another case of it in their careers. With the medical students gathered around, the physicians conducting the autopsy observed that the patient's lungs were speckled with large dark masses of <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/cell\/pop\">cells<\/a> that had caused extensive damage to the lung tissue and had forced the airways to close and collapse. Dr. Alton Ochsner, one of the students who observed the autopsy, would write years later that \"I did not see another case until 1936, seventeen years later, when in a period of six months, I saw nine patients with cancer of the lung. \u2013 All the afflicted patients were men who smoked heavily and had smoked since World War I\" (Meyer, 1992).\r\n<figure><a title=\"BicycleWoman\" href=\"https:\/\/www.visionlearning.com\/img\/library\/large_images\/image_3895.jpg\"> <img src=\"https:\/\/www.visionlearning.com\/img\/library\/modules\/mid152\/Image\/VLObject-3895-080924010935.jpg\" alt=\"BicycleWoman\" \/> <\/a><figcaption>Figure 4: Image from a stereoptic card showing a woman smoking a cigarette circa 1900<\/figcaption><\/figure>\r\nThe American physician Dr. <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/Isaac+Newton\/pop\">Isaac<\/a> Adler was, in fact, the first scientist to propose a link between cigarette smoking and lung cancer in 1912, based on his <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/observation\/pop\">observation<\/a> that lung cancer patients often reported that they were smokers. Adler's observations, however, were anecdotal, and provided no scientific <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/evidence\/pop\">evidence<\/a> toward demonstrating a relationship. The German epidemiologist Franz M\u00fcller is credited with the first case-control study of smoking and lung cancer in the 1930s. M\u00fcller sent a survey to the relatives of individuals who had died of cancer, and asked them about the smoking habits of the deceased. Based on the responses he received, M\u00fcller reported a higher incidence of lung cancer among heavy smokers compared to light smokers. However, the study had a number of problems. First, it relied on the memory of relatives of deceased individuals rather than first-hand observations, and second, no statistical association was made. Soon after this, the tobacco industry began to sponsor <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a> with the biased goal of repudiating negative health claims against cigarettes (see our <a href=\"https:\/\/www.visionlearning.com\/library\/module_viewer.php?mid=162\">Scientific Institutions and Societies<\/a> module for more information on sponsored research).\r\n\r\nBeginning in the 1950s, several well-controlled <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> studies were initiated. In 1950, Ernest Wynder and Evarts Graham published a <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/retrospective\/pop\">retrospective<\/a> study comparing the smoking habits of 605 hospital patients with lung cancer to 780 hospital patients with other diseases (Wynder &amp; Graham, 1950). Their study showed that 1.3% of lung cancer patients were nonsmokers while 14.6% of patients with other diseases were nonsmokers. In addition, 51.2% of lung cancer patients were \"excessive\" smokers while only 19.1% of other patients were excessive smokers. Both of these comparisons proved to be statistically significant differences. The statisticians who analyzed the <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/data\/pop\">data<\/a> concluded:\r\n<blockquote>when the nonsmokers and the total of the high smoking classes of patients with lung cancer are compared with patients who have other diseases, we can reject the null hypothesis that smoking has no effect on the induction of cancer of the lungs.<\/blockquote>\r\nWynder and Graham also suggested that there might be a lag of ten years or more between the period of smoking in an individual and the onset of clinical symptoms of cancer. This would present a major challenge to researchers since any study that investigated the relationship between smoking and lung cancer in a <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/prospective\/pop\">prospective<\/a> fashion would have to last many years.\r\n\r\nRichard Doll and Austin Hill published a similar <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> study in 1950 in which they showed that there was a statistically higher incidence of smoking among lung cancer patients compared to patients with other diseases (Doll &amp; Hill, 1950). In their discussion, Doll and Hill raise an interesting point regarding comparative <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a> <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/method\/pop\">methods<\/a> by saying,\r\n<blockquote>This is not necessarily to state that smoking causes carcinoma of the lung. The association would occur if carcinoma of the lung caused people to smoke or if both attributes were end-effects of a common cause.<\/blockquote>\r\nThey go on to assert that because the habit of smoking was seen to develop before the onset of lung cancer, the argument that lung cancer leads to smoking can be rejected. They therefore conclude, \"that smoking is a factor, and an important factor, in the production of carcinoma of the lung.\"\r\n\r\nDespite this substantial <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/evidence\/pop\">evidence<\/a>, both the tobacco industry and unbiased scientists raised objections, claiming that the <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/retrospective\/pop\">retrospective<\/a> <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a> on smoking was \"limited, inconclusive, and controversial.\" The industry stated that the studies published did not demonstrate cause and effect, but rather a spurious association between two <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/variable\/pop\">variables<\/a>. Dr. Wilhelm Hueper of the National Cancer Institute, a scientist with a long history of research into occupational causes of cancers, argued that the emphasis on cigarettes as the only cause of lung cancer would compromise research support for other causes of lung cancer. <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/Fisher%2C+Ronald\/pop\">Ronald Fisher<\/a>, a renowned statistician, also was opposed to the conclusions of Doll and others, purportedly because they promoted a \"puritanical\" view of smoking.\r\n\r\nThe tobacco industry mounted an extensive campaign of misinformation, sponsoring and then citing <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a> that showed that smoking did not cause \"cardiac pain\" as a distraction from the studies that were being published regarding cigarettes and lung cancer. The industry also highlighted studies that showed that individuals who quit smoking suffered from mild depression, and they pointed to the fact that even some doctors themselves smoked cigarettes as <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/evidence\/pop\">evidence<\/a> that cigarettes were not harmful (Figure 5).\r\n<figure class=\"centered\"><a title=\"cigarette advertisement\" href=\"https:\/\/www.visionlearning.com\/img\/library\/large_images\/image_3896.jpg\"> <img src=\"https:\/\/www.visionlearning.com\/img\/library\/modules\/mid152\/Image\/VLObject-3896-080924010947.jpg\" alt=\"cigarette advertisement\" \/> <\/a><figcaption>Figure 5: Cigarette advertisement circa 1946.<\/figcaption><\/figure>\r\nWhile the scientific <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a> began to impact health officials and some legislators, the industry's ad campaign was effective. The US Federal Trade Commission banned tobacco companies from making health claims about their products in 1955. However, more significant regulation was averted. An editorial that appeared in the <em>New York Times<\/em> in 1963 summed up the national sentiment when it stated that the tobacco industry made a \"valid point,\" and the public should refrain from making a decision regarding cigarettes until further reports were issued by the US Surgeon General.\r\n\r\nIn 1951, Doll and Hill enrolled 40,000 British physicians in a <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/prospective\/pop\">prospective<\/a> <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> study to examine the association between smoking and the development of lung cancer. In contrast to the <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/retrospective\/pop\">retrospective<\/a> studies that followed patients with lung cancer back in time, the prospective study was designed to follow the group forward in time. In 1952, Drs. E. Cuyler Hammond and Daniel Horn enrolled 187,783 white males in the United States in a similar prospective study. And in 1959, the American Cancer Society (ACS) began the first of two large-scale prospective studies of the association between smoking and the development of lung cancer. The first ACS study, named Cancer Prevention Study I, enrolled more than 1 million individuals and tracked their health, smoking and other lifestyle habits, development of diseases, cause of death, and life expectancy for almost 13 years (Garfinkel, 1985).\r\n\r\nAll of the studies demonstrated that smokers are at a higher risk of developing and dying from lung cancer than nonsmokers. The ACS study further showed that smokers have elevated rates of other pulmonary diseases, coronary <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/artery\/pop\">artery<\/a> disease, stroke, and cardiovascular problems. The two ACS Cancer Prevention Studies would eventually show that 52% of deaths among smokers enrolled in the studies were attributed to cigarettes.\r\n\r\nIn the second half of the 20<sup>th<\/sup> century, <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/evidence\/pop\">evidence<\/a> from other scientific <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a> <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/method\/pop\">methods<\/a> would contribute multiple lines of evidence to the conclusion that cigarette smoke is a major cause of lung cancer:\r\n<blockquote>\r\n<ul class=\"bulleted\">\r\n \t<li><strong>Descriptive studies<\/strong> of the pathology of lungs of deceased smokers would demonstrate that smoking causes significant physiological damage to the lungs.<\/li>\r\n \t<li><strong>Experiments <\/strong>that exposed mice, rats, and other laboratory animals to cigarette smoke showed that it caused cancer in these animals (see our <a href=\"https:\/\/www.visionlearning.com\/library\/module_viewer.php?mid=150#Mice\">Experimentation in Science<\/a> module for more information).<\/li>\r\n \t<li><strong>Physiological models<\/strong> would help demonstrate the mechanism by which cigarette smoke causes cancer.<\/li>\r\n<\/ul>\r\n<\/blockquote>\r\nAs <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/evidence\/pop\">evidence<\/a> linking cigarette smoke to lung cancer and other diseases accumulated, the public, the legal community, and regulators slowly responded. In 1957, the US Surgeon General first acknowledged an association between smoking and lung cancer when a report was issued stating, \"It is clear that there is an increasing and consistent body of evidence that excessive cigarette smoking is one of the causative factors in lung cancer.\" In 1965, over objections by the tobacco industry and the American Medical Association, which had just accepted a $10 million grant from the tobacco companies, the US Congress passed the Federal Cigarette Labeling and Advertising Act, which required that cigarette packs carry the warning: \"Caution: Cigarette Smoking May Be Hazardous to Your Health.\" In 1967, the US Surgeon General issued a second report stating that cigarette smoking is the principal cause of lung cancer in the United States. While the tobacco companies found legal means to protect themselves for decades following this, in 1996, Brown and Williamson Tobacco Company was ordered to pay $750,000 in a tobacco liability lawsuit; it became the first liability award paid to an individual by a tobacco company.\r\n<div class=\"comprehension-checkpoint\">\r\n<p class=\"leader\">Comprehension Checkpoint<\/p>\r\n<p class=\"question\">_________ research looks at past events, while _________ research looks at events from the present into the future.<\/p>\r\n\r\n<form class=\"question\" name=\"cc5798\">\r\n<ul class=\"quiz-options\">\r\n \t<li class=\"option-a\"><label class=\"choice\" for=\"q1-5798-0-option-a\">Prospective, retrospective<\/label><\/li>\r\n \t<li class=\"option-b\"><label class=\"choice\" for=\"q1-5798-1-option-b\">Retrospective, prospective<\/label><\/li>\r\n<\/ul>\r\n<\/form><\/div>\r\n<\/section><section id=\"toc_4\" class=\"article-section\">\r\n<h2>Comparison across disciplines<\/h2>\r\nComparative studies are used in a host of scientific disciplines, from anthropology to archaeology, <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> biology, <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/epidemiology\/pop\">epidemiology<\/a>, psychology, and even forensic science. <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/DNA\/pop\">DNA<\/a> fingerprinting, a technique used to incriminate or exonerate a suspect using biological <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/evidence\/pop\">evidence<\/a>, is based on comparative science. In DNA fingerprinting, segments of DNA are isolated from a suspect and from biological evidence such as blood, semen, or other tissue left at a crime scene. Up to 20 different segments of DNA are compared between that of the suspect and the DNA found at the crime scene. If all of the segments match, the investigator can calculate the statistical <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/probability\/pop\">probability<\/a> that the DNA came from the suspect as opposed to someone else. Thus DNA matches are described in terms of a \"1 in 1 million\" or \"1 in 1 billion\" chance of error.\r\n\r\nComparative <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/method\/pop\">methods<\/a> are also commonly used in studies involving humans due to the ethical limits of experimental <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/treatment\/pop\">treatment<\/a>. For example, in 2007, Petter Kristensen and Tor Bjerkedal published a study in which they compared the IQ of over 250,000 male Norwegians in the military (Kristensen &amp; Bjerkedal, 2007). The researchers found a significant relationship between birth order and IQ, where the <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/average\/pop\">average<\/a> IQ of first-born male children was approximately three points higher than the average IQ of the second-born male in the same family. The researchers further showed that this relationship was correlated with social rather than biological factors, as second-born males who grew up in families in which the first-born child died had average IQs similar to other first-born children. One might imagine a scenario in which this type of study could be carried out experimentally, for example, purposefully removing first-born male children from certain families, but the ethics of such an <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/experiment\/pop\">experiment<\/a> preclude it from ever being conducted.\r\n\r\n<\/section><section id=\"toc_5\" class=\"article-section\">\r\n<h2>Limitations of comparative methods<\/h2>\r\nOne of the primary limitations of <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/method\/pop\">methods<\/a> is the control of other <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/variable\/pop\">variables<\/a> that might influence a study. For example, as pointed out by Doll and Hill in 1950, the association between smoking and cancer deaths could have meant that: a) smoking caused lung cancer, b) lung cancer caused individuals to take up smoking, or c) a third unknown variable caused lung cancer AND caused individuals to smoke (Doll &amp; Hill, 1950). As a result, comparative researchers often go to great lengths to choose two different study groups that are similar in almost all respects except for the <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/treatment\/pop\">treatment<\/a> in question. In fact, many comparative studies in humans are carried out on identical twins for this exact reason. For example, in the field of tobacco <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a>, dozens of comparative twin studies have been used to examine everything from the health effects of cigarette smoke to the genetic basis of addiction.\r\n<div class=\"comprehension-checkpoint\">\r\n<p class=\"leader\">Comprehension Checkpoint<\/p>\r\n<p class=\"question\">What is the benefit of comparing identical twins in research studies?<\/p>\r\n\r\n<form class=\"question\" name=\"cc5799\">\r\n<ul class=\"quiz-options\">\r\n \t<li class=\"option-a\"><label class=\"choice\" for=\"q1-5799-0-option-a\">It is easier to do research if the subjects know each other well. <\/label><\/li>\r\n \t<li class=\"option-b\"><label class=\"choice\" for=\"q1-5799-1-option-b\">It helps control variables that might influence a study.<\/label><\/li>\r\n<\/ul>\r\n<\/form><\/div>\r\n<\/section><section id=\"toc_6\" class=\"article-section\">\r\n<h2>Comparison in modern practice<\/h2>\r\n<figure><a title=\"keeling curve small\" href=\"https:\/\/www.visionlearning.com\/img\/library\/large_images\/image_3897.jpg\"> <img src=\"https:\/\/www.visionlearning.com\/img\/library\/modules\/mid152\/Image\/VLObject-3897-080924010952.jpg\" alt=\"keeling curve small\" \/> <\/a><figcaption>Figure 6: The \"Keeling curve,\" a long-term record of atmospheric CO<sub>2<\/sub> concentration measured at the Mauna Loa Observatory (Keeling et al.). Although the annual oscillations represent natural, seasonal variations, the long-term increase means that concentrations are higher than they have been in 400,000 years. Graphic courtesy of NASA's Earth Observatory.<\/figcaption><\/figure>\r\nDespite the lessons learned during the <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/debate\/pop\">debate<\/a> that ensued over the possible effects of cigarette smoke, misconceptions still surround <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> science. For example, in the late 1950s, <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/Keeling%2C+Charles\/pop\">Charles Keeling<\/a>, an oceanographer at the Scripps Institute of Oceanography, began to publish <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/data\/pop\">data<\/a> he had gathered from a long-term descriptive study of atmospheric carbon dioxide (CO<sub>2<\/sub>) levels at the Mauna Loa observatory in Hawaii (Keeling, 1958). Keeling observed that atmospheric CO<sub>2<\/sub> levels were increasing at a rapid rate (Figure 6). He and other researchers began to suspect that rising CO<sub>2<\/sub> levels were associated with increasing global <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/mean\/pop\">mean<\/a> temperatures, and several comparative studies have since correlated rising CO<sub>2<\/sub> levels with rising global temperature (Keeling, 1970). Together with <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a> from modeling studies (see our <a href=\"https:\/\/www.visionlearning.com\/library\/module_viewer.php?mid=153\">Modeling in Scientific Research<\/a> module), this research has provided <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/evidence\/pop\">evidence<\/a> for an association between global <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/climate\/pop\">climate<\/a> change and the burning of <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/fossil\/pop\">fossil<\/a> fuels (which <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/emit\/pop\">emits<\/a> CO<sub>2<\/sub>).\r\n\r\nYet in a move reminiscent of the fight launched by the tobacco companies, the oil and <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/fossil\/pop\">fossil<\/a> fuel industry launched a major public relations campaign against <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/climate\/pop\">climate<\/a> change <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a>. As late as 1989, scientists funded by the oil industry were producing reports that called the research on climate change \"noisy junk science\" (Roberts, 1989). As with the tobacco issue, challenges to early <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> studies tried to paint the <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/method\/pop\">method<\/a> as less reliable than experimental methods. But the challenges actually strengthened the science by prompting more researchers to launch investigations, thus providing multiple lines of <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/evidence\/pop\">evidence<\/a> supporting an association between atmospheric CO<sub>2<\/sub> <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/concentration\/pop\">concentrations<\/a> and climate change. As a result, the culmination of multiple lines of scientific evidence prompted the <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/Intergovernmental+Panel+on+Climate+Change\/pop\">Intergovernmental Panel on Climate Change<\/a> organized by the United Nations to issue a report stating that \"Warming of the climate <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/system\/pop\">system<\/a> is unequivocal,\" and \"Carbon dioxide is the most important anthropogenic greenhouse <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/gas\/pop\">gas<\/a> (IPCC, 2007).\"\r\n\r\nComparative studies are a critical part of the spectrum of <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a> <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/method\/pop\">methods<\/a> currently used in science. They allow scientists to apply a treatment-control design in settings that preclude experimentation, and they can provide invaluable information about the relationships between <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/variable\/pop\">variables<\/a>. The intense scrutiny that comparison has undergone in the public arena due to cases involving cigarettes and <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/climate\/pop\">climate<\/a> change has actually strengthened the method by clarifying its role in science and emphasizing the reliability of <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/data\/pop\">data<\/a> obtained from these studies.\r\n\r\n<\/section><section id=\"toc-999\" class=\"article-section\">\r\n<h3>Summary<\/h3>\r\nComparing and contrasting is a critical research tool for making sense of the world. Through scenarios in which scientists would likely choose to do comparative research, this module explores the differences and similarities between comparison and experimentation. Studies of the link between cigarette smoking and health illustrate how comparison along with other research methods provided solid evidence that cigarette smoke is a major cause of lung cancer.\r\n<h3>Key Concepts<\/h3>\r\n<ul class=\"bulleted\">\r\n \t<li>Comparison is used to determine and quantify relationships between two or more variables by observing different groups that either by choice or circumstance are exposed to different treatments.<\/li>\r\n \t<li>Comparison includes both retrospective studies that look at events that have already occurred, and prospective studies, that examine variables from the present forward.<\/li>\r\n \t<li>Comparative research is similar to experimentation in that it involves comparing a treatment group to a control, but it differs in that the treatment is observed rather than being consciously imposed due to ethical concerns, or because it is not possible, such as in a retrospective study.<\/li>\r\n<\/ul>\r\n<\/section><footer>\r\n<ul class=\"indented links\">\r\n \t<li>\r\n<h5>Further Reading<\/h5>\r\n<\/li>\r\n \t<li><a href=\"https:\/\/www.visionlearning.com\/en\/library\/Process-of-Science\/49\/Modeling-in-Scientific-Research\/153\">Modeling in Scientific Research<\/a><\/li>\r\n \t<li><a href=\"https:\/\/www.visionlearning.com\/en\/library\/Process-of-Science\/49\/Experimentation-in-Scientific-Research\/150\">Experimentation in Scientific Research<\/a><\/li>\r\n \t<li><a href=\"https:\/\/www.visionlearning.com\/en\/library\/Process-of-Science\/49\/Description-in-Scientific-Research\/151\">Description in Scientific Research<\/a><\/li>\r\n<\/ul>\r\n<a name=\"refs\"><\/a>\r\n<ul class=\"indented list\">\r\n \t<li>\r\n<h5>References<\/h5>\r\n<\/li>\r\n \t<li>Borio, G. (2003). <em>The twentieth century 1950\u20131999: The battle is joined<\/em>. Retrieved August 1, 2007, from the Tobacco Timeline, tobacco.org.<\/li>\r\n \t<li>Dremann, S. (2007). <a href=\"http:\/\/www.paloaltoonline.com\/weekly\/story.php?story_id=4648.\">Got a light?<\/a> <em>Palo Alto Weekly,<\/em> March 14, 2007.<\/li>\r\n \t<li>Doll, R. (1999). Tobacco: A medical history. <em>Journal of Urban Health: Bulletin of the New York Academy of Medicine 76<\/em>(3), 289-313.<\/li>\r\n \t<li>Doll, R., &amp; Hill, A. B. (1950). Smoking and carcinoma of the lung.<em> British Medical Journal, 2<\/em>(4682), 739-748.<\/li>\r\n \t<li>Enersen, O.D. (2007). <a href=\"http:\/\/www.whonamedit.com\/doctor.cfm\/3206.html\"><em>Edward Tyson<\/em><\/a>. Retrieved August 1, 2007, from Who Named It.<\/li>\r\n \t<li>Forest, C., Webster, M., &amp; Reilly, J. (2004). Narrowing uncertainty in global climate change. <em>The Industrial Physicist, AugSept,<\/em> 7\/23\/04, 22-25.<\/li>\r\n \t<li>Garfinkel, L. (1985). <em>Selection, follow-up, and analysis in the American Cancer Society prospective studies<\/em>. NCI Monograph No. 67, 49-52. Bethesda, MD: US Department of Health and Human Services, National Cancer Institute.<\/li>\r\n \t<li>IPCC \u2013 Intergovernmental Panel on Climate Change (2007). <em>Working Group I report: The physical science basis<\/em>. United Nations Environment Program, Geneva, http:\/\/www.ipcc.ch\/<\/li>\r\n \t<li>Keeling, C. (1958) The concentration and isotopic abundances of atmospheric carbon dioxide in rural areas. <em>Geochimica et Cosmochimica Acta, 13<\/em>(4), 322-334<\/li>\r\n \t<li>Keeling, C. (1970). Is carbon dioxide from fossil fuel changing man's environment? <em>Proceedings of the American Philosophical Society 114<\/em>(1), 10-17.<\/li>\r\n \t<li>Kristensen, P., &amp; Bjerkedal, T. (2007). Explaining the relation between birth order and intelligence. <em>Science, 316<\/em>(5832), 1717.<\/li>\r\n \t<li>Kundi, M. (2006). Causality and the interpretation of epidemiologic evidence. <em>Environmental Health Perspectives, 114<\/em>(7), 969-974.<\/li>\r\n \t<li>Maisel, A. Q. (1950). <a href=\"http:\/\/historymatters.gmu.edu\/d\/6447\/\">Don't smoke - unless you like it.<\/a> <em>Collier's,<\/em> November 4, 1950, 18.<\/li>\r\n \t<li>Meyer, J. A. (1992). Cigarette century. <em>American Heritage Magazine, 43<\/em>(8).<\/li>\r\n \t<li>NCI - National Cancer Institute (1997). Changes in cigarette-related disease risks and their implications for prevention and control. <em>Smoking and Tobacco Control Monographs,<\/em> National Institutes of Health.<\/li>\r\n \t<li>Newman, C. (1975). Edward Tyson. <em>British Medical Journal 4,<\/em> 96-97.<\/li>\r\n \t<li>Roberts, L. (1989). Global warming: Blaming the sun. <em>Science 246<\/em>(4933), 992-93.<\/li>\r\n \t<li>Russell, K. F. (1967). Edward Tyson's <em>Orang-Outang. Medical History, 11<\/em>(4), 417-423.<\/li>\r\n \t<li>Salsburg, D. (2001). <em>The lady tasting tea: How statistics revolutionized science in the twentieth century.<\/em> New York: W. H. Freeman &amp; Company.<\/li>\r\n \t<li>Witschi, H. (2001). A short history of lung cancer. <em>Toxicological Sciences 4<\/em>(1), 4-6.<\/li>\r\n \t<li>Wynder, E. L., &amp; Graham, E. A. (1950). <a href=\"http:\/\/tobacco.neu.edu\/box\/BOEKENBox\/medicalarticles.html\">Tobacco smoking as a possible etiological factor in bronchiogenic carcinoma<\/a>. <em>Journal of the American Medical Association 143<\/em>(4), 329-338.<\/li>\r\n<\/ul>\r\n<\/footer>","rendered":"<div class=\"article-introduction\">\n<p>nyone who has stared at a chimpanzee in a zoo (Figure 1) has probably wondered about the animal&#8217;s similarity to humans. Chimps make facial expressions that resemble humans, use their hands in much the same way we do, are adept at using different objects as tools, and even laugh when they are tickled. It may not be surprising to learn then that when the first captured chimpanzees were brought to Europe in the 17<sup>th<\/sup> century, people were confused, labeling the animals &#8220;pygmies&#8221; and speculating that they were stunted versions of &#8220;full-grown&#8221; humans. A London physician named <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/Tyson%2C+Edward\/pop\">Edward Tyson<\/a> obtained a &#8220;pygmie&#8221; that had died of an infection shortly after arriving in London, and began a systematic study of the animal that cataloged the differences between chimpanzees and humans, thus helping to establish <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a> as a scientific <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/method\/pop\">method<\/a>.<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/www.visionlearning.com\/img\/library\/modules\/mid152\/Image\/VLObject-1987-030729120734.jpg\" alt=\"chimpanzee\" \/><figcaption>Figure 1: A chimpanzee <span class=\"credit\">image \u00a9 Corel Corporation<\/span><\/figcaption><\/figure>\n<\/div>\n<section id=\"toc_1\" class=\"article-section\">\n<h2>A brief history of comparative methods<\/h2>\n<p>In 1698, Tyson, a member of the Royal Society of London, began a detailed dissection of the &#8220;pygmie&#8221; he had obtained and published his findings in the 1699 work: <em>Orang-Outang, sive Homo Sylvestris: or, the Anatomy of a Pygmie Compared with that of a Monkey, an Ape, and a Man<\/em>. The title of the work further reflects the misconception that existed at the time \u2013 Tyson did not use the term Orang-Outang in its modern sense to refer to the orangutan; he used it in its literal translation from the Malay language as &#8220;man of the woods,&#8221; as that is how the chimps were viewed.<\/p>\n<p>Tyson took great care in his dissection. He precisely measured and compared a number of anatomical <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/variable\/pop\">variables<\/a> such as brain size of the &#8220;pygmie,&#8221; ape, and human. He recorded his measurements of the &#8220;pygmie,&#8221; even down to the direction in which the animal&#8217;s hair grew: &#8220;The tendency of the Hair of all of the Body was downwards; but only from the Wrists to the Elbows &#8217;twas upwards&#8221; (Russell, 1967). Aided by William Cowper, Tyson made drawings of various anatomical structures, taking great care to accurately depict the dimensions of these structures so that they could be compared to those in humans (Figure 2). His systematic <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> study of the dimensions of anatomical structures in the chimp, ape, and human led him to state:<\/p>\n<blockquote><p>in the Organization of abundance of its Parts, it more approached to the Structure of the same in Men: But where it differs from a Man, there it resembles plainly the Common Ape, more than any other Animal. (Russell, 1967)<\/p><\/blockquote>\n<p>Tyson&#8217;s <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> studies proved exceptionally accurate and his <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a> was used by others, including <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/Huxley%2C+Thomas+Henry\/pop\">Thomas Henry Huxley<\/a> in <em>Evidence as to Man&#8217;s Place in Nature<\/em> (1863) and <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/Darwin%2C+Charles\/pop\">Charles Darwin<\/a> in <em>The Descent of Man<\/em> (1871).<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/www.visionlearning.com\/img\/library\/modules\/mid152\/Image\/VLObject-3893-080924010924.jpg\" alt=\"pygmie and skeleton\" \/><figcaption>Figure 2: Edward Tyson&#8217;s drawing of the external appearance of a &#8220;pygmie&#8221; (left) and the animal&#8217;s skeleton (right) from <em>The Anatomy of a Pygmie Compared with that of a Monkey, an Ape, and a Man<\/em> from the second edition, London, printed for T. Osborne, 1751.<\/figcaption><\/figure>\n<p>Tyson&#8217;s methodical and scientific approach to anatomical dissection contributed to the development of evolutionary <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/theory\/pop\">theory<\/a> and helped establish the field of <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> anatomy. Further, Tyson&#8217;s work helps to highlight the importance of comparison as a scientific <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a> <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/method\/pop\">method<\/a>.<\/p>\n<\/section>\n<section id=\"toc_2\" class=\"article-section\">\n<h2>Comparison as a scientific research method<\/h2>\n<p>Comparative <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a> represents one approach in the spectrum of scientific research <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/method\/pop\">methods<\/a> and in some ways is a hybrid of other methods, drawing on aspects of both experimental science (see our <a href=\"https:\/\/www.visionlearning.com\/library\/module_viewer.php?mid=150\">Experimentation in Science<\/a> module) and descriptive research (see our <a href=\"https:\/\/www.visionlearning.com\/library\/module_viewer.php?mid=151\">Description in Science<\/a> module). Similar to experimentation, comparison seeks to decipher the relationship between two or more <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/variable\/pop\">variables<\/a> by documenting observed differences and similarities between two or more <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/subject\/pop\">subjects<\/a> or groups. In contrast to experimentation, the <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> researcher does not subject one of those groups to a <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/treatment\/pop\">treatment<\/a>, but rather observes a group that either by choice or circumstance has been subject to a treatment. Thus comparison involves <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/observation\/pop\">observation<\/a> in a more &#8220;natural&#8221; setting, not subject to experimental confines, and in this way evokes similarities with description.<\/p>\n<p>Importantly, the simple comparison of two <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/variable\/pop\">variables<\/a> or objects is not <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a>. Tyson&#8217;s work would not have been considered scientific research if he had simply noted that &#8220;pygmies&#8221; looked like humans without measuring bone lengths and hair growth patterns. Instead, comparative research involves the systematic cataloging of the nature and\/or behavior of two or more variables, and the quantification of the relationship between them.<\/p>\n<figure><a title=\"skeleton chimpanzee\" href=\"https:\/\/www.visionlearning.com\/img\/library\/large_images\/image_3894.jpg\"> <img decoding=\"async\" src=\"https:\/\/www.visionlearning.com\/img\/library\/modules\/mid152\/Image\/VLObject-3894-080924010927.jpg\" alt=\"skeleton chimpanzee\" \/> <\/a><figcaption>Figure 3: Skeleton of the juvenile chimpanzee dissected by Edward Tyson, currently displayed at the Natural History Museum, London. <span class=\"credit\">image \u00a9 Peter Kaminski<\/span><\/figcaption><\/figure>\n<p>While the choice of which <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a> <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/method\/pop\">method<\/a> to use is a personal decision based in part on the training of the researchers conducting the study, there are a number of scenarios in which <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> research would likely be the primary choice.<\/p>\n<blockquote>\n<ul class=\"bulleted\">\n<li>The first scenario is one in which the scientist is not trying to measure a response to change, but rather he or she may be trying to understand the similarities and differences between two subjects. For example, Tyson was not observing a change in his &#8220;pygmie&#8221; in response to an experimental treatment. Instead, his research was a comparison of the unknown &#8220;pygmie&#8221; to humans and apes in order to determine the relationship between them.<\/li>\n<li>A second scenario in which comparative studies are common is when the physical scale or timeline of a question may prevent experimentation. For example, in the field of paleoclimatology, researchers have compared cores taken from sediments deposited millions of years ago in the world&#8217;s oceans to see if the sedimentary composition is similar across all oceans or differs according to geographic location. Because the sediments in these cores were deposited millions of years ago, it would be impossible to obtain these results through the experimental method. Research designed to look at past events such as sediment cores deposited millions of years ago is referred to as retrospective research.<\/li>\n<li>A third common comparative scenario is when the ethical implications of an experimental treatment preclude an experimental design. Researchers who study the toxicity of environmental pollutants or the spread of disease in humans are precluded from purposefully exposing a group of individuals to the toxin or disease for ethical reasons. In these situations, researchers would set up a comparative study by identifying individuals who have been accidentally exposed to the pollutant or disease and comparing their symptoms to those of a control group of people who were not exposed. Research designed to look at events from the present into the future, such as a study looking at the development of symptoms in individuals exposed to a pollutant, is referred to as prospective research.<\/li>\n<\/ul>\n<\/blockquote>\n<p>Comparative science was significantly strengthened in the late 19th and early 20th century with the introduction of modern statistical <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/method\/pop\">methods<\/a>. These were used to <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/quantify\/pop\">quantify<\/a> the association between <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/variable\/pop\">variables<\/a> (see our <a href=\"https:\/\/www.visionlearning.com\/library\/module_viewer.php?mid=155\">Statistics in Science<\/a> module). Today, statistical methods are critical for quantifying the nature of relationships examined in many <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> studies. The <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/outcome\/pop\">outcome<\/a> of comparative <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a> is often presented in one of the following ways: as a <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/probability\/pop\">probability<\/a>, as a statement of <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/statistical+significance\/pop\">statistical significance<\/a>, or as a declaration of risk. For example, in 2007 Kristensen and Bjerkedal showed that there is a statistically significant relationship (at the 95% confidence level) between birth order and IQ by comparing test scores of first-born children to those of their younger siblings (Kristensen &amp; Bjerkedal, 2007). And numerous studies have contributed to the determination that the risk of developing lung cancer is 30 times greater in smokers than in nonsmokers (NCI, 1997).<\/p>\n<div class=\"comprehension-checkpoint\">\n<p class=\"leader\">Comprehension Checkpoint<\/p>\n<p class=\"question\">Scientists may opt for comparative research where it would be unethical to conduct an experiment.<\/p>\n<form class=\"question\" action=\"action\" id=\"cc5790\">\n<ul class=\"quiz-options\">\n<li class=\"option-a\"><label class=\"choice\" for=\"q1-5790-0-option-a\">true<\/label><\/li>\n<li class=\"option-b\"><label class=\"choice\" for=\"q1-5790-1-option-b\">false<\/label><\/li>\n<\/ul>\n<\/form>\n<\/div>\n<\/section>\n<section id=\"toc_3\" class=\"article-section\">\n<h2>Comparison in practice: The case of cigarettes<\/h2>\n<p>In 1919, Dr. George Dock, chairman of the Department of Medicine at Barnes Hospital in St. Louis, asked all of the third- and fourth-year medical students at the teaching hospital to observe an autopsy of a man with a disease so rare, he claimed, that most of the students would likely never see another case of it in their careers. With the medical students gathered around, the physicians conducting the autopsy observed that the patient&#8217;s lungs were speckled with large dark masses of <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/cell\/pop\">cells<\/a> that had caused extensive damage to the lung tissue and had forced the airways to close and collapse. Dr. Alton Ochsner, one of the students who observed the autopsy, would write years later that &#8220;I did not see another case until 1936, seventeen years later, when in a period of six months, I saw nine patients with cancer of the lung. \u2013 All the afflicted patients were men who smoked heavily and had smoked since World War I&#8221; (Meyer, 1992).<\/p>\n<figure><a title=\"BicycleWoman\" href=\"https:\/\/www.visionlearning.com\/img\/library\/large_images\/image_3895.jpg\"> <img decoding=\"async\" src=\"https:\/\/www.visionlearning.com\/img\/library\/modules\/mid152\/Image\/VLObject-3895-080924010935.jpg\" alt=\"BicycleWoman\" \/> <\/a><figcaption>Figure 4: Image from a stereoptic card showing a woman smoking a cigarette circa 1900<\/figcaption><\/figure>\n<p>The American physician Dr. <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/Isaac+Newton\/pop\">Isaac<\/a> Adler was, in fact, the first scientist to propose a link between cigarette smoking and lung cancer in 1912, based on his <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/observation\/pop\">observation<\/a> that lung cancer patients often reported that they were smokers. Adler&#8217;s observations, however, were anecdotal, and provided no scientific <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/evidence\/pop\">evidence<\/a> toward demonstrating a relationship. The German epidemiologist Franz M\u00fcller is credited with the first case-control study of smoking and lung cancer in the 1930s. M\u00fcller sent a survey to the relatives of individuals who had died of cancer, and asked them about the smoking habits of the deceased. Based on the responses he received, M\u00fcller reported a higher incidence of lung cancer among heavy smokers compared to light smokers. However, the study had a number of problems. First, it relied on the memory of relatives of deceased individuals rather than first-hand observations, and second, no statistical association was made. Soon after this, the tobacco industry began to sponsor <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a> with the biased goal of repudiating negative health claims against cigarettes (see our <a href=\"https:\/\/www.visionlearning.com\/library\/module_viewer.php?mid=162\">Scientific Institutions and Societies<\/a> module for more information on sponsored research).<\/p>\n<p>Beginning in the 1950s, several well-controlled <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> studies were initiated. In 1950, Ernest Wynder and Evarts Graham published a <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/retrospective\/pop\">retrospective<\/a> study comparing the smoking habits of 605 hospital patients with lung cancer to 780 hospital patients with other diseases (Wynder &amp; Graham, 1950). Their study showed that 1.3% of lung cancer patients were nonsmokers while 14.6% of patients with other diseases were nonsmokers. In addition, 51.2% of lung cancer patients were &#8220;excessive&#8221; smokers while only 19.1% of other patients were excessive smokers. Both of these comparisons proved to be statistically significant differences. The statisticians who analyzed the <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/data\/pop\">data<\/a> concluded:<\/p>\n<blockquote><p>when the nonsmokers and the total of the high smoking classes of patients with lung cancer are compared with patients who have other diseases, we can reject the null hypothesis that smoking has no effect on the induction of cancer of the lungs.<\/p><\/blockquote>\n<p>Wynder and Graham also suggested that there might be a lag of ten years or more between the period of smoking in an individual and the onset of clinical symptoms of cancer. This would present a major challenge to researchers since any study that investigated the relationship between smoking and lung cancer in a <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/prospective\/pop\">prospective<\/a> fashion would have to last many years.<\/p>\n<p>Richard Doll and Austin Hill published a similar <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> study in 1950 in which they showed that there was a statistically higher incidence of smoking among lung cancer patients compared to patients with other diseases (Doll &amp; Hill, 1950). In their discussion, Doll and Hill raise an interesting point regarding comparative <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a> <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/method\/pop\">methods<\/a> by saying,<\/p>\n<blockquote><p>This is not necessarily to state that smoking causes carcinoma of the lung. The association would occur if carcinoma of the lung caused people to smoke or if both attributes were end-effects of a common cause.<\/p><\/blockquote>\n<p>They go on to assert that because the habit of smoking was seen to develop before the onset of lung cancer, the argument that lung cancer leads to smoking can be rejected. They therefore conclude, &#8220;that smoking is a factor, and an important factor, in the production of carcinoma of the lung.&#8221;<\/p>\n<p>Despite this substantial <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/evidence\/pop\">evidence<\/a>, both the tobacco industry and unbiased scientists raised objections, claiming that the <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/retrospective\/pop\">retrospective<\/a> <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a> on smoking was &#8220;limited, inconclusive, and controversial.&#8221; The industry stated that the studies published did not demonstrate cause and effect, but rather a spurious association between two <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/variable\/pop\">variables<\/a>. Dr. Wilhelm Hueper of the National Cancer Institute, a scientist with a long history of research into occupational causes of cancers, argued that the emphasis on cigarettes as the only cause of lung cancer would compromise research support for other causes of lung cancer. <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/Fisher%2C+Ronald\/pop\">Ronald Fisher<\/a>, a renowned statistician, also was opposed to the conclusions of Doll and others, purportedly because they promoted a &#8220;puritanical&#8221; view of smoking.<\/p>\n<p>The tobacco industry mounted an extensive campaign of misinformation, sponsoring and then citing <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a> that showed that smoking did not cause &#8220;cardiac pain&#8221; as a distraction from the studies that were being published regarding cigarettes and lung cancer. The industry also highlighted studies that showed that individuals who quit smoking suffered from mild depression, and they pointed to the fact that even some doctors themselves smoked cigarettes as <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/evidence\/pop\">evidence<\/a> that cigarettes were not harmful (Figure 5).<\/p>\n<figure class=\"centered\"><a title=\"cigarette advertisement\" href=\"https:\/\/www.visionlearning.com\/img\/library\/large_images\/image_3896.jpg\"> <img decoding=\"async\" src=\"https:\/\/www.visionlearning.com\/img\/library\/modules\/mid152\/Image\/VLObject-3896-080924010947.jpg\" alt=\"cigarette advertisement\" \/> <\/a><figcaption>Figure 5: Cigarette advertisement circa 1946.<\/figcaption><\/figure>\n<p>While the scientific <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a> began to impact health officials and some legislators, the industry&#8217;s ad campaign was effective. The US Federal Trade Commission banned tobacco companies from making health claims about their products in 1955. However, more significant regulation was averted. An editorial that appeared in the <em>New York Times<\/em> in 1963 summed up the national sentiment when it stated that the tobacco industry made a &#8220;valid point,&#8221; and the public should refrain from making a decision regarding cigarettes until further reports were issued by the US Surgeon General.<\/p>\n<p>In 1951, Doll and Hill enrolled 40,000 British physicians in a <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/prospective\/pop\">prospective<\/a> <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> study to examine the association between smoking and the development of lung cancer. In contrast to the <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/retrospective\/pop\">retrospective<\/a> studies that followed patients with lung cancer back in time, the prospective study was designed to follow the group forward in time. In 1952, Drs. E. Cuyler Hammond and Daniel Horn enrolled 187,783 white males in the United States in a similar prospective study. And in 1959, the American Cancer Society (ACS) began the first of two large-scale prospective studies of the association between smoking and the development of lung cancer. The first ACS study, named Cancer Prevention Study I, enrolled more than 1 million individuals and tracked their health, smoking and other lifestyle habits, development of diseases, cause of death, and life expectancy for almost 13 years (Garfinkel, 1985).<\/p>\n<p>All of the studies demonstrated that smokers are at a higher risk of developing and dying from lung cancer than nonsmokers. The ACS study further showed that smokers have elevated rates of other pulmonary diseases, coronary <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/artery\/pop\">artery<\/a> disease, stroke, and cardiovascular problems. The two ACS Cancer Prevention Studies would eventually show that 52% of deaths among smokers enrolled in the studies were attributed to cigarettes.<\/p>\n<p>In the second half of the 20<sup>th<\/sup> century, <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/evidence\/pop\">evidence<\/a> from other scientific <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a> <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/method\/pop\">methods<\/a> would contribute multiple lines of evidence to the conclusion that cigarette smoke is a major cause of lung cancer:<\/p>\n<blockquote>\n<ul class=\"bulleted\">\n<li><strong>Descriptive studies<\/strong> of the pathology of lungs of deceased smokers would demonstrate that smoking causes significant physiological damage to the lungs.<\/li>\n<li><strong>Experiments <\/strong>that exposed mice, rats, and other laboratory animals to cigarette smoke showed that it caused cancer in these animals (see our <a href=\"https:\/\/www.visionlearning.com\/library\/module_viewer.php?mid=150#Mice\">Experimentation in Science<\/a> module for more information).<\/li>\n<li><strong>Physiological models<\/strong> would help demonstrate the mechanism by which cigarette smoke causes cancer.<\/li>\n<\/ul>\n<\/blockquote>\n<p>As <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/evidence\/pop\">evidence<\/a> linking cigarette smoke to lung cancer and other diseases accumulated, the public, the legal community, and regulators slowly responded. In 1957, the US Surgeon General first acknowledged an association between smoking and lung cancer when a report was issued stating, &#8220;It is clear that there is an increasing and consistent body of evidence that excessive cigarette smoking is one of the causative factors in lung cancer.&#8221; In 1965, over objections by the tobacco industry and the American Medical Association, which had just accepted a $10 million grant from the tobacco companies, the US Congress passed the Federal Cigarette Labeling and Advertising Act, which required that cigarette packs carry the warning: &#8220;Caution: Cigarette Smoking May Be Hazardous to Your Health.&#8221; In 1967, the US Surgeon General issued a second report stating that cigarette smoking is the principal cause of lung cancer in the United States. While the tobacco companies found legal means to protect themselves for decades following this, in 1996, Brown and Williamson Tobacco Company was ordered to pay $750,000 in a tobacco liability lawsuit; it became the first liability award paid to an individual by a tobacco company.<\/p>\n<div class=\"comprehension-checkpoint\">\n<p class=\"leader\">Comprehension Checkpoint<\/p>\n<p class=\"question\">_________ research looks at past events, while _________ research looks at events from the present into the future.<\/p>\n<form class=\"question\" action=\"action\" id=\"cc5798\">\n<ul class=\"quiz-options\">\n<li class=\"option-a\"><label class=\"choice\" for=\"q1-5798-0-option-a\">Prospective, retrospective<\/label><\/li>\n<li class=\"option-b\"><label class=\"choice\" for=\"q1-5798-1-option-b\">Retrospective, prospective<\/label><\/li>\n<\/ul>\n<\/form>\n<\/div>\n<\/section>\n<section id=\"toc_4\" class=\"article-section\">\n<h2>Comparison across disciplines<\/h2>\n<p>Comparative studies are used in a host of scientific disciplines, from anthropology to archaeology, <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> biology, <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/epidemiology\/pop\">epidemiology<\/a>, psychology, and even forensic science. <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/DNA\/pop\">DNA<\/a> fingerprinting, a technique used to incriminate or exonerate a suspect using biological <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/evidence\/pop\">evidence<\/a>, is based on comparative science. In DNA fingerprinting, segments of DNA are isolated from a suspect and from biological evidence such as blood, semen, or other tissue left at a crime scene. Up to 20 different segments of DNA are compared between that of the suspect and the DNA found at the crime scene. If all of the segments match, the investigator can calculate the statistical <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/probability\/pop\">probability<\/a> that the DNA came from the suspect as opposed to someone else. Thus DNA matches are described in terms of a &#8220;1 in 1 million&#8221; or &#8220;1 in 1 billion&#8221; chance of error.<\/p>\n<p>Comparative <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/method\/pop\">methods<\/a> are also commonly used in studies involving humans due to the ethical limits of experimental <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/treatment\/pop\">treatment<\/a>. For example, in 2007, Petter Kristensen and Tor Bjerkedal published a study in which they compared the IQ of over 250,000 male Norwegians in the military (Kristensen &amp; Bjerkedal, 2007). The researchers found a significant relationship between birth order and IQ, where the <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/average\/pop\">average<\/a> IQ of first-born male children was approximately three points higher than the average IQ of the second-born male in the same family. The researchers further showed that this relationship was correlated with social rather than biological factors, as second-born males who grew up in families in which the first-born child died had average IQs similar to other first-born children. One might imagine a scenario in which this type of study could be carried out experimentally, for example, purposefully removing first-born male children from certain families, but the ethics of such an <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/experiment\/pop\">experiment<\/a> preclude it from ever being conducted.<\/p>\n<\/section>\n<section id=\"toc_5\" class=\"article-section\">\n<h2>Limitations of comparative methods<\/h2>\n<p>One of the primary limitations of <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/method\/pop\">methods<\/a> is the control of other <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/variable\/pop\">variables<\/a> that might influence a study. For example, as pointed out by Doll and Hill in 1950, the association between smoking and cancer deaths could have meant that: a) smoking caused lung cancer, b) lung cancer caused individuals to take up smoking, or c) a third unknown variable caused lung cancer AND caused individuals to smoke (Doll &amp; Hill, 1950). As a result, comparative researchers often go to great lengths to choose two different study groups that are similar in almost all respects except for the <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/treatment\/pop\">treatment<\/a> in question. In fact, many comparative studies in humans are carried out on identical twins for this exact reason. For example, in the field of tobacco <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a>, dozens of comparative twin studies have been used to examine everything from the health effects of cigarette smoke to the genetic basis of addiction.<\/p>\n<div class=\"comprehension-checkpoint\">\n<p class=\"leader\">Comprehension Checkpoint<\/p>\n<p class=\"question\">What is the benefit of comparing identical twins in research studies?<\/p>\n<form class=\"question\" action=\"action\" id=\"cc5799\">\n<ul class=\"quiz-options\">\n<li class=\"option-a\"><label class=\"choice\" for=\"q1-5799-0-option-a\">It is easier to do research if the subjects know each other well. <\/label><\/li>\n<li class=\"option-b\"><label class=\"choice\" for=\"q1-5799-1-option-b\">It helps control variables that might influence a study.<\/label><\/li>\n<\/ul>\n<\/form>\n<\/div>\n<\/section>\n<section id=\"toc_6\" class=\"article-section\">\n<h2>Comparison in modern practice<\/h2>\n<figure><a title=\"keeling curve small\" href=\"https:\/\/www.visionlearning.com\/img\/library\/large_images\/image_3897.jpg\"> <img decoding=\"async\" src=\"https:\/\/www.visionlearning.com\/img\/library\/modules\/mid152\/Image\/VLObject-3897-080924010952.jpg\" alt=\"keeling curve small\" \/> <\/a><figcaption>Figure 6: The &#8220;Keeling curve,&#8221; a long-term record of atmospheric CO<sub>2<\/sub> concentration measured at the Mauna Loa Observatory (Keeling et al.). Although the annual oscillations represent natural, seasonal variations, the long-term increase means that concentrations are higher than they have been in 400,000 years. Graphic courtesy of NASA&#8217;s Earth Observatory.<\/figcaption><\/figure>\n<p>Despite the lessons learned during the <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/debate\/pop\">debate<\/a> that ensued over the possible effects of cigarette smoke, misconceptions still surround <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> science. For example, in the late 1950s, <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/Keeling%2C+Charles\/pop\">Charles Keeling<\/a>, an oceanographer at the Scripps Institute of Oceanography, began to publish <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/data\/pop\">data<\/a> he had gathered from a long-term descriptive study of atmospheric carbon dioxide (CO<sub>2<\/sub>) levels at the Mauna Loa observatory in Hawaii (Keeling, 1958). Keeling observed that atmospheric CO<sub>2<\/sub> levels were increasing at a rapid rate (Figure 6). He and other researchers began to suspect that rising CO<sub>2<\/sub> levels were associated with increasing global <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/mean\/pop\">mean<\/a> temperatures, and several comparative studies have since correlated rising CO<sub>2<\/sub> levels with rising global temperature (Keeling, 1970). Together with <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a> from modeling studies (see our <a href=\"https:\/\/www.visionlearning.com\/library\/module_viewer.php?mid=153\">Modeling in Scientific Research<\/a> module), this research has provided <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/evidence\/pop\">evidence<\/a> for an association between global <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/climate\/pop\">climate<\/a> change and the burning of <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/fossil\/pop\">fossil<\/a> fuels (which <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/emit\/pop\">emits<\/a> CO<sub>2<\/sub>).<\/p>\n<p>Yet in a move reminiscent of the fight launched by the tobacco companies, the oil and <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/fossil\/pop\">fossil<\/a> fuel industry launched a major public relations campaign against <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/climate\/pop\">climate<\/a> change <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a>. As late as 1989, scientists funded by the oil industry were producing reports that called the research on climate change &#8220;noisy junk science&#8221; (Roberts, 1989). As with the tobacco issue, challenges to early <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/comparative\/pop\">comparative<\/a> studies tried to paint the <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/method\/pop\">method<\/a> as less reliable than experimental methods. But the challenges actually strengthened the science by prompting more researchers to launch investigations, thus providing multiple lines of <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/evidence\/pop\">evidence<\/a> supporting an association between atmospheric CO<sub>2<\/sub> <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/concentration\/pop\">concentrations<\/a> and climate change. As a result, the culmination of multiple lines of scientific evidence prompted the <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/Intergovernmental+Panel+on+Climate+Change\/pop\">Intergovernmental Panel on Climate Change<\/a> organized by the United Nations to issue a report stating that &#8220;Warming of the climate <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/system\/pop\">system<\/a> is unequivocal,&#8221; and &#8220;Carbon dioxide is the most important anthropogenic greenhouse <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/gas\/pop\">gas<\/a> (IPCC, 2007).&#8221;<\/p>\n<p>Comparative studies are a critical part of the spectrum of <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/research\/pop\">research<\/a> <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/method\/pop\">methods<\/a> currently used in science. They allow scientists to apply a treatment-control design in settings that preclude experimentation, and they can provide invaluable information about the relationships between <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/variable\/pop\">variables<\/a>. The intense scrutiny that comparison has undergone in the public arena due to cases involving cigarettes and <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/climate\/pop\">climate<\/a> change has actually strengthened the method by clarifying its role in science and emphasizing the reliability of <a class=\"term\" title=\"\" href=\"https:\/\/www.visionlearning.com\/en\/glossary\/view\/data\/pop\">data<\/a> obtained from these studies.<\/p>\n<\/section>\n<section id=\"toc-999\" class=\"article-section\">\n<h3>Summary<\/h3>\n<p>Comparing and contrasting is a critical research tool for making sense of the world. Through scenarios in which scientists would likely choose to do comparative research, this module explores the differences and similarities between comparison and experimentation. Studies of the link between cigarette smoking and health illustrate how comparison along with other research methods provided solid evidence that cigarette smoke is a major cause of lung cancer.<\/p>\n<h3>Key Concepts<\/h3>\n<ul class=\"bulleted\">\n<li>Comparison is used to determine and quantify relationships between two or more variables by observing different groups that either by choice or circumstance are exposed to different treatments.<\/li>\n<li>Comparison includes both retrospective studies that look at events that have already occurred, and prospective studies, that examine variables from the present forward.<\/li>\n<li>Comparative research is similar to experimentation in that it involves comparing a treatment group to a control, but it differs in that the treatment is observed rather than being consciously imposed due to ethical concerns, or because it is not possible, such as in a retrospective study.<\/li>\n<\/ul>\n<\/section>\n<footer>\n<ul class=\"indented links\">\n<li>\n<h5>Further Reading<\/h5>\n<\/li>\n<li><a href=\"https:\/\/www.visionlearning.com\/en\/library\/Process-of-Science\/49\/Modeling-in-Scientific-Research\/153\">Modeling in Scientific Research<\/a><\/li>\n<li><a href=\"https:\/\/www.visionlearning.com\/en\/library\/Process-of-Science\/49\/Experimentation-in-Scientific-Research\/150\">Experimentation in Scientific Research<\/a><\/li>\n<li><a href=\"https:\/\/www.visionlearning.com\/en\/library\/Process-of-Science\/49\/Description-in-Scientific-Research\/151\">Description in Scientific Research<\/a><\/li>\n<\/ul>\n<p><a name=\"refs\" id=\"refs\"><\/a><\/p>\n<ul class=\"indented list\">\n<li>\n<h5>References<\/h5>\n<\/li>\n<li>Borio, G. (2003). <em>The twentieth century 1950\u20131999: The battle is joined<\/em>. Retrieved August 1, 2007, from the Tobacco Timeline, tobacco.org.<\/li>\n<li>Dremann, S. (2007). <a href=\"http:\/\/www.paloaltoonline.com\/weekly\/story.php?story_id=4648.\">Got a light?<\/a> <em>Palo Alto Weekly,<\/em> March 14, 2007.<\/li>\n<li>Doll, R. (1999). Tobacco: A medical history. <em>Journal of Urban Health: Bulletin of the New York Academy of Medicine 76<\/em>(3), 289-313.<\/li>\n<li>Doll, R., &amp; Hill, A. B. (1950). Smoking and carcinoma of the lung.<em> British Medical Journal, 2<\/em>(4682), 739-748.<\/li>\n<li>Enersen, O.D. (2007). <a href=\"http:\/\/www.whonamedit.com\/doctor.cfm\/3206.html\"><em>Edward Tyson<\/em><\/a>. Retrieved August 1, 2007, from Who Named It.<\/li>\n<li>Forest, C., Webster, M., &amp; Reilly, J. (2004). Narrowing uncertainty in global climate change. <em>The Industrial Physicist, AugSept,<\/em> 7\/23\/04, 22-25.<\/li>\n<li>Garfinkel, L. (1985). <em>Selection, follow-up, and analysis in the American Cancer Society prospective studies<\/em>. NCI Monograph No. 67, 49-52. Bethesda, MD: US Department of Health and Human Services, National Cancer Institute.<\/li>\n<li>IPCC \u2013 Intergovernmental Panel on Climate Change (2007). <em>Working Group I report: The physical science basis<\/em>. United Nations Environment Program, Geneva, http:\/\/www.ipcc.ch\/<\/li>\n<li>Keeling, C. (1958) The concentration and isotopic abundances of atmospheric carbon dioxide in rural areas. <em>Geochimica et Cosmochimica Acta, 13<\/em>(4), 322-334<\/li>\n<li>Keeling, C. (1970). Is carbon dioxide from fossil fuel changing man&#8217;s environment? <em>Proceedings of the American Philosophical Society 114<\/em>(1), 10-17.<\/li>\n<li>Kristensen, P., &amp; Bjerkedal, T. (2007). Explaining the relation between birth order and intelligence. <em>Science, 316<\/em>(5832), 1717.<\/li>\n<li>Kundi, M. (2006). Causality and the interpretation of epidemiologic evidence. <em>Environmental Health Perspectives, 114<\/em>(7), 969-974.<\/li>\n<li>Maisel, A. Q. (1950). <a href=\"http:\/\/historymatters.gmu.edu\/d\/6447\/\">Don&#8217;t smoke &#8211; unless you like it.<\/a> <em>Collier&#8217;s,<\/em> November 4, 1950, 18.<\/li>\n<li>Meyer, J. A. (1992). Cigarette century. <em>American Heritage Magazine, 43<\/em>(8).<\/li>\n<li>NCI &#8211; National Cancer Institute (1997). Changes in cigarette-related disease risks and their implications for prevention and control. <em>Smoking and Tobacco Control Monographs,<\/em> National Institutes of Health.<\/li>\n<li>Newman, C. (1975). Edward Tyson. <em>British Medical Journal 4,<\/em> 96-97.<\/li>\n<li>Roberts, L. (1989). Global warming: Blaming the sun. <em>Science 246<\/em>(4933), 992-93.<\/li>\n<li>Russell, K. F. (1967). Edward Tyson&#8217;s <em>Orang-Outang. Medical History, 11<\/em>(4), 417-423.<\/li>\n<li>Salsburg, D. (2001). <em>The lady tasting tea: How statistics revolutionized science in the twentieth century.<\/em> New York: W. H. Freeman &amp; Company.<\/li>\n<li>Witschi, H. (2001). A short history of lung cancer. <em>Toxicological Sciences 4<\/em>(1), 4-6.<\/li>\n<li>Wynder, E. L., &amp; Graham, E. A. (1950). <a href=\"http:\/\/tobacco.neu.edu\/box\/BOEKENBox\/medicalarticles.html\">Tobacco smoking as a possible etiological factor in bronchiogenic carcinoma<\/a>. <em>Journal of the American Medical Association 143<\/em>(4), 329-338.<\/li>\n<\/ul>\n<\/footer>\n","protected":false},"author":51812,"menu_order":7,"template":"","meta":{"_candela_citation":"[]","CANDELA_OUTCOMES_GUID":"","pb_show_title":"on","pb_short_title":"","pb_subtitle":"","pb_authors":[],"pb_section_license":""},"chapter-type":[],"contributor":[],"license":[],"class_list":["post-87","chapter","type-chapter","status-publish","hentry"],"part":49,"_links":{"self":[{"href":"https:\/\/courses.lumenlearning.com\/suny-hccc-generalscience\/wp-json\/pressbooks\/v2\/chapters\/87","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/courses.lumenlearning.com\/suny-hccc-generalscience\/wp-json\/pressbooks\/v2\/chapters"}],"about":[{"href":"https:\/\/courses.lumenlearning.com\/suny-hccc-generalscience\/wp-json\/wp\/v2\/types\/chapter"}],"author":[{"embeddable":true,"href":"https:\/\/courses.lumenlearning.com\/suny-hccc-generalscience\/wp-json\/wp\/v2\/users\/51812"}],"version-history":[{"count":1,"href":"https:\/\/courses.lumenlearning.com\/suny-hccc-generalscience\/wp-json\/pressbooks\/v2\/chapters\/87\/revisions"}],"predecessor-version":[{"id":88,"href":"https:\/\/courses.lumenlearning.com\/suny-hccc-generalscience\/wp-json\/pressbooks\/v2\/chapters\/87\/revisions\/88"}],"part":[{"href":"https:\/\/courses.lumenlearning.com\/suny-hccc-generalscience\/wp-json\/pressbooks\/v2\/parts\/49"}],"metadata":[{"href":"https:\/\/courses.lumenlearning.com\/suny-hccc-generalscience\/wp-json\/pressbooks\/v2\/chapters\/87\/metadata\/"}],"wp:attachment":[{"href":"https:\/\/courses.lumenlearning.com\/suny-hccc-generalscience\/wp-json\/wp\/v2\/media?parent=87"}],"wp:term":[{"taxonomy":"chapter-type","embeddable":true,"href":"https:\/\/courses.lumenlearning.com\/suny-hccc-generalscience\/wp-json\/pressbooks\/v2\/chapter-type?post=87"},{"taxonomy":"contributor","embeddable":true,"href":"https:\/\/courses.lumenlearning.com\/suny-hccc-generalscience\/wp-json\/wp\/v2\/contributor?post=87"},{"taxonomy":"license","embeddable":true,"href":"https:\/\/courses.lumenlearning.com\/suny-hccc-generalscience\/wp-json\/wp\/v2\/license?post=87"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}