{"id":87,"date":"2018-12-19T14:33:14","date_gmt":"2018-12-19T14:33:14","guid":{"rendered":"https:\/\/courses.lumenlearning.com\/suny-microbio-labexperience\/back-matter\/appendix-c\/"},"modified":"2018-12-19T14:33:14","modified_gmt":"2018-12-19T14:33:14","slug":"appendix-c","status":"publish","type":"back-matter","link":"https:\/\/courses.lumenlearning.com\/suny-microbio-labexperience\/back-matter\/appendix-c\/","title":{"raw":"Appendix C","rendered":"Appendix C"},"content":{"raw":"\n

Differentiation of Bacterial Cultures based on Morphological and Physiological Characteristics<\/h2>\n

Group 1: Aerobic and Anaerobic Cocci<\/h3>\n\n\n\n\n<\/colgroup>\n\n\n\n\n\n
Table 1. Aerobic cocci.<\/strong><\/td>\n<\/tr>\n
<\/td>\nGram stain<\/td>\nArrangement<\/td>\n<\/tr>\n
Micrococcus<\/i> spp.<\/i><\/td>\n+<\/td>\ntetrads<\/td>\n<\/tr>\n
Neisseria gonorrhoeae<\/i><\/td>\n-<\/td>\ndiplococci<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n\n\n<\/colgroup>\n\n\n\n\n\n\n
Table 2. Anaerobic cocci.<\/strong><\/td>\n<\/tr>\n
<\/td>\nCatalase<\/td>\nOxidase<\/td>\nArrangement<\/td>\nOxygen<\/td>\n<\/tr>\n
Staphylococcus<\/i> spp (see Table 1)<\/td>\n+<\/td>\n-<\/td>\nclusters<\/td>\nFacultative anaerobe<\/td>\n<\/tr>\n
Streptococcus <\/i>spp.<\/i>\n

(see Table 4)<\/p><\/td>\n

-<\/td>\n-<\/td>\nchains<\/td>\nAerotolerant anaerobe<\/td>\n<\/tr>\n
Enterococcus <\/i>spp.<\/i>\n

(see Table 4)<\/p><\/td>\n

-<\/td>\n-<\/td>\nchains<\/td>\nAerotolerant anaerobe<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n\n<\/colgroup>\n\n\n\n\n\n\n\n\n
Table 3. Differentiation of Staphylococcus<\/i> spp.<\/strong><\/td>\n<\/tr>\n
<\/td>\nStaphylococcus aureus<\/i><\/td>\nStaphylococcus epidermidis<\/i><\/td>\nStaphylococcus saprophyticus<\/i><\/td>\n<\/tr>\n
Coagulase<\/td>\n4+<\/td>\n-<\/td>\n1\u20132+<\/td>\n<\/tr>\n
Mannitol Salt Agar<\/td>\n+<\/td>\n-<\/td>\n+<\/td>\n<\/tr>\n
Urease<\/td>\n- or wk+<\/td>\n+<\/td>\n+<\/td>\n<\/tr>\n
Hemolysis<\/td>\nBeta (\u03b2)<\/td>\n- or wk +<\/td>\n-<\/td>\n<\/tr>\n
Novobiocin resistance<\/td>\nSusc. (-)<\/td>\nSusc. (-)<\/td>\nRes. (+)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n<\/colgroup>\n\n\n\n\n\n
Table 4. Differentiation of Streptococcus <\/i>and Enterococcus <\/i>spp.<\/i><\/strong><\/i><\/td>\n<\/tr>\n
<\/td>\nStreptococcus pyogenes<\/i><\/td>\nEnterococcus faecalis<\/i><\/td>\n<\/tr>\n
Hemolysis on BAP<\/td>\nBeta (\u03b2)<\/td>\nGamma (\u03b3)<\/td>\n<\/tr>\n
Bile Esculin<\/td>\nNo growth (-)<\/td>\nGrowth\/black (+)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Group 2: Aerobic and Facultatively Anaerobic Bacilli<\/h3>\n

Gram positive bacilli<\/b>\n

Endospore forming: Bacillus<\/i> spp. (see Table 5)<\/p>\n

Non-endospore forming: Corynebacterium xerosis<\/i><\/i>\n\n\n\n<\/colgroup>\n\n\n\n\n\n
Table 5. <\/i>Differentiation of Bacillus<\/i> spp.<\/strong><\/td>\n<\/tr>\n
<\/td>\nEndospore shape, location, sporangium<\/td>\n<\/tr>\n
B. cereus<\/i><\/td>\nOval, central, sporangium not swollen<\/td>\n<\/tr>\n
B. <\/i>lentus<\/i><\/td>\nOval, terminal, sporangium swollen<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Gram negative bacilli<\/b>\n

<\/i>Strictly aerobic: Pseudomonas aeruginosa<\/i>\n

<\/i>Facultatively anaerobic: Enterobacteriaceae (see Table 6)<\/p>\n\n\n\n\n\n\n\n<\/colgroup>\n\n\n\n\n\n\n\n\n
Table 6. Differentiation of Enterobacteriaceae<\/strong><\/td>\n<\/tr>\n
<\/td>\nTSI<\/td>\nSulfide\/Indole\/Motility<\/td>\nMethyl Red<\/td>\nVP<\/td>\nCitrate<\/td>\n<\/tr>\n
Escherichia coli<\/i><\/td>\nA\/A<\/td>\n- \/+\/+<\/td>\n+<\/td>\n-<\/td>\n-<\/td>\n<\/tr>\n
Enterobacter aerogenes<\/i><\/td>\nA\/A<\/td>\n-\/-\/+<\/td>\n-<\/td>\n+<\/td>\n+<\/td>\n<\/tr>\n
Citrobacter freundii<\/i><\/td>\nA\/A<\/td>\n-\/-\/+<\/td>\n+<\/td>\n-<\/td>\n+<\/td>\n<\/tr>\n
Klebsiella pneumoniae<\/i><\/td>\nA\/A<\/td>\n-\/-\/-<\/td>\n-<\/td>\n+<\/td>\n+<\/td>\n<\/tr>\n
Salmonella enteritidis<\/i><\/td>\nK\/A<\/td>\n+\/-\/+<\/td>\n+<\/td>\n-<\/td>\n+<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n","rendered":"

Differentiation of Bacterial Cultures based on Morphological and Physiological Characteristics<\/h2>\n

Group 1: Aerobic and Anaerobic Cocci<\/h3>\n\n\n\n\n<\/colgroup>\n\n\n\n\n\n
Table 1. Aerobic cocci.<\/strong><\/td>\n<\/tr>\n
<\/td>\nGram stain<\/td>\nArrangement<\/td>\n<\/tr>\n
Micrococcus<\/i> spp.<\/i><\/td>\n+<\/td>\ntetrads<\/td>\n<\/tr>\n
Neisseria gonorrhoeae<\/i><\/td>\n–<\/td>\ndiplococci<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n\n\n<\/colgroup>\n\n\n\n\n\n\n
Table 2. Anaerobic cocci.<\/strong><\/td>\n<\/tr>\n
<\/td>\nCatalase<\/td>\nOxidase<\/td>\nArrangement<\/td>\nOxygen<\/td>\n<\/tr>\n
Staphylococcus<\/i> spp (see Table 1)<\/td>\n+<\/td>\n–<\/td>\nclusters<\/td>\nFacultative anaerobe<\/td>\n<\/tr>\n
Streptococcus <\/i>spp.<\/i><\/p>\n

(see Table 4)<\/p>\n<\/td>\n

–<\/td>\n–<\/td>\nchains<\/td>\nAerotolerant anaerobe<\/td>\n<\/tr>\n
Enterococcus <\/i>spp.<\/i><\/p>\n

(see Table 4)<\/p>\n<\/td>\n

–<\/td>\n–<\/td>\nchains<\/td>\nAerotolerant anaerobe<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n\n<\/colgroup>\n\n\n\n\n\n\n\n\n
Table 3. Differentiation of Staphylococcus<\/i> spp.<\/strong><\/td>\n<\/tr>\n
<\/td>\nStaphylococcus aureus<\/i><\/td>\nStaphylococcus epidermidis<\/i><\/td>\nStaphylococcus saprophyticus<\/i><\/td>\n<\/tr>\n
Coagulase<\/td>\n4+<\/td>\n–<\/td>\n1\u20132+<\/td>\n<\/tr>\n
Mannitol Salt Agar<\/td>\n+<\/td>\n–<\/td>\n+<\/td>\n<\/tr>\n
Urease<\/td>\n– or wk+<\/td>\n+<\/td>\n+<\/td>\n<\/tr>\n
Hemolysis<\/td>\nBeta (\u03b2)<\/td>\n– or wk +<\/td>\n–<\/td>\n<\/tr>\n
Novobiocin resistance<\/td>\nSusc. (-)<\/td>\nSusc. (-)<\/td>\nRes. (+)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n<\/colgroup>\n\n\n\n\n\n
Table 4. Differentiation of Streptococcus <\/i>and Enterococcus <\/i>spp.<\/i><\/strong><\/i><\/td>\n<\/tr>\n
<\/td>\nStreptococcus pyogenes<\/i><\/td>\nEnterococcus faecalis<\/i><\/td>\n<\/tr>\n
Hemolysis on BAP<\/td>\nBeta (\u03b2)<\/td>\nGamma (\u03b3)<\/td>\n<\/tr>\n
Bile Esculin<\/td>\nNo growth (-)<\/td>\nGrowth\/black (+)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Group 2: Aerobic and Facultatively Anaerobic Bacilli<\/h3>\n

Gram positive bacilli<\/b>\n<\/p>\n

Endospore forming: Bacillus<\/i> spp. (see Table 5)<\/p>\n

Non-endospore forming: Corynebacterium xerosis<\/i><\/i>\n<\/p>\n\n\n\n<\/colgroup>\n\n\n\n\n\n
Table 5. <\/i>Differentiation of Bacillus<\/i> spp.<\/strong><\/td>\n<\/tr>\n
<\/td>\nEndospore shape, location, sporangium<\/td>\n<\/tr>\n
B. cereus<\/i><\/td>\nOval, central, sporangium not swollen<\/td>\n<\/tr>\n
B. <\/i>lentus<\/i><\/td>\nOval, terminal, sporangium swollen<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Gram negative bacilli<\/b>\n<\/p>\n

<\/i>Strictly aerobic: Pseudomonas aeruginosa<\/i>\n<\/p>\n

<\/i>Facultatively anaerobic: Enterobacteriaceae (see Table 6)<\/p>\n\n\n\n\n\n\n\n<\/colgroup>\n\n\n\n\n\n\n\n\n
Table 6. Differentiation of Enterobacteriaceae<\/strong><\/td>\n<\/tr>\n
<\/td>\nTSI<\/td>\nSulfide\/Indole\/Motility<\/td>\nMethyl Red<\/td>\nVP<\/td>\nCitrate<\/td>\n<\/tr>\n
Escherichia coli<\/i><\/td>\nA\/A<\/td>\n– \/+\/+<\/td>\n+<\/td>\n–<\/td>\n–<\/td>\n<\/tr>\n
Enterobacter aerogenes<\/i><\/td>\nA\/A<\/td>\n-\/-\/+<\/td>\n–<\/td>\n+<\/td>\n+<\/td>\n<\/tr>\n
Citrobacter freundii<\/i><\/td>\nA\/A<\/td>\n-\/-\/+<\/td>\n+<\/td>\n–<\/td>\n+<\/td>\n<\/tr>\n
Klebsiella pneumoniae<\/i><\/td>\nA\/A<\/td>\n-\/-\/-<\/td>\n–<\/td>\n+<\/td>\n+<\/td>\n<\/tr>\n
Salmonella enteritidis<\/i><\/td>\nK\/A<\/td>\n+\/-\/+<\/td>\n+<\/td>\n–<\/td>\n+<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\t\t\t
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Candela Citations<\/h3>\n\t\t\t\t\t
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