{"id":761,"date":"2018-05-03T18:12:19","date_gmt":"2018-05-03T18:12:19","guid":{"rendered":"https:\/\/courses.lumenlearning.com\/suny-osbiology2e\/chapter\/cell-division-2\/"},"modified":"2018-06-27T18:44:02","modified_gmt":"2018-06-27T18:44:02","slug":"cell-division-2","status":"publish","type":"chapter","link":"https:\/\/courses.lumenlearning.com\/suny-osbiology2e\/chapter\/cell-division-2\/","title":{"raw":"Cell Division","rendered":"Cell Division"},"content":{"raw":"<div class=\"textbox learning-objectives\">\r\n<h3>Learning Objectives<\/h3>\r\nBy the end of this section, you will be able to do the following:\r\n<ul>\r\n \t<li>Describe the structure of prokaryotic and eukaryotic genomes<\/li>\r\n \t<li>Distinguish between chromosomes, genes, and traits<\/li>\r\n \t<li>Describe the mechanisms of chromosome compaction<\/li>\r\n<\/ul>\r\n<\/div>\r\n<p id=\"fs-id2023520\">The continuity of life from one cell to another has its foundation in the reproduction of cells by way of the cell cycle. The cell cycle is an orderly sequence of events that describes the stages of a cell\u2019s life from the division of a single parent cell to the production of two new genetically identical daughter cells.<\/p>\r\n\r\n<div id=\"fs-id1235767\" class=\"bc-section section\">\r\n<h3>Genomic DNA<\/h3>\r\nBefore discussing the steps a cell must undertake to replicate and divide its DNA, a deeper understanding of the structure and function of a cell\u2019s genetic information is necessary. A cell\u2019s DNA, packaged as a double-stranded DNA molecule, is called its genome. In prokaryotes, the genome is composed of a single, double-stranded DNA molecule in the form of a loop or circle (<a class=\"autogenerated-content\" href=\"#fig-ch10_01_01\">(Figure)<\/a>). The region in the cell containing this genetic material is called a nucleoid. Some prokaryotes also have smaller loops of DNA called plasmids that are not essential for normal growth. Bacteria can exchange these plasmids with other bacteria, sometimes receiving beneficial new genes that the recipient can add to their chromosomal DNA. <em>Antibiotic resistance<\/em> is one trait that often spreads through a bacterial colony through plasmid exchange from resistant donors to recipient cells.\r\n<div id=\"fig-ch10_01_01\" class=\"wp-caption aligncenter\">\r\n\r\n[caption id=\"\" align=\"aligncenter\" width=\"320\"]<img src=\"https:\/\/s3-us-west-2.amazonaws.com\/courses-images\/wp-content\/uploads\/sites\/3206\/2018\/05\/03181211\/Figure_10_01_01.jpg\" alt=\"The illustration shows a prokaryotic cell with a single, circular chromosome floating free in the cytoplasm.\" width=\"320\" height=\"381\" \/> <strong>Figure 1. <\/strong>Prokaryotes, including both Bacteria and Archaea, have a single, circular chromosome located in a central region called the nucleoid.[\/caption]\r\n\r\n<\/div>\r\n<p id=\"fs-id2116400\">In eukaryotes, the genome consists of several double-stranded linear DNA molecules (<a class=\"autogenerated-content\" href=\"#fig-ch10_01_02\">(Figure)<\/a>). Each species of eukaryotes has a characteristic number of chromosomes in the nuclei of its cells. Human body (somatic) cells have 46 chromosomes, while human gametes (sperm or eggs) have 23 chromosomes each. A typical body cell contains two matched or homologous sets of chromosomes (one set from each biological parent)\u2014a configuration known as diploid. (Note: The letter <em>n<\/em> is used to represent a single set of chromosomes; therefore, a diploid organism is designated 2<em>n<\/em>.) Human cells that contain one set of chromosomes are called gametes, or sex cells; these are eggs and sperm, and are designated <em>1n<\/em>, or haploid.<\/p>\r\nUpon fertilization, each gamete contributes one set of chromosomes, creating a diploid cell containing matched pairs of chromosomes called homologous (\u201csame knowledge\u201d) chromosomes. Homologous chromosomes are the same length and have specific nucleotide segments called genes in exactly the same location, or locus. Genes, the functional units of chromosomes, determine specific characteristics by coding for specific proteins. Traits are the variations of those characteristics. For example, hair color is a characteristic with traits that are blonde, brown, or black, and many colors in between.\r\n<div id=\"fig-ch10_01_02\" class=\"wp-caption aligncenter\">\r\n\r\n[caption id=\"\" align=\"aligncenter\" width=\"320\"]<img src=\"https:\/\/s3-us-west-2.amazonaws.com\/courses-images\/wp-content\/uploads\/sites\/3206\/2018\/05\/03181214\/Figure_10_01_02.jpg\" alt=\"The 23 chromosomes from a human female are each dyed a different color so they can be distinguished. During most of the cell cycle, each chromosome is elongated into a thin strand that folds over on itself, like a piece of spaghetti. The chromosomes fill the entire spherical nucleus, but each one is contained in a different part, resulting in a multi-colored sphere. During mitosis, the chromosomes condense into thick, compact bars, each a different color. These bars can be arranged in numerical order to form a karyotype. There are two copies of each chromosome in the karyotype..\" width=\"320\" height=\"430\" \/> <strong>Figure 2. <\/strong>There are 23 pairs of homologous chromosomes in a female human somatic cell. The condensed chromosomes are viewed within the nucleus (top), removed from a cell during mitosis (also called karyokinesis or nuclear division) and spread out on a slide (right), and artificially arranged according to length (left); an arrangement like this is called a karyotype. In this image, the chromosomes were exposed to fluorescent stains for differentiation of the different chromosomes. A method of staining called \u201cchromosome painting\u201d employs fluorescent dyes that highlight chromosomes in different colors. (credit: National Human Genome Project\/NIH)[\/caption]\r\n\r\n<\/div>\r\n<p id=\"fs-id2048187\">Each copy of a homologous pair of chromosomes originates from a different parent; therefore, the different genes (alleles) themselves are not identical, although they code for the same traits such as \u201chair color.\u201d The variation of individuals within a species is due to the specific combination of the genes inherited from both parents. Even a slightly altered sequence of nucleotides within a gene can result in an alternative trait. For example, there are three possible gene sequences on the human chromosome that code for blood type: sequence A, sequence B, and sequence O. Because all diploid human cells have two copies of the chromosome that determines blood type, the blood type (the trait) is determined by the two alleles of the marker gene that are inherited. It is possible to have two copies of the same gene sequence on both homologous chromosomes, with one on each (for example, AA, BB, or OO), or two different sequences, such as AB, AO, or BO.<\/p>\r\nApparently minor variations of traits, such as blood type, eye color, and handedness, contribute to the natural variation found within a species, but even though they seem minor, these traits may be connected with the expression of other traits as of yet unknown. However, if the entire DNA sequence from any pair of human homologous chromosomes is compared, the difference is much less than one percent. The sex chromosomes, X and Y, are the single exception to the rule of homologous chromosome uniformity: Other than a small amount of homology that is necessary to accurately produce gametes, the genes found on the X and Y chromosomes are different.\r\n\r\n<\/div>\r\n<div class=\"bc-section section\">\r\n<h3>Eukaryotic Chromosomal Structure and Compaction<\/h3>\r\n<p id=\"fs-id2385346\">If the DNA from all 46 chromosomes in a human cell nucleus were laid out end-to-end, it would measure approximately two meters; however, its diameter would be only 2 nm! Considering that the size of a typical human cell is about 10 \u00b5m (100,000 cells lined up to equal one meter), DNA must be <em>tightly packaged<\/em> to fit in the cell\u2019s nucleus. At the same time, it must also be readily accessible for the genes to be expressed. For this reason, the long strands of DNA are condensed into compact chromosomes during certain stages of the cell cycle. There are a number of ways that chromosomes are compacted.<\/p>\r\n<p id=\"fs-id1393700\">In the first level of compaction, short stretches of the DNA double helix wrap around a core of eight histone proteins at regular intervals along the entire length of the chromosome (<a class=\"autogenerated-content\" href=\"#fig-ch10_01_03\">(Figure)<\/a>). The DNA-histone complex is called chromatin. The beadlike, histone DNA complex is called a nucleosome, and DNA connecting the nucleosomes is called linker DNA. A DNA molecule in this form is about seven times shorter than the double helix without the histones, and the beads are about 10 nm in diameter, in contrast with the 2-nm diameter of a DNA double helix.<\/p>\r\n<p id=\"fs-id1373700\">The second level of compaction occurs as the nucleosomes and the linker DNA between them coil into a 30-nm chromatin fiber. This coiling further <em>condenses<\/em> the chromosome so that it is now about 50 times shorter than the extended form.<\/p>\r\n<p id=\"fs-id1373701\">In the third level of compaction, a variety of <em>fibrous proteins<\/em> is used to \u201cpack the chromatin.\u201d These fibrous proteins also ensure that each chromosome in a non-dividing cell occupies a particular area of the nucleus that does not overlap with that of any other chromosome (see the top image in <a class=\"autogenerated-content\" href=\"#fig-ch10_01_02\">(Figure)<\/a>).<\/p>\r\n\r\n<div id=\"fig-ch10_01_03\" class=\"wp-caption aligncenter\">\r\n\r\n[caption id=\"\" align=\"aligncenter\" width=\"320\"]<img src=\"https:\/\/s3-us-west-2.amazonaws.com\/courses-images\/wp-content\/uploads\/sites\/3206\/2018\/05\/03181217\/Figure_10_01_03.jpg\" alt=\"There are five levels of chromosome organization. From top to bottom: The top panel shows a DNA double helix. The second panel shows the double helix wrapped around proteins called histones. The middle panel shows the entire DNA molecule wrapping around many histones, creating the appearance of beads on a string. The fourth panel shows that the chromatin fiber further condenses into the chromosome shown in the bottom panel.\" width=\"320\" height=\"939\" \/> <strong>Figure 3. <\/strong>Double-stranded DNA wraps around histone proteins to form nucleosomes that create the appearance of \u201cbeads on a string.\u201d The nucleosomes are coiled into a 30-nm chromatin fiber. When a cell undergoes mitosis, the chromosomes condense even further.[\/caption]\r\n\r\n<\/div>\r\n<p id=\"fs-id1466552\"><em>DNA replicates in the S phase of interphase, which technically is not a part of mitosis, but must always precede it<\/em>. After replication, the chromosomes are composed of two linked sister chromatids. When fully compact, the pairs of identically packed chromosomes are bound to each other by cohesin proteins. The connection between the sister chromatids is closest in a region called the centromere. The conjoined sister chromatids, with a diameter of about 1 \u00b5m, are visible under a light microscope. The centromeric region is highly condensed and thus will appear as a constricted area.<\/p>\r\n\r\n<div id=\"fs-id2919212\" class=\"interactive textbox tryit\">\r\n<h3>Link to Learning<\/h3>\r\n<p id=\"fs-id2338602\">This <a href=\"https:\/\/youtu.be\/gbSIBhFwQ4s\">animation<\/a> illustrates the different levels of chromosome packing.<\/p>\r\n\r\n<\/div>\r\n<\/div>\r\n<div class=\"summary textbox key-takeaways\">\r\n<h3>Section Summary<\/h3>\r\nProkaryotes have a single circular chromosome composed of double-stranded DNA, whereas eukaryotes have multiple, linear chromosomes composed of chromatin wrapped around histones, all of which are surrounded by a nuclear membrane. The 46 chromosomes of human somatic cells are composed of 22 pairs of autosomes (matched pairs) and a pair of sex chromosomes, which may or may not be matched. This is the 2<em>n<\/em> or diploid state. Human gametes have 23 chromosomes, or one complete set of chromosomes; a set of chromosomes is complete with either one of the sex chromosomes, X or Y. This is the <em>n<\/em> or haploid state. Genes are segments of DNA that code for a specific functional molecule (a protein or RNA). An organism\u2019s traits are determined by the genes inherited from each parent. Duplicated chromosomes are composed of two sister chromatids. Chromosomes are compacted using a variety of mechanisms during certain stages of the cell cycle. Several classes of protein are involved in the organization and packing of the chromosomal DNA into a highly condensed structure. The condensing complex compacts chromosomes, and the resulting condensed structure is necessary for chromosomal segregation during mitosis.\r\n\r\n<\/div>\r\n<div id=\"fs-id1569634\" class=\"multiple-choice textbox exercises\">\r\n<h3>Review Questions<\/h3>\r\n<div>\r\n<div id=\"fs-id1419233\">\r\n<p id=\"fs-id2641842\">A diploid cell has_______ the number of chromosomes as a haploid cell.<\/p>\r\n\r\n<ol id=\"fs-id2491390\" type=\"a\">\r\n \t<li>one-fourth<\/li>\r\n \t<li>half<\/li>\r\n \t<li>twice<\/li>\r\n \t<li>four times<\/li>\r\n<\/ol>\r\n<\/div>\r\n[reveal-answer q=\"fs-id1982850\"]Show Solution[\/reveal-answer]\r\n[hidden-answer a=\"fs-id1982850\"]\r\n<div id=\"fs-id1982850\">\r\n<p id=\"fs-id2909422\">C<\/p>\r\n\r\n<\/div>\r\n[\/hidden-answer]\r\n\r\n<\/div>\r\n<div>\r\n<div id=\"fs-id3241208\">\r\n<p id=\"fs-id2335848\">An organism\u2019s traits are determined by the specific combination of inherited _____.<\/p>\r\n\r\n<ol id=\"fs-id2077209\" type=\"a\">\r\n \t<li>cells.<\/li>\r\n \t<li>genes.<\/li>\r\n \t<li>proteins.<\/li>\r\n \t<li>chromatids.<\/li>\r\n<\/ol>\r\n<\/div>\r\n[reveal-answer q=\"fs-id1293208\"]Show Solution[\/reveal-answer]\r\n[hidden-answer a=\"fs-id1293208\"]\r\n<div id=\"fs-id1293208\">\r\n<p id=\"fs-id2642965\">B<\/p>\r\n\r\n<\/div>\r\n[\/hidden-answer]\r\n\r\n<\/div>\r\n<div>\r\n<div id=\"fs-id1447690\">\r\n<p id=\"fs-id2060998\">The first level of DNA organization in a eukaryotic cell is maintained by which molecule?<\/p>\r\n\r\n<ol id=\"fs-id2165232\" type=\"a\">\r\n \t<li>cohesin<\/li>\r\n \t<li>condensin<\/li>\r\n \t<li>chromatin<\/li>\r\n \t<li>histone<\/li>\r\n<\/ol>\r\n<\/div>\r\n[reveal-answer q=\"fs-id2186440\"]Show Solution[\/reveal-answer]\r\n[hidden-answer a=\"fs-id2186440\"]\r\n<div id=\"fs-id2186440\">\r\n<p id=\"fs-id1975120\">D<\/p>\r\n\r\n<\/div>\r\n[\/hidden-answer]\r\n\r\n<\/div>\r\n<div id=\"fs-id2315550\">\r\n<div id=\"fs-id1853884\">\r\n\r\nIdentical copies of chromatin held together by cohesin at the centromere are called _____.\r\n<ol type=\"a\">\r\n \t<li>histones.<\/li>\r\n \t<li>nucleosomes.<\/li>\r\n \t<li>chromatin.<\/li>\r\n \t<li>sister chromatids.<\/li>\r\n<\/ol>\r\n<\/div>\r\n[reveal-answer q=\"fs-id1711955\"]Show Solution[\/reveal-answer]\r\n[hidden-answer a=\"fs-id1711955\"]\r\n<div id=\"fs-id1711955\">\r\n<p id=\"fs-id1651050\">D<\/p>\r\n\r\n<\/div>\r\n[\/hidden-answer]\r\n\r\n<\/div>\r\n<\/div>\r\n<div class=\"free-response textbox exercises\">\r\n<h3>Free Response<\/h3>\r\n<div>\r\n<div>\r\n\r\nCompare and contrast a human somatic cell to a human gamete.\r\n\r\n<\/div>\r\n[reveal-answer q=\"fs-id3014809\"]Show Solution[\/reveal-answer]\r\n[hidden-answer a=\"fs-id3014809\"]\r\n<div id=\"fs-id3014809\">\r\n<p id=\"fs-id2187324\">Human somatic cells have 46 chromosomes: 22 pairs and 2 sex chromosomes that may or may not form a pair. This is the 2<em>n<\/em> or diploid condition. Human gametes have 23 chromosomes, one each of 23 unique chromosomes, one of which is a sex chromosome. This is the <em>n<\/em> or haploid condition.<\/p>\r\n\r\n<\/div>\r\n[\/hidden-answer]\r\n\r\n<\/div>\r\n<div>\r\n<div id=\"fs-id1794020\">\r\n\r\nWhat is the relationship between a genome, chromosomes, and genes?\r\n\r\n<\/div>\r\n[reveal-answer q=\"fs-id2739844\"]Show Solution[\/reveal-answer]\r\n[hidden-answer a=\"fs-id2739844\"]\r\n<div id=\"fs-id2739844\">\r\n\r\nThe genome consists of the sum total of an organism\u2019s chromosomes. Each chromosome contains hundreds and sometimes thousands of genes, segments of DNA that code for a polypeptide or RNA, and a large amount of DNA with no known function.\r\n\r\n<\/div>\r\n[\/hidden-answer]\r\n\r\n<\/div>\r\n<div id=\"fs-id1444370\">\r\n<div id=\"fs-id1595608\">\r\n<p id=\"fs-id1468864\">Eukaryotic chromosomes are thousands of times longer than a typical cell. Explain how chromosomes can fit inside a eukaryotic nucleus.<\/p>\r\n\r\n<\/div>\r\n[reveal-answer q=\"fs-id2643307\"]Show Solution[\/reveal-answer]\r\n[hidden-answer a=\"fs-id2643307\"]\r\n<div id=\"fs-id2643307\">\r\n\r\nThe DNA double helix is wrapped around histone proteins to form structures called nucleosomes. Nucleosomes and the linker DNA in between them are coiled into a 30-nm fiber. During cell division, chromatin is further condensed by packing proteins.\r\n\r\n<\/div>\r\n[\/hidden-answer]\r\n\r\n<\/div>\r\n<\/div>\r\n<div class=\"textbox shaded\">\r\n<h3>Glossary<\/h3>\r\n<dl id=\"fs-id2216573\">\r\n \t<dt>cell cycle<\/dt>\r\n \t<dd id=\"fs-id1293714\">ordered sequence of events through which a cell passes between one cell division and the next<\/dd>\r\n<\/dl>\r\n<dl>\r\n \t<dt>centromere<\/dt>\r\n \t<dd id=\"fs-id1626303\">region at which sister chromatids are bound together; a constricted area in condensed chromosomes<\/dd>\r\n<\/dl>\r\n<dl id=\"fs-id2200988\">\r\n \t<dt>chromatid<\/dt>\r\n \t<dd>single DNA molecule of two strands of duplicated DNA and associated proteins held together at the centromere<\/dd>\r\n<\/dl>\r\n<dl>\r\n \t<dt>diploid<\/dt>\r\n \t<dd>cell, nucleus, or organism containing two sets of chromosomes (2<em>n<\/em>)<\/dd>\r\n<\/dl>\r\n<dl id=\"fs-id1693570\">\r\n \t<dt>gamete<\/dt>\r\n \t<dd id=\"fs-id1976449\">haploid reproductive cell or sex cell (sperm, pollen grain, or egg)<\/dd>\r\n<\/dl>\r\n<dl id=\"fs-id2121017\">\r\n \t<dt>gene<\/dt>\r\n \t<dd id=\"fs-id2011748\">physical and functional unit of heredity, a sequence of DNA that codes for a protein.<\/dd>\r\n<\/dl>\r\n<dl id=\"fs-id2150211\">\r\n \t<dt>genome<\/dt>\r\n \t<dd id=\"fs-id1453713\">total genetic information of a cell or organism<\/dd>\r\n<\/dl>\r\n<dl id=\"fs-id1730718\">\r\n \t<dt>haploid<\/dt>\r\n \t<dd id=\"fs-id1569099\">cell, nucleus, or organism containing one set of chromosomes (<em>n<\/em>)<\/dd>\r\n<\/dl>\r\n<dl id=\"fs-id2654173\">\r\n \t<dt>histone<\/dt>\r\n \t<dd id=\"fs-id2569081\">one of several similar, highly conserved, low molecular weight, basic proteins found in the chromatin of all eukaryotic cells; associates with DNA to form nucleosomes<\/dd>\r\n<\/dl>\r\n<dl>\r\n \t<dt>homologous chromosomes<\/dt>\r\n \t<dd id=\"fs-id1791301\">chromosomes of the same morphology with genes in the same location; diploid organisms have pairs of homologous chromosomes (homologs), with each homolog derived from a different parent<\/dd>\r\n<\/dl>\r\n<dl>\r\n \t<dt>locus<\/dt>\r\n \t<dd id=\"fs-id2626368\">position of a gene on a chromosome<\/dd>\r\n<\/dl>\r\n<dl>\r\n \t<dt>nucleosome<\/dt>\r\n \t<dd id=\"fs-id3032152\">subunit of chromatin composed of a short length of DNA wrapped around a core of histone proteins<\/dd>\r\n<\/dl>\r\n<\/div>","rendered":"<div class=\"textbox learning-objectives\">\n<h3>Learning Objectives<\/h3>\n<p>By the end of this section, you will be able to do the following:<\/p>\n<ul>\n<li>Describe the structure of prokaryotic and eukaryotic genomes<\/li>\n<li>Distinguish between chromosomes, genes, and traits<\/li>\n<li>Describe the mechanisms of chromosome compaction<\/li>\n<\/ul>\n<\/div>\n<p id=\"fs-id2023520\">The continuity of life from one cell to another has its foundation in the reproduction of cells by way of the cell cycle. The cell cycle is an orderly sequence of events that describes the stages of a cell\u2019s life from the division of a single parent cell to the production of two new genetically identical daughter cells.<\/p>\n<div id=\"fs-id1235767\" class=\"bc-section section\">\n<h3>Genomic DNA<\/h3>\n<p>Before discussing the steps a cell must undertake to replicate and divide its DNA, a deeper understanding of the structure and function of a cell\u2019s genetic information is necessary. A cell\u2019s DNA, packaged as a double-stranded DNA molecule, is called its genome. In prokaryotes, the genome is composed of a single, double-stranded DNA molecule in the form of a loop or circle (<a class=\"autogenerated-content\" href=\"#fig-ch10_01_01\">(Figure)<\/a>). The region in the cell containing this genetic material is called a nucleoid. Some prokaryotes also have smaller loops of DNA called plasmids that are not essential for normal growth. Bacteria can exchange these plasmids with other bacteria, sometimes receiving beneficial new genes that the recipient can add to their chromosomal DNA. <em>Antibiotic resistance<\/em> is one trait that often spreads through a bacterial colony through plasmid exchange from resistant donors to recipient cells.<\/p>\n<div id=\"fig-ch10_01_01\" class=\"wp-caption aligncenter\">\n<div style=\"width: 330px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/s3-us-west-2.amazonaws.com\/courses-images\/wp-content\/uploads\/sites\/3206\/2018\/05\/03181211\/Figure_10_01_01.jpg\" alt=\"The illustration shows a prokaryotic cell with a single, circular chromosome floating free in the cytoplasm.\" width=\"320\" height=\"381\" \/><\/p>\n<p class=\"wp-caption-text\"><strong>Figure 1. <\/strong>Prokaryotes, including both Bacteria and Archaea, have a single, circular chromosome located in a central region called the nucleoid.<\/p>\n<\/div>\n<\/div>\n<p id=\"fs-id2116400\">In eukaryotes, the genome consists of several double-stranded linear DNA molecules (<a class=\"autogenerated-content\" href=\"#fig-ch10_01_02\">(Figure)<\/a>). Each species of eukaryotes has a characteristic number of chromosomes in the nuclei of its cells. Human body (somatic) cells have 46 chromosomes, while human gametes (sperm or eggs) have 23 chromosomes each. A typical body cell contains two matched or homologous sets of chromosomes (one set from each biological parent)\u2014a configuration known as diploid. (Note: The letter <em>n<\/em> is used to represent a single set of chromosomes; therefore, a diploid organism is designated 2<em>n<\/em>.) Human cells that contain one set of chromosomes are called gametes, or sex cells; these are eggs and sperm, and are designated <em>1n<\/em>, or haploid.<\/p>\n<p>Upon fertilization, each gamete contributes one set of chromosomes, creating a diploid cell containing matched pairs of chromosomes called homologous (\u201csame knowledge\u201d) chromosomes. Homologous chromosomes are the same length and have specific nucleotide segments called genes in exactly the same location, or locus. Genes, the functional units of chromosomes, determine specific characteristics by coding for specific proteins. Traits are the variations of those characteristics. For example, hair color is a characteristic with traits that are blonde, brown, or black, and many colors in between.<\/p>\n<div id=\"fig-ch10_01_02\" class=\"wp-caption aligncenter\">\n<div style=\"width: 330px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/s3-us-west-2.amazonaws.com\/courses-images\/wp-content\/uploads\/sites\/3206\/2018\/05\/03181214\/Figure_10_01_02.jpg\" alt=\"The 23 chromosomes from a human female are each dyed a different color so they can be distinguished. During most of the cell cycle, each chromosome is elongated into a thin strand that folds over on itself, like a piece of spaghetti. The chromosomes fill the entire spherical nucleus, but each one is contained in a different part, resulting in a multi-colored sphere. During mitosis, the chromosomes condense into thick, compact bars, each a different color. These bars can be arranged in numerical order to form a karyotype. There are two copies of each chromosome in the karyotype..\" width=\"320\" height=\"430\" \/><\/p>\n<p class=\"wp-caption-text\"><strong>Figure 2. <\/strong>There are 23 pairs of homologous chromosomes in a female human somatic cell. The condensed chromosomes are viewed within the nucleus (top), removed from a cell during mitosis (also called karyokinesis or nuclear division) and spread out on a slide (right), and artificially arranged according to length (left); an arrangement like this is called a karyotype. In this image, the chromosomes were exposed to fluorescent stains for differentiation of the different chromosomes. A method of staining called \u201cchromosome painting\u201d employs fluorescent dyes that highlight chromosomes in different colors. (credit: National Human Genome Project\/NIH)<\/p>\n<\/div>\n<\/div>\n<p id=\"fs-id2048187\">Each copy of a homologous pair of chromosomes originates from a different parent; therefore, the different genes (alleles) themselves are not identical, although they code for the same traits such as \u201chair color.\u201d The variation of individuals within a species is due to the specific combination of the genes inherited from both parents. Even a slightly altered sequence of nucleotides within a gene can result in an alternative trait. For example, there are three possible gene sequences on the human chromosome that code for blood type: sequence A, sequence B, and sequence O. Because all diploid human cells have two copies of the chromosome that determines blood type, the blood type (the trait) is determined by the two alleles of the marker gene that are inherited. It is possible to have two copies of the same gene sequence on both homologous chromosomes, with one on each (for example, AA, BB, or OO), or two different sequences, such as AB, AO, or BO.<\/p>\n<p>Apparently minor variations of traits, such as blood type, eye color, and handedness, contribute to the natural variation found within a species, but even though they seem minor, these traits may be connected with the expression of other traits as of yet unknown. However, if the entire DNA sequence from any pair of human homologous chromosomes is compared, the difference is much less than one percent. The sex chromosomes, X and Y, are the single exception to the rule of homologous chromosome uniformity: Other than a small amount of homology that is necessary to accurately produce gametes, the genes found on the X and Y chromosomes are different.<\/p>\n<\/div>\n<div class=\"bc-section section\">\n<h3>Eukaryotic Chromosomal Structure and Compaction<\/h3>\n<p id=\"fs-id2385346\">If the DNA from all 46 chromosomes in a human cell nucleus were laid out end-to-end, it would measure approximately two meters; however, its diameter would be only 2 nm! Considering that the size of a typical human cell is about 10 \u00b5m (100,000 cells lined up to equal one meter), DNA must be <em>tightly packaged<\/em> to fit in the cell\u2019s nucleus. At the same time, it must also be readily accessible for the genes to be expressed. For this reason, the long strands of DNA are condensed into compact chromosomes during certain stages of the cell cycle. There are a number of ways that chromosomes are compacted.<\/p>\n<p id=\"fs-id1393700\">In the first level of compaction, short stretches of the DNA double helix wrap around a core of eight histone proteins at regular intervals along the entire length of the chromosome (<a class=\"autogenerated-content\" href=\"#fig-ch10_01_03\">(Figure)<\/a>). The DNA-histone complex is called chromatin. The beadlike, histone DNA complex is called a nucleosome, and DNA connecting the nucleosomes is called linker DNA. A DNA molecule in this form is about seven times shorter than the double helix without the histones, and the beads are about 10 nm in diameter, in contrast with the 2-nm diameter of a DNA double helix.<\/p>\n<p id=\"fs-id1373700\">The second level of compaction occurs as the nucleosomes and the linker DNA between them coil into a 30-nm chromatin fiber. This coiling further <em>condenses<\/em> the chromosome so that it is now about 50 times shorter than the extended form.<\/p>\n<p id=\"fs-id1373701\">In the third level of compaction, a variety of <em>fibrous proteins<\/em> is used to \u201cpack the chromatin.\u201d These fibrous proteins also ensure that each chromosome in a non-dividing cell occupies a particular area of the nucleus that does not overlap with that of any other chromosome (see the top image in <a class=\"autogenerated-content\" href=\"#fig-ch10_01_02\">(Figure)<\/a>).<\/p>\n<div id=\"fig-ch10_01_03\" class=\"wp-caption aligncenter\">\n<div style=\"width: 330px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/s3-us-west-2.amazonaws.com\/courses-images\/wp-content\/uploads\/sites\/3206\/2018\/05\/03181217\/Figure_10_01_03.jpg\" alt=\"There are five levels of chromosome organization. From top to bottom: The top panel shows a DNA double helix. The second panel shows the double helix wrapped around proteins called histones. The middle panel shows the entire DNA molecule wrapping around many histones, creating the appearance of beads on a string. The fourth panel shows that the chromatin fiber further condenses into the chromosome shown in the bottom panel.\" width=\"320\" height=\"939\" \/><\/p>\n<p class=\"wp-caption-text\"><strong>Figure 3. <\/strong>Double-stranded DNA wraps around histone proteins to form nucleosomes that create the appearance of \u201cbeads on a string.\u201d The nucleosomes are coiled into a 30-nm chromatin fiber. When a cell undergoes mitosis, the chromosomes condense even further.<\/p>\n<\/div>\n<\/div>\n<p id=\"fs-id1466552\"><em>DNA replicates in the S phase of interphase, which technically is not a part of mitosis, but must always precede it<\/em>. After replication, the chromosomes are composed of two linked sister chromatids. When fully compact, the pairs of identically packed chromosomes are bound to each other by cohesin proteins. The connection between the sister chromatids is closest in a region called the centromere. The conjoined sister chromatids, with a diameter of about 1 \u00b5m, are visible under a light microscope. The centromeric region is highly condensed and thus will appear as a constricted area.<\/p>\n<div id=\"fs-id2919212\" class=\"interactive textbox tryit\">\n<h3>Link to Learning<\/h3>\n<p id=\"fs-id2338602\">This <a href=\"https:\/\/youtu.be\/gbSIBhFwQ4s\">animation<\/a> illustrates the different levels of chromosome packing.<\/p>\n<\/div>\n<\/div>\n<div class=\"summary textbox key-takeaways\">\n<h3>Section Summary<\/h3>\n<p>Prokaryotes have a single circular chromosome composed of double-stranded DNA, whereas eukaryotes have multiple, linear chromosomes composed of chromatin wrapped around histones, all of which are surrounded by a nuclear membrane. The 46 chromosomes of human somatic cells are composed of 22 pairs of autosomes (matched pairs) and a pair of sex chromosomes, which may or may not be matched. This is the 2<em>n<\/em> or diploid state. Human gametes have 23 chromosomes, or one complete set of chromosomes; a set of chromosomes is complete with either one of the sex chromosomes, X or Y. This is the <em>n<\/em> or haploid state. Genes are segments of DNA that code for a specific functional molecule (a protein or RNA). An organism\u2019s traits are determined by the genes inherited from each parent. Duplicated chromosomes are composed of two sister chromatids. Chromosomes are compacted using a variety of mechanisms during certain stages of the cell cycle. Several classes of protein are involved in the organization and packing of the chromosomal DNA into a highly condensed structure. The condensing complex compacts chromosomes, and the resulting condensed structure is necessary for chromosomal segregation during mitosis.<\/p>\n<\/div>\n<div id=\"fs-id1569634\" class=\"multiple-choice textbox exercises\">\n<h3>Review Questions<\/h3>\n<div>\n<div id=\"fs-id1419233\">\n<p id=\"fs-id2641842\">A diploid cell has_______ the number of chromosomes as a haploid cell.<\/p>\n<ol id=\"fs-id2491390\" type=\"a\">\n<li>one-fourth<\/li>\n<li>half<\/li>\n<li>twice<\/li>\n<li>four times<\/li>\n<\/ol>\n<\/div>\n<div class=\"qa-wrapper\" style=\"display: block\"><span class=\"show-answer collapsed\" style=\"cursor: pointer\" data-target=\"qfs-id1982850\">Show Solution<\/span><\/p>\n<div id=\"qfs-id1982850\" class=\"hidden-answer\" style=\"display: none\">\n<div id=\"fs-id1982850\">\n<p id=\"fs-id2909422\">C<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div>\n<div id=\"fs-id3241208\">\n<p id=\"fs-id2335848\">An organism\u2019s traits are determined by the specific combination of inherited _____.<\/p>\n<ol id=\"fs-id2077209\" type=\"a\">\n<li>cells.<\/li>\n<li>genes.<\/li>\n<li>proteins.<\/li>\n<li>chromatids.<\/li>\n<\/ol>\n<\/div>\n<div class=\"qa-wrapper\" style=\"display: block\"><span class=\"show-answer collapsed\" style=\"cursor: pointer\" data-target=\"qfs-id1293208\">Show Solution<\/span><\/p>\n<div id=\"qfs-id1293208\" class=\"hidden-answer\" style=\"display: none\">\n<div id=\"fs-id1293208\">\n<p id=\"fs-id2642965\">B<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div>\n<div id=\"fs-id1447690\">\n<p id=\"fs-id2060998\">The first level of DNA organization in a eukaryotic cell is maintained by which molecule?<\/p>\n<ol id=\"fs-id2165232\" type=\"a\">\n<li>cohesin<\/li>\n<li>condensin<\/li>\n<li>chromatin<\/li>\n<li>histone<\/li>\n<\/ol>\n<\/div>\n<div class=\"qa-wrapper\" style=\"display: block\"><span class=\"show-answer collapsed\" style=\"cursor: pointer\" data-target=\"qfs-id2186440\">Show Solution<\/span><\/p>\n<div id=\"qfs-id2186440\" class=\"hidden-answer\" style=\"display: none\">\n<div id=\"fs-id2186440\">\n<p id=\"fs-id1975120\">D<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"fs-id2315550\">\n<div id=\"fs-id1853884\">\n<p>Identical copies of chromatin held together by cohesin at the centromere are called _____.<\/p>\n<ol type=\"a\">\n<li>histones.<\/li>\n<li>nucleosomes.<\/li>\n<li>chromatin.<\/li>\n<li>sister chromatids.<\/li>\n<\/ol>\n<\/div>\n<div class=\"qa-wrapper\" style=\"display: block\"><span class=\"show-answer collapsed\" style=\"cursor: pointer\" data-target=\"qfs-id1711955\">Show Solution<\/span><\/p>\n<div id=\"qfs-id1711955\" class=\"hidden-answer\" style=\"display: none\">\n<div id=\"fs-id1711955\">\n<p id=\"fs-id1651050\">D<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"free-response textbox exercises\">\n<h3>Free Response<\/h3>\n<div>\n<div>\n<p>Compare and contrast a human somatic cell to a human gamete.<\/p>\n<\/div>\n<div class=\"qa-wrapper\" style=\"display: block\"><span class=\"show-answer collapsed\" style=\"cursor: pointer\" data-target=\"qfs-id3014809\">Show Solution<\/span><\/p>\n<div id=\"qfs-id3014809\" class=\"hidden-answer\" style=\"display: none\">\n<div id=\"fs-id3014809\">\n<p id=\"fs-id2187324\">Human somatic cells have 46 chromosomes: 22 pairs and 2 sex chromosomes that may or may not form a pair. This is the 2<em>n<\/em> or diploid condition. Human gametes have 23 chromosomes, one each of 23 unique chromosomes, one of which is a sex chromosome. This is the <em>n<\/em> or haploid condition.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div>\n<div id=\"fs-id1794020\">\n<p>What is the relationship between a genome, chromosomes, and genes?<\/p>\n<\/div>\n<div class=\"qa-wrapper\" style=\"display: block\"><span class=\"show-answer collapsed\" style=\"cursor: pointer\" data-target=\"qfs-id2739844\">Show Solution<\/span><\/p>\n<div id=\"qfs-id2739844\" class=\"hidden-answer\" style=\"display: none\">\n<div id=\"fs-id2739844\">\n<p>The genome consists of the sum total of an organism\u2019s chromosomes. Each chromosome contains hundreds and sometimes thousands of genes, segments of DNA that code for a polypeptide or RNA, and a large amount of DNA with no known function.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"fs-id1444370\">\n<div id=\"fs-id1595608\">\n<p id=\"fs-id1468864\">Eukaryotic chromosomes are thousands of times longer than a typical cell. Explain how chromosomes can fit inside a eukaryotic nucleus.<\/p>\n<\/div>\n<div class=\"qa-wrapper\" style=\"display: block\"><span class=\"show-answer collapsed\" style=\"cursor: pointer\" data-target=\"qfs-id2643307\">Show Solution<\/span><\/p>\n<div id=\"qfs-id2643307\" class=\"hidden-answer\" style=\"display: none\">\n<div id=\"fs-id2643307\">\n<p>The DNA double helix is wrapped around histone proteins to form structures called nucleosomes. Nucleosomes and the linker DNA in between them are coiled into a 30-nm fiber. During cell division, chromatin is further condensed by packing proteins.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"textbox shaded\">\n<h3>Glossary<\/h3>\n<dl id=\"fs-id2216573\">\n<dt>cell cycle<\/dt>\n<dd id=\"fs-id1293714\">ordered sequence of events through which a cell passes between one cell division and the next<\/dd>\n<\/dl>\n<dl>\n<dt>centromere<\/dt>\n<dd id=\"fs-id1626303\">region at which sister chromatids are bound together; a constricted area in condensed chromosomes<\/dd>\n<\/dl>\n<dl id=\"fs-id2200988\">\n<dt>chromatid<\/dt>\n<dd>single DNA molecule of two strands of duplicated DNA and associated proteins held together at the centromere<\/dd>\n<\/dl>\n<dl>\n<dt>diploid<\/dt>\n<dd>cell, nucleus, or organism containing two sets of chromosomes (2<em>n<\/em>)<\/dd>\n<\/dl>\n<dl id=\"fs-id1693570\">\n<dt>gamete<\/dt>\n<dd id=\"fs-id1976449\">haploid reproductive cell or sex cell (sperm, pollen grain, or egg)<\/dd>\n<\/dl>\n<dl id=\"fs-id2121017\">\n<dt>gene<\/dt>\n<dd id=\"fs-id2011748\">physical and functional unit of heredity, a sequence of DNA that codes for a protein.<\/dd>\n<\/dl>\n<dl id=\"fs-id2150211\">\n<dt>genome<\/dt>\n<dd id=\"fs-id1453713\">total genetic information of a cell or organism<\/dd>\n<\/dl>\n<dl id=\"fs-id1730718\">\n<dt>haploid<\/dt>\n<dd id=\"fs-id1569099\">cell, nucleus, or organism containing one set of chromosomes (<em>n<\/em>)<\/dd>\n<\/dl>\n<dl id=\"fs-id2654173\">\n<dt>histone<\/dt>\n<dd id=\"fs-id2569081\">one of several similar, highly conserved, low molecular weight, basic proteins found in the chromatin of all eukaryotic cells; associates with DNA to form nucleosomes<\/dd>\n<\/dl>\n<dl>\n<dt>homologous chromosomes<\/dt>\n<dd id=\"fs-id1791301\">chromosomes of the same morphology with genes in the same location; diploid organisms have pairs of homologous chromosomes (homologs), with each homolog derived from a different parent<\/dd>\n<\/dl>\n<dl>\n<dt>locus<\/dt>\n<dd id=\"fs-id2626368\">position of a gene on a chromosome<\/dd>\n<\/dl>\n<dl>\n<dt>nucleosome<\/dt>\n<dd id=\"fs-id3032152\">subunit of chromatin composed of a short length of DNA wrapped around a core of histone proteins<\/dd>\n<\/dl>\n<\/div>\n\n\t\t\t <section class=\"citations-section\" role=\"contentinfo\">\n\t\t\t <h3>Candela Citations<\/h3>\n\t\t\t\t\t <div>\n\t\t\t\t\t\t <div id=\"citation-list-761\">\n\t\t\t\t\t\t\t <div class=\"licensing\"><div class=\"license-attribution-dropdown-subheading\">CC licensed content, Shared previously<\/div><ul class=\"citation-list\"><li>Biology 2e. <strong>Provided by<\/strong>: OpenStax. <strong>Located at<\/strong>: <a target=\"_blank\" href=\"https:\/\/openstax.org\/details\/books\/biology-2e\">https:\/\/openstax.org\/details\/books\/biology-2e<\/a>. <strong>License<\/strong>: <em><a target=\"_blank\" rel=\"license\" href=\"https:\/\/creativecommons.org\/licenses\/by\/4.0\/\">CC BY: Attribution<\/a><\/em>. <strong>License Terms<\/strong>: Download for free at http:\/\/cnx.org\/contents\/8d50a0af-948b-4204-a71d-4826cba765b8@8.19<\/li><\/ul><\/div>\n\t\t\t\t\t\t <\/div>\n\t\t\t\t\t <\/div>\n\t\t\t <\/section>","protected":false},"author":311,"menu_order":2,"template":"","meta":{"_candela_citation":"[{\"type\":\"cc\",\"description\":\"Biology 2e\",\"author\":\"\",\"organization\":\"OpenStax\",\"url\":\"https:\/\/openstax.org\/details\/books\/biology-2e\",\"project\":\"\",\"license\":\"cc-by\",\"license_terms\":\"Download for free at http:\/\/cnx.org\/contents\/8d50a0af-948b-4204-a71d-4826cba765b8@8.19\"}]","CANDELA_OUTCOMES_GUID":"","pb_show_title":"on","pb_short_title":"","pb_subtitle":"","pb_authors":[],"pb_section_license":""},"chapter-type":[],"contributor":[],"license":[],"class_list":["post-761","chapter","type-chapter","status-publish","hentry"],"part":718,"_links":{"self":[{"href":"https:\/\/courses.lumenlearning.com\/suny-osbiology2e\/wp-json\/pressbooks\/v2\/chapters\/761","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/courses.lumenlearning.com\/suny-osbiology2e\/wp-json\/pressbooks\/v2\/chapters"}],"about":[{"href":"https:\/\/courses.lumenlearning.com\/suny-osbiology2e\/wp-json\/wp\/v2\/types\/chapter"}],"author":[{"embeddable":true,"href":"https:\/\/courses.lumenlearning.com\/suny-osbiology2e\/wp-json\/wp\/v2\/users\/311"}],"version-history":[{"count":3,"href":"https:\/\/courses.lumenlearning.com\/suny-osbiology2e\/wp-json\/pressbooks\/v2\/chapters\/761\/revisions"}],"predecessor-version":[{"id":2488,"href":"https:\/\/courses.lumenlearning.com\/suny-osbiology2e\/wp-json\/pressbooks\/v2\/chapters\/761\/revisions\/2488"}],"part":[{"href":"https:\/\/courses.lumenlearning.com\/suny-osbiology2e\/wp-json\/pressbooks\/v2\/parts\/718"}],"metadata":[{"href":"https:\/\/courses.lumenlearning.com\/suny-osbiology2e\/wp-json\/pressbooks\/v2\/chapters\/761\/metadata\/"}],"wp:attachment":[{"href":"https:\/\/courses.lumenlearning.com\/suny-osbiology2e\/wp-json\/wp\/v2\/media?parent=761"}],"wp:term":[{"taxonomy":"chapter-type","embeddable":true,"href":"https:\/\/courses.lumenlearning.com\/suny-osbiology2e\/wp-json\/pressbooks\/v2\/chapter-type?post=761"},{"taxonomy":"contributor","embeddable":true,"href":"https:\/\/courses.lumenlearning.com\/suny-osbiology2e\/wp-json\/wp\/v2\/contributor?post=761"},{"taxonomy":"license","embeddable":true,"href":"https:\/\/courses.lumenlearning.com\/suny-osbiology2e\/wp-json\/wp\/v2\/license?post=761"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}