DNA Structure and Function (Instructor Materials Preparation)

Lab Materials

This is the prep for one section of 24 students.

Part 4: Wheat Germ Extraction

Students will do this part in pairs.

Materials Quantity Notes
Beaker (250 mL) 1 per table
Graduated cylinder (10 mL) 1 per table
Graduated cylinder (100 mL) 1 per table
Serological pipettes (5 mL) 1 per table 2 additional for sodium bicarbonate solution
Pipettors (green) 1 per table 2 additional for sodium bicarbonate solution
Thermometers 1 per table
Dawn dish detergent 1 per table Small personal container
Adolf’s Meat Tenderizer (original unseasoned) 2 containers On side counter
Scoops for meat tenderizer 2 On side counter
Raw untoasted wheat germ 2 containers On side counter
Digital balance 2 On side counter
Weigh papers On side counter
1 M sodium bicarbonate ~2 bottles Each pair will need 5 mL
Waste buckets for wheat germ 2
Signs across sinks: “No used wheat germ disposal or you will be destroyed!”
Glass DNA hooks 6 At instructor’s station
Ice bath On side counter; large enough for 10 beakers
Hot bath On side counter with thermometer; 55º C
Alcohol (95% in freezer) 20 mL per table On side counter in ice bath
Glass stirring rods 1 per table
Wax pencils 1 per table