Lab Materials
This is the prep for one section of 24 students.
Part 1: Starch Synthesis and Storage
Students will do this part in pairs.
| Materials | Quantity | Notes |
|---|---|---|
| Microscope slides | 1 per student | |
| Coverslips | 1 per student | |
| Iondine (I2KI) | 1 per table | Dropper bottles |
| Potato slices | 1 small piece is fine | On side counter with cutting board and knife/razor blade |
Part 2: Starch Digestion
Students will do this part in table teams.
| Materials | Quantity | Notes |
|---|---|---|
| Ehrlenmeyer flask (250 mL) | 2 per table | |
| Glass stirring rod | 2 per table | |
| 10 mL graduated cylinder | 1 per table | |
| Hot plate | 1 per table | |
| Hot pads | 2 per table | |
| Corn starch | 20g per table | On side counter |
| Weigh paper and balance | 4 | On side counter |
| Glucose test strips | 2 per table | On side counter |
| Wax pencil | 1 per table | Also used in Part 3 |
| Amylase | 2 mL per table | On side or front counter |
| 250 mL beaker | (So students can measure the water) |
Part 3: Aerobic Respiration
Students will do this part in table teams.
| Materials | Quantity | Notes |
|---|---|---|
| Respiration apparatus (funnel attached to stopper with glass tubing) |
1 per table | (Plus one for front teacher station) |
| Ehrlenmeyer flasks (to fit respiration apparatus)—500 mL |
3 per table | (Plus one for front teacher station) |
| Rubber stoppers (No. 7) | 2 per table | (Plus one for front teacher station) |
| Large test tubes | 6 per table | Also used in Part 4 (Plus one for front teacher station) |
| Test tube racks | 1 per table | Also used in Part 4 (Plus one for front teacher station) |
| Phenol red dropper bottles (2%) | 1 per table | |
| Cooked germinated pinto beans | 1 large culture dish | To prep beans: soak for 1–2 nights, covered in wet paper towels to allow germination. On day of lab, boil half the beans for 3–4 minutes then cool down to room temp. |
| Uncooked germinated pinto beans | 1 large culture dish | |
| Handful of dry pinto beans | For front table | |
| Waste bucket for beans | 2 | On side counter |
| Slotted spoons | 2 | One in each dish of beans |
Part 4: Anaerobic Respiration
Students will do this part in table teams.
| Materials | Quantity | Notes |
|---|---|---|
| Microspatulas for stirring in tubes | 1 per table | |
| Water bath (37˚ C) with thermometer | 1 | Side counter |
| Water bath (55˚ C) with thermometer | 1 | Side counter |
| Ice bath | 1 | Side counter |
| Yeast (in fridge) | 2 | Side counter |
| Sugar: Lactose (with labeled scoop) | 2 culture dishes | Side counter |
| Sugar: Glucose (with labeled scoop) | 2 culture dishes | Side counter |
| Sugar: Sucrose (with labeled scoop) | 2 culture dishes | Side counter |
| Sugar: Dextrin (with labeled scoop) | 2 culture dishes | Side counter |
| Tubs for used test tubes | 1 tub | |
| Balloons (9 inch) | 5 per table | |
| Cloth measuring tapes | 1 per table | |
| Rubber bands for test tubes (to keep balloons on) | 5 per table |