Table of Contents

• • Preface

• Instructor Resources

• An Invisible World

  • Introduction to An Invisible World
  • What Our Ancestors Knew
  • A Systematic Approach
  • Types of Microorganisms

• How We See the Invisible World

  • Introduction to How We See the Invisible World
  • The Properties of Light
  • Peering Into the Invisible World
  • Instruments of Microscopy
  • Staining Microscopic Specimens

• The Cell

  • Introduction to the Cell
  • Spontaneous Generation
  • Foundations of Modern Cell Theory
  • Unique Characteristics of Prokaryotic Cells
  • Unique Characteristics of Eukaryotic Cells

• Prokaryotic Diversity

  • Introduction to Prokaryotic Diversity
  • Prokaryote Habitats, Relationships, and Microbiomes
  • Proteobacteria
  • Nonproteobacteria Gram-Negative Bacteria and Phototrophic Bacteria
  • Gram-Positive Bacteria
  • Deeply Branching Bacteria
  • Archaea

• The Eukaryotes of Microbiology

  • Introduction to the Eukaryotes of Microbiology
  • Unicellular Eukaryotic Parasites
  • Parasitic Helminths
  • Fungi
  • Algae
  • Lichens

• Acellular Pathogens

  • Introduction to Acellular Pathogens
  • Viruses
  • The Viral Life Cycle
  • Isolation, Culture, and Identification of Viruses
  • Viroids, Virusoids, and Prions

• Microbial Biochemistry

  • Introduction to Microbial Biochemistry
  • Organic Molecules
  • Carbohydrates
  • Lipids
  • Proteins
  • Using Biochemistry to Identify Microorganisms

• Microbial Metabolism

  • Introduction to Microbial Metabolism
  • Energy, Matter, and Enzymes
  • Catabolism of Carbohydrates
  • Cellular Respiration
  • Fermentation
  • Catabolism of Lipids and Proteins
  • Photosynthesis
  • Biogeochemical Cycles

• Microbial Growth

  • Introduction to Microbial Growth
  • How Microbes Grow
  • Oxygen Requirements for Microbial Growth
  • The Effects of pH on Microbial Growth
  • Temperature and Microbial Growth
  • Other Environmental Conditions that Affect Growth
  • Media Used for Bacterial Growth

• Biochemistry of the Genome

  • Introduction to Biochemistry of the Genome
  • Using Microbiology to Discover the Secrets of Life
  • Structure and Function of DNA
  • Structure and Function of RNA
  • Structure and Function of Cellular Genomes

• Mechanisms of Microbial Genetics

  • Introduction to Mechanisms of Microbial Genetics
  • The Functions of Genetic Material
  • DNA Replication
  • RNA Transcription
  • Protein Synthesis (Translation)
  • Mutations
  • How Asexual Prokaryotes Achieve Genetic Diversity
  • Gene Regulation: Operon Theory

• Modern Applications of Microbial Genetics

  • Introduction to Modern Applications of Microbial Genetics
  • Microbes and the Tools of Genetic Engineering
  • Visualizing and Characterizing DNA, RNA, and Protein
  • Whole Genome Methods and Pharmaceutical Applications of Genetic Engineering
  • Gene Therapy

• Control of Microbial Growth

  • Introduction to Control of Microbial Growth
  • Controlling Microbial Growth
  • Using Physical Methods to Control Microorganisms
  • Using Chemicals to Control Microorganisms
  • Testing the Effectiveness of Antiseptics and Disinfectants

• Antimicrobial Drugs

  • Introduction to Antimicrobial Drugs
  • History of Chemotherapy and Antimicrobial Discovery
  • Fundamentals of Antimicrobial Chemotherapy
  • Mechanisms of Antibacterial Drugs
  • Mechanisms of Other Antimicrobial Drugs
  • Drug Resistance
  • Testing the Effectiveness of Antimicrobials
  • Current Strategies for Antimicrobial Discovery

• Microbial Mechanisms of Pathogenicity

  • Introduction to Microbial Mechanisms of Pathogenicity
  • Characteristics of Infectious Disease
  • How Pathogens Cause Disease
  • Virulence Factors of Bacterial and Viral Pathogens
  • Virulence Factors of Eukaryotic Pathogens

• Disease and Epidemiology

  • Introduction to Disease and Epidemiology
  • The Language of Epidemiologists
  • Tracking Infectious Diseases
  • Modes of Disease Transmission
  • Global Public Health

• Innate Nonspecific Host Defenses

  • Introduction to Innate Nonspecific Host Defenses
  • Physical Defenses
  • Chemical Defenses
  • Cellular Defenses
  • Pathogen Recognition and Phagocytosis
  • Inflammation and Fever

• Adaptive Specific Host Defenses

  • Introduction to Adaptive Specific Host Defenses
  • Overview of Specific Adaptive Immunity
  • Major Histocompatibility Complexes and Antigen-Presenting Cells
  • T Lymphocytes and Cellular Immunity
  • B Lymphocytes and Humoral Immunity
  • Vaccines

• Diseases of the Immune System

  • Introduction
  • Hypersensitivities
  • Autoimmune Disorders
  • Organ Transplantation and Rejection
  • Immunodeficiency
  • Cancer Immunobiology and Immunotherapy

• Laboratory Analysis of the Immune Response

  • Introduction to Laboratory Analysis of the Immune Response
  • Polyclonal and Monoclonal Antibody Production
  • Detecting Antigen-Antibody Complexes
  • Agglutination Assays
  • EIAs and ELISAs
  • Fluorescent Antibody Techniques

• Skin and Eye Infections

  • Introduction
  • Anatomy and Normal Microbiota of the Skin and Eyes
  • Bacterial Infections of the Skin and Eyes
  • Viral Infections of the Skin and Eyes
  • Mycoses of the Skin
  • Protozoan and Helminthic Infections of the Skin and Eyes

• Respiratory System Infections

  • Introduction to Respiratory System Infections
  • Anatomy and Normal Microbiota of the Respiratory Tract
  • Bacterial Infections of the Respiratory Tract
  • Viral Infections of the Respiratory Tract
  • Respiratory Mycoses

• Urogenital System Infections

  • Introduction to Urogenital System Infections
  • Anatomy and Normal Microbiota of the Urogenital Tract
  • Bacterial Infections of the Urinary System
  • Bacterial Infections of the Reproductive System
  • Viral Infections of the Reproductive System
  • Fungal Infections of the Reproductive System
  • Protozoan Infections of the Urogenital System

• Digestive System Infections

  • Introduction
  • Anatomy and Normal Microbiota of the Digestive System
  • Microbial Diseases of the Mouth and Oral Cavity
  • Bacterial Infections of the Gastrointestinal Tract
  • Viral Infections of the Gastrointestinal Tract
  • Protozoan Infections of the Gastrointestinal Tract
  • Helminthic Infections of the Gastrointestinal Tract

• Circulatory and Lymphatic System Infections

  • Introduction to Circulatory and Lymphatic System Infections
  • Anatomy of the Circulatory and Lymphatic Systems
  • Bacterial Infections of the Circulatory and Lymphatic Systems
  • Viral Infections of the Circulatory and Lymphatic Systems
  • Parasitic Infections of the Circulatory and Lymphatic Systems

• Nervous System Infections

  • Introduction
  • Anatomy of the Nervous System
  • Bacterial Diseases of the Nervous System
  • Acellular Diseases of the Nervous System
  • Fungal and Parasitic Diseases of the Nervous System

• Appendices

  • Fundamentals of Physics and Chemistry Important to Microbiology
  • Mathematical Basics
  • Metabolic Pathways
  • Taxonomy of Clinically Relevant Microorganisms
  • Glossary